Spinal Kord İskemi-Reperfüzyon Hasarına Montelukastın Etkisi
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Dosyalar
Tarih
2015
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Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
AmAÇ: Çalışma, deneysel bir modelde, seçici tersinir bir CysLT1 reseptör antagonisti olan montelukastın, spinal kord iskemi-reperfüzyon (I/R) hasarı üzerindeki rolünü incelemek için tasarlandı. yÖntem ve GereÇler: Yirmi bir erkek Sprague-Dawley tipi sıçan, rasgele üç gruba (her grupta n 7) ayrıldı; G1 (aort oklüzyonu ve montelukast uygulaması yok), G2 (45 dakika aort oklüzyonu; montelukast uygulaması yok) ve G3 (45 dk aort oklüzyonu, 10 mg / kg montelukast uygulaması).Reperfüzyonun 48. saatindeki Motor Defisit Indeksi (MID) skorlaması kullanılarak yapılan nörolojik değerlendirmeden sonra, lomber spinal kordlar, histopatolojik değerlendirme ve HSP70, interlökin-6 ve miyeloperoksidaz (MPO) için immünhistokimyasal boyama amacıyla çıkartıldı. BulGulAr: G1 grubundaki tüm sıçanların nörolojik durumu normaldi ve MDI skorları 0\'dı (p 0.05). G3\'ün MDI skoru, G2 grubuna göre anlamlı derecede düşük bulundu (2.8 vs 5.5; p 0.05 ). G1'de vaküoler konjesyon, diğer gruplara göre anlamlı derecede düşük bulundu (p 0.0001). İnterlökin-6 reseptörü düzeyi, G3 grubunda kontrol grubuna göre istatistiksel olarak anlamlı düzeyde daha düşük bulundu (p 0.013). HSP70 ve MPO boyanma derecesi açısından gruplar arasında istatistiksel olarak anlamlı fark yoktu. Postiskemik organlarda lökotrienlerin artan üretimi, I/R hasarında önemli bir rol oynamaktadır. sOnuÇ: Çalışmanın bulguları, montelukastın motor iyileşmeyi geliştirdiğini ve spinal kordun I/R hasarında IL-6 düzeylerini azalttığını göstermiştir.
AIm: Paraplegia due to ischemia-reperfusion (I/R) injury of the spinal cord is a devastating complication of thoracoabdominal aortic surgery. Cysteinyl leukotrienes are potent mediators of inflammation that are associated with I/R injury. The present study was designed to investigate the role of montelukast, a selective reversible CysLT1 receptor antagonist, on spinal cord I/R injury in an experimental model. mAterIAl and methOds: Twenty-one male Sprague-Dawley rats were randomly assigned to three groups (n7 per group) as G1 (no aortic occlusion and montelukast administration), G2 (45 min. aortic occlusion; no montelukast administration) and G3 (45 min. aortic occlusion, 10 mg/kg montelukast administration). After neurologic evaluation using the Motor Deficit Index (MDI) score at the 48th hour of reperfusion, lumbar spinal cords were removed for histopathological evaluation and immunohistochemical staining for HSP70, interleukin-6 and myeloperoxidase (MPO). results: All rats in the G1 group had a normal neurological status and their MDI score was 0 (p<0.05). The MDI score of G3 was significantly lower than G2 group (2.8 vs. 5.5; p<0.05). Vacuolar congestion was found to be significantly lower in G1 than the other groups (p0.0001). The interleukin-6 receptor level was found to be significantly lower in G3 group than the control group (p0.013). There was no statistically significant difference found among the groups in terms of the degree of HSP70 and MPO staining. COnClusIOn: Increased generation of leukotrienes in postischemic organs play an important role in I/R injury. The findings of the current study demonstrated that montelukast improved motor recovery and decreased IL-6 levels in spinal cord I/R injury.
AIm: Paraplegia due to ischemia-reperfusion (I/R) injury of the spinal cord is a devastating complication of thoracoabdominal aortic surgery. Cysteinyl leukotrienes are potent mediators of inflammation that are associated with I/R injury. The present study was designed to investigate the role of montelukast, a selective reversible CysLT1 receptor antagonist, on spinal cord I/R injury in an experimental model. mAterIAl and methOds: Twenty-one male Sprague-Dawley rats were randomly assigned to three groups (n7 per group) as G1 (no aortic occlusion and montelukast administration), G2 (45 min. aortic occlusion; no montelukast administration) and G3 (45 min. aortic occlusion, 10 mg/kg montelukast administration). After neurologic evaluation using the Motor Deficit Index (MDI) score at the 48th hour of reperfusion, lumbar spinal cords were removed for histopathological evaluation and immunohistochemical staining for HSP70, interleukin-6 and myeloperoxidase (MPO). results: All rats in the G1 group had a normal neurological status and their MDI score was 0 (p<0.05). The MDI score of G3 was significantly lower than G2 group (2.8 vs. 5.5; p<0.05). Vacuolar congestion was found to be significantly lower in G1 than the other groups (p0.0001). The interleukin-6 receptor level was found to be significantly lower in G3 group than the control group (p0.013). There was no statistically significant difference found among the groups in terms of the degree of HSP70 and MPO staining. COnClusIOn: Increased generation of leukotrienes in postischemic organs play an important role in I/R injury. The findings of the current study demonstrated that montelukast improved motor recovery and decreased IL-6 levels in spinal cord I/R injury.
Açıklama
Anahtar Kelimeler
Cerrahi
Kaynak
Turkish Neurosurgery
WoS Q Değeri
Scopus Q Değeri
Cilt
25
Sayı
5
