Sulfite Reductase (SiR) Gene in Rice (Oryza sativa): Bioinformatics and Expression Analyses Under Salt and Drought Stresses

Simple item page
dc.authoridFiliz, Ertugrul/0000-0001-9636-6389
dc.authorwosidFiliz, Ertugrul/I-9440-2016
dc.contributor.authorKurt, Firat
dc.contributor.authorFiliz, Ertugrul
dc.contributor.authorAydin, Adnan
dc.date.accessioned2021-12-01T18:49:34Z
dc.date.available2021-12-01T18:49:34Z
dc.date.issued2021
dc.department[Belirlenecek]en_US
dc.description.abstractRice sulfite reductase (OsSiR) is important protein in reducing sulfite to sulfide. In this paper, it is aimed to shed light on OsSiR's probable structure, function, and expression using in silico methods and test its responses under drought and salt stresses. Moreover, it was also analyzed if OsSiR was structurally different from other SiR proteins. We estimated that OsSiR lacks ribbon-helix-helix DNA-binding motif allowing it to bind to DNA; therefore, it was probably localized in stroma as a non-nucleoid-type protein. Also, we found that OsSiR expression was regulated by JA in roots and by crosstalk of JA and ABA in shoots. RT-qPCR results showed that there was 20% increase in the expression of OsSiR at 3rd h of the salt treatment. However, OsSiR was downregulated when exposed to drought stress and salt stress for longer periods of time, respectively. OsSiR has a high post-translational potential because of its high phosphorylation sites. This may be originating from the most prevalent residue, Gly, facilitating its binding to phosphates in OsSiR. Our docking results showed that ligand binding residues of OsSiR (Arg159, Thr162, Gln167, and Pro501) were also active site residues of OsSiR. Both two domains of OsSiR interacted with sulfite and the number of the residues in 4Fe-4S domain (PF01077) was higher. The findings in this study are important in terms of structural and expressional studies of rice SiR (OsSiR) and can be used for SiR proteins in sorghum (Sorghum bicolor), maize (Zea mays), and foxtail millet (Setaria italica), which are closely related and highly similar to OsSiR in terms of sequence and predicted 3D structure.en_US
dc.identifier.doi10.1007/s00344-021-10438-8
dc.identifier.issn0721-7595
dc.identifier.issn1435-8107
dc.identifier.scopus2-s2.0-85110771478en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1007/s00344-021-10438-8
dc.identifier.urihttps://hdl.handle.net/20.500.12684/10742
dc.identifier.wosWOS:000674224400001en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofJournal Of Plant Growth Regulationen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectSulphuren_US
dc.subjectOryza sativaen_US
dc.subjectSulfite reductaseen_US
dc.subjectBioinformaticsen_US
dc.subjectMolecular dockingen_US
dc.subjectProtein-Phosphorylationen_US
dc.subjectScoring Functionen_US
dc.subjectAssimilationen_US
dc.subjectPredictionen_US
dc.subjectSequenceen_US
dc.subjectDatabaseen_US
dc.subjectGrowthen_US
dc.subjectPcren_US
dc.titleSulfite Reductase (SiR) Gene in Rice (Oryza sativa): Bioinformatics and Expression Analyses Under Salt and Drought Stressesen_US
dc.typeArticleen_US

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
10742.pdf
Size:
4.09 MB
Format:
Adobe Portable Document Format
Description:
Tam Metin / Full Text
Download

Collections

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu