Investigation of nasal colonization by coagulase-positive staphylococci and methicillin resistance in dogs

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dc.authoridkekec, A.Ilgin/0000-0002-0821-8376
dc.authorwosidkekec, A.Ilgin/AFV-8624-2022
dc.contributor.authorCoşkun, Tuğçe
dc.contributor.authorKekeç, Ayşe İlgin
dc.contributor.authorSipahi, Nisa
dc.contributor.authorİkiz, Serkan
dc.date.accessioned2023-07-26T11:54:24Z
dc.date.available2023-07-26T11:54:24Z
dc.date.issued2022
dc.departmentDÜ, Rektörlük, Geleneksel ve Tamamlayıcı Tıp Uygulama ve Araştırma Merkezien_US
dc.description.abstractThis study aimed to determine the presence, rate, and species distribution of coagulase-positive staphylococci (CPS) in nasal swab samples from dogs and to investigate phenotypic and genotypic methicillin resistance. To this end, 21 (10.5%) CPS, including 7 (33.3%) S. aureus and 14 (66.6%) S. intermedius group (SIG), were isolated from nasal swab samples from 200 dogs. A total of 14 SIG members (100%) were also identified as S. pseudintermedius by multiplex polymerase chain reaction (m-PCR). Phenotypic methicillin resistance was observed in 16 (76.19%) of the 21 CPS isolated from 200 dogs from 8 different clinics and shelters. To detect genotypic methicillin resistance, the presence of the mecA and mecC genes, responsible for methicillin resistance, was detected by the multiplex polymerase chain reaction method. The mecA gene was found in 8 (38.09%) of the 21 isolates. The mecC gene was not detected in any of the samples, including isolates showing phenotypic methicillin resistance. It was found that neither the presence of CPS nor the presence of the mecA gene was statistically correlated with age, sex, or antibiotic use in the previous year (p > 0.05). Furthermore, CPS and mecA-positive isolates were evaluated according to whether they were obtained from shelters or clinics. It was concluded that the source of the isolates was not important for our study (p > 0.05). Our findings suggest that phenotypic resistance detected in epidemiological studies should be confirmed by molecular methods. At the same time, the fact that mecC gene-positive staphylococcal isolates were not detected in our study is promising for Turkey.en_US
dc.description.sponsorshipScientific Research Project Coordination Unit of Istanbul University-Cerrahpasa [27790]en_US
dc.description.sponsorshipThis work was supported by the Scientific Research Project Coordination Unit of Istanbul University-Cerrahpasa. (Project no: 27790).en_US
dc.identifier.doi10.21521/mw.6639
dc.identifier.issn0025-8628
dc.identifier.issue4en_US
dc.identifier.scopus2-s2.0-85126113777en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.urihttps://doi.org/10.21521/mw.6639
dc.identifier.urihttps://hdl.handle.net/20.500.12684/12819
dc.identifier.volume78en_US
dc.identifier.wosWOS:000758251800002en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorSipahi, Nisa
dc.language.isoenen_US
dc.publisherPolish Soc Veterinary Sciences Editorial Officeen_US
dc.relation.ispartofMedycyna Weterynaryjna-Veterinary Medicine-Science and Practiceen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmz$2023V1Guncelleme$en_US
dc.subjectCoagulase-Positive Staphylococcus; Dog; Meca; Meccen_US
dc.subjectAntimicrobial Resistance; Species Distribution; Otitis-Externa; Aureus; Intermedius; Carriage; Gene; Pet; Susceptibility; Identificationen_US
dc.titleInvestigation of nasal colonization by coagulase-positive staphylococci and methicillin resistance in dogsen_US
dc.typeArticleen_US

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