PREPARING AND OPTIMIZING A NEWLY AFFINITY GEL FOR PURIFICATION OF CARBONIC ANHYDRASE ISOENZYMES

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dc.contributor.authorUzunoglu, Serap Beyaztas
dc.contributor.authorYalcin, Yildiz
dc.contributor.authorUzunoglu, Tayfun
dc.contributor.authorKaradeniz, Seref
dc.contributor.authorKurt, Habibe
dc.date.accessioned2021-12-01T18:47:52Z
dc.date.available2021-12-01T18:47:52Z
dc.date.issued2020
dc.department[Belirlenecek]en_US
dc.description.abstractHuman carbonic anhydrase isozymes have been purified from the hemolysate, directly by using the original affinity gel in chemical structure of Sepharose 4B-L-tyrosine- sulfathiazole. Different solution buffers were used for obtaining the purified CA isozymes from the affinity column. Most suitable elution buffers were determined for CA I and CA II isoenzymes pH 6.3, 25 mM Na2HPO4/1.0 M NaCl and pH 5.6, 0.1 M NaCH3COO / 0.5 M NaClO4 respectively. The purification values for CA I and CA II have been obtained as 635.71 and 666.71 folds with 28.41% and 46.19% yield respectively. The ionic strength and optimum pH values of the original affinity gel have been determined for each isozyme of CA. Maximum binding was achieved 0.3 ionic strength and pH 8.7 for both CA I and CA II. The single bands was indicated for each isoenzymes by SDS-polyacrylamide gel electrophoresis.en_US
dc.description.sponsorshipTUBITAK with 2209-A - University Student Research Project Support ProgramTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK)en_US
dc.description.sponsorshipThis work has been supported by TUBITAK with 2209-A - University Student Research Project Support Program.en_US
dc.identifier.endpage3803en_US
dc.identifier.issn1018-4619
dc.identifier.issn1610-2304
dc.identifier.issue5en_US
dc.identifier.scopus2-s2.0-85091914552en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage3798en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12684/10398
dc.identifier.volume29en_US
dc.identifier.wosWOS:000535839400053en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherParlar Scientific Publications (P S P)en_US
dc.relation.ispartofFresenius Environmental Bulletinen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCarbonic anhydraseen_US
dc.subjectisozymesen_US
dc.subjectaffinity gelen_US
dc.subjectaffinity chromatographyen_US
dc.subjectpurificationen_US
dc.subjectIsozyme-Xiien_US
dc.subjectInhibitionen_US
dc.subjectSulfonamidesen_US
dc.subjectIxen_US
dc.titlePREPARING AND OPTIMIZING A NEWLY AFFINITY GEL FOR PURIFICATION OF CARBONIC ANHYDRASE ISOENZYMESen_US
dc.typeArticleen_US

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