Molecular Marker Aided Selection of Restorer of Fertility (Rf/rf) Alleles to Develop Maintainer Lines in Sunflower

Basit Öğe Kaydı
dc.authoridMUTLU, Nedim/0000-0001-7252-5883
dc.authoridPakashtica, Vese/0000-0001-5943-5269
dc.authoridsever mutlu, songul/0000-0002-7886-1594;
dc.contributor.authorZailasheva, Array
dc.contributor.authorSancar, Bahar
dc.contributor.authorZonon, Mamoudou
dc.contributor.authorKarabeniz, Alparslan
dc.contributor.authorPakashtica, Vese
dc.contributor.authorSever Mutlu, Songuel
dc.contributor.authorMutlu, Nedim
dc.date.accessioned2025-10-11T20:47:58Z
dc.date.available2025-10-11T20:47:58Z
dc.date.issued2025
dc.departmentDüzce Üniversitesien_US
dc.description.abstractSunflower (Helianthus annuus L.), one of the main oil crops in the world, is also increasingly used for its ornamental value. Marker-assisted selection (MAS) helps identify desired traits through molecular markers linked to specific genes, speeding up the breeding process. In sunflower breeding, dominant and codominant markers are used to detect Rf1/rf1 alleles for the selection of plants carrying maintainer and/or restorer (Rf) nuclei. In a breeding program, the status of Rf/rf alleles in the nucleus of the maintainer (B) lines cannot be ascertained without a test-cross due to the presence of fertile cytoplasm. The aim of this study was to test the utilization of the SCAR markers linked to Rf/rf alleles in sunflower to test if the markers can be integrated into a breeding program to develop maintainer lines. The plant materials included restorer and maintainer lines, as well as backcross and test-cross populations segregating for Rf/rf alleles. The markers (PPR621.5) have proven to be valuable for MAS in sunflower breeding. The Rf/rf specific dominant markers were used with the atp9 internal control marker to differentiate PCR failure from the absence of an allele. The markers were also phenotypically confirmed using advanced lines, BC1F1, BC2F1, and BC1F2, as well as test-cross populations. The application of SCAR markers in developing new restorer and maintainer lines can enable efficient determination of Rf/rf allele status, thereby eliminating the need for test crosses in maintainer nuclei and significantly enhancing breeding efficiency. Detection of Rf/rf alleles by molecular markers in maintainer populations with fertile cytoplasm may help reduce cost and breeding time to develop maintainer lines.en_US
dc.identifier.doi10.17557/tjfc.1595579
dc.identifier.endpage97en_US
dc.identifier.issn1301-1111
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-105009436248en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage88en_US
dc.identifier.urihttps://doi.org/10.17557/tjfc.1595579
dc.identifier.urihttps://hdl.handle.net/20.500.12684/21675
dc.identifier.volume30en_US
dc.identifier.wosWOS:001494567400002en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSoc Field Crop Scien_US
dc.relation.ispartofTurkish Journal of Field Cropsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmzKA_WOS_20250911
dc.subjectCMSen_US
dc.subjectMaintaineren_US
dc.subjectMASen_US
dc.subjectRestoreren_US
dc.subjectSCARen_US
dc.subjectSunfloweren_US
dc.titleMolecular Marker Aided Selection of Restorer of Fertility (Rf/rf) Alleles to Develop Maintainer Lines in Sunfloweren_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu