A Comparison of Triple and Double Sperm Washing for Density Gradient Preparation in Intrauterine Insemination Cycles when Overnight Incubation of Specimens Occurred: A Retrospective Cohort

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dc.contributor.authorSoyer-Çalışkan, Canan
dc.contributor.authorHatirnaz, Kaan
dc.contributor.authorÇelik, Samettin
dc.contributor.authorBaşbuğ, Alper
dc.contributor.authorHatirnaz, Ebru S.
dc.contributor.authorHatırnaz, Şafak
dc.contributor.authorDahan, Michael H.
dc.date.accessioned2023-07-26T11:50:36Z
dc.date.available2023-07-26T11:50:36Z
dc.date.issued2022
dc.departmentDÜ, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümü, Kadın Hastalıkları ve Doğum Ana Bilim Dalıen_US
dc.description.abstractBackground: The number of sperm washes to maximize outcomes for intra-uterine insemination has not been well investigated. Therefore, we undertook to compare the pregnancy and live birth rates of triple sperm washing and double sperm washing for density gradient preparation for intrauterine insemination (IUI) cycles. Methods: A retrospective cohort study including 279 couples (136 couples with triple sperm washing and 143 couples with double sperm washing) with a diagnosis of unexplained infertility and mild male subfertility who had IUI cycles between April 2015 and April 2017 were evaluated. After overnight incubation of the sperm, subjects underwent either traditional double sperm washing or Triple sperm washing which consists of use of a third gradient and spinning procedure to the conventional double gradient sperm washing in order to obtain a higher quantity of motile sperm. Results: Total sperm count after triple washing was higher than double sperm washing (98.25 +/- 62.06 vs. 81.08 +/- 31.57; p = 0.003). Positive beta-hCG test and live birth per cycle were higher in triple sperm washing (25.8% vs. 13.3%, p = 0.009; 18.4% vs. 9.8%, p = 0.039; respectively) than in the double sperm washing group. Conclusions: The use of motile sperms obtained from triple sperm washing may increase the rates of pregnancy and live birth in IUI cycles of women with unexplained and mild male factor infertility. A prospective randomized study should be undertaken to confirm the results.en_US
dc.identifier.doi10.31083/j.ceog4911250
dc.identifier.issn0390-6663
dc.identifier.issn2709-0094
dc.identifier.issue11en_US
dc.identifier.scopus2-s2.0-85143752401en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.urihttps://doi.org/10.31083/j.ceog4911250
dc.identifier.urihttps://hdl.handle.net/20.500.12684/12385
dc.identifier.volume49en_US
dc.identifier.wosWOS:000917832300015en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorBaşbuğ, Alper
dc.language.isoenen_US
dc.publisherImr Pressen_US
dc.relation.ispartofClinical and Experimental Obstetrics & Gynecologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmz$2023V1Guncelleme$en_US
dc.subjectIntrauterine Insemination; Live Birth; Pregnancy; Infertilityen_US
dc.subjectPregnancy Rates; Dna-Damage; Infertility; Technology; Couples; Timeen_US
dc.titleA Comparison of Triple and Double Sperm Washing for Density Gradient Preparation in Intrauterine Insemination Cycles when Overnight Incubation of Specimens Occurred: A Retrospective Cohorten_US
dc.typeArticleen_US

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