In Vitro Evaluation of the Combination Activity of Carvacrol and Oxacillin against Methicillin-Resistant Staphylococcus aureus Strains
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Tarih
2020
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info:eu-repo/semantics/openAccess
Özet
Aim: Methicillin-resistant Staphylococcus aureus (MRSA) is one of the most resistant bacteria to antibiotics. Manyantibiotics are known to be insufficient to treat infections caused by these bacteria. Plant-derived antibacterials havedrawn more attention as a source of new therapeutics. Carvacrol is a monoterpenic phenol compound found in variousessential oils and shows antimicrobial activity against many pathogens. In this study, the combination activity ofcarvacrol and oxacillin against ten MRSA clinical strains was evaluated.Material and Methods: To determine Minimum Inhibitory Concentrations (MIC) of carvacrol and oxacillin against tenMRSA clinical strains, broth microdilution method was performed. The combination activity of carvacrol and oxacillinwas determined with checkerboard synergy test. Whether both antimicrobials and their combinations caused membranedamage in MRSA-6 strain was detected by measuring the amount of nucleic acid leaking out of the cell acrossmembrane with UV spectrophotometer.Results: Carvacrol showed antibacterial activity against all MRSA strains with MIC values in the range of 64-256µg/ml. The synergistic effect (FICI?0.5) between carvacrol and oxacillin was determined against seven strains.Carvacrol caused a membrane damage on MRSA-6 strain. As a result of combination with oxacillin, the increase inthe membrane damage of MRSA-6 strain was found to be statistically significant (p<0.001).Conclusion: According to the results of this study, carvacrol increased the antibacterial effect of oxacillin againstMRSA strains. Thus, carvacrol can be used in combination with oxacillin against MRSA as a novel antimicrobial agent.However, the results of this study should be supported by further studies.
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Düzce Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi
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Cilt
10
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2
