Effects of Different Doses of Systemic Isotretinoin on Eyes: A Histopathological and Immunohistochemical Study in Rats

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dc.contributor.authorKaradağ, Remzi
dc.contributor.authorKaradağ, Ayşe Serap
dc.contributor.authorÖzlü, Emin
dc.contributor.authorOğuztüzün, Serpil
dc.contributor.authorŞimşek, Gülçin
dc.contributor.authorEsmer, Oktay
dc.contributor.authorBilgili, Serap Güneş
dc.date.accessioned2020-04-30T13:32:23Z
dc.date.available2020-04-30T13:32:23Z
dc.date.issued2020
dc.departmentDÜ, Tıp Fakültesi, Dahili Tıp Bilimleri Bölümüen_US
dc.descriptionPubMed ID: 31842038en_US
dc.description.abstractPURPOSE: To evaluate ocular side effects associated with systemic isotretinoin histopathologically. METHODS: In this multicenter study, a total of 15 male and 15 female rats were randomly divided into 3 equal groups according to the oral dose of isotretinoin they were administered: 0 mg/kg/d (group A), 7.5 mg/kg/d (group B), and 15 mg/kg/d (group C). Biopsy specimens were taken from the globe conjunctiva, cornea, and eyelid conjunctiva. Expression levels of human beta-defensin-1, human beta-defensin-2, toll-like receptor (TLR)-2, and TLR-4 were evaluated by immunohistochemical methods. RESULTS: The number of goblet cells in eyelid conjunctiva was significantly lower in group B than that in group A and group C (P = 0.002). The sizes of meibomian gland acini were significantly smaller in group B and group C than those in group A (P < 0.001). Fibrosis of eyelid conjunctiva was significantly higher in group C and group B than that in group A (P = 0.002). The levels of staining of TLR-4 in the cornea with group B were significantly lower compared with group C (P = 0.035). CONCLUSIONS: Our study suggests that isotretinoin in the early period affects eyelid conjunctiva and meibomian glands without affecting the globe conjunctiva and cornea. Occurrence of the initial symptoms of isotretinoin on the eyelids, especially on the meibomian glands, suggests that the symptoms of patients occur because of evaporative dry eye.en_US
dc.identifier.doi10.1097/ICO.0000000000002229en_US
dc.identifier.endpage627en_US
dc.identifier.issn1536-4798
dc.identifier.issue5en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage621en_US
dc.identifier.urihttps://dx.doi.org/10.1097/ICO.0000000000002229
dc.identifier.urihttps://hdl.handle.net/20.500.12684/269
dc.identifier.volume39en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherNLM (Medline)en_US
dc.relation.ispartofCorneaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.titleEffects of Different Doses of Systemic Isotretinoin on Eyes: A Histopathological and Immunohistochemical Study in Ratsen_US
dc.typeArticleen_US

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