Comparative analysis of embryo surrounding region (Esr-6) genes in Turkish maize varieties: sequencing and modeling

Basit Öğe Kaydı
dc.contributor.authorTombuloğlu, Hüseyin
dc.contributor.authorAydın, Mehtap
dc.contributor.authorFiliz, Ertuğrul
dc.date.accessioned2020-04-30T22:41:05Z
dc.date.available2020-04-30T22:41:05Z
dc.date.issued2016
dc.departmentDÜ, Çilimli Meslek Yüksekokulu, Bitkisel ve Hayvansal Üretim Bölümüen_US
dc.descriptionTombuloglu, Huseyin/0000-0001-8546-2658; Filiz, Ertugrul/0000-0001-9636-6389en_US
dc.descriptionWOS: 000373897100029en_US
dc.description.abstractDefensins are cysteine-rich small proteins found in plants and animals. Esr-6 (embryo surrounding region-6), which is a kind of plant defensin gene, plays an important role in nutrition, defense, and signaling of embryo. In this study, Esr-6 genes in three Turkish maize varieties (TMVs) such as Safak, Sakarya, and Seker were sequenced and characterized. Open reading frame (ORF) and protein length of Esr-6 was found as 324 bp and 111 amino acids, respectively. All TMVs contained gamma-thionin family (PF00304) domain, and subcellular localizations were predicted as extracellular. Predicted signal peptide, mature protein, and acidic pro-domain were identified in all TMVs. Four most conserved motifs were identified, but only motif 1 was found to be related with gamma-thionin family. Eight conserved cysteine residues, which are the characteristics of defensins, were detected in all TMVs. In phylogenetic analysis, Esr-6 proteins were clearly diverged from other plant defensins with the highest bootstrap value (100 %). Predicted 3D structures of Esr-6 proteins were similar to each other, including one a-helix and two beta-sheets. Conserved cysteine residues, including Cys51, Cys55, Cys66, Cys73, Cys75, and Cys79, were detected in 3D structures of all TMVs. Our study showed that Esr-6 genes and their product are well conserved among TMVs.en_US
dc.identifier.doi10.1007/s40415-015-0206-2en_US
dc.identifier.endpage293en_US
dc.identifier.issn0100-8404
dc.identifier.issn1806-9959
dc.identifier.issue1en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage287en_US
dc.identifier.urihttps://doi.org/10.1007/s40415-015-0206-2
dc.identifier.urihttps://hdl.handle.net/20.500.12684/3119
dc.identifier.volume39en_US
dc.identifier.wosWOS:000373897100029en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSoc Botanica Sao Pauloen_US
dc.relation.ispartofBrazilian Journal Of Botanyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBioinformaticsen_US
dc.subjectDefensinen_US
dc.subjectIn silico analysisen_US
dc.subject3D structureen_US
dc.titleComparative analysis of embryo surrounding region (Esr-6) genes in Turkish maize varieties: sequencing and modelingen_US
dc.typeArticleen_US

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim Yok
İsim:
3119.pdf
Boyut:
1.43 MB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin / Full Text
İndir

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Makale Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu