Stability of Metarhizium anisopliae (Hypocreales: Clavicipitaceae) isolates during repeated in vitro subculture and evaluation of an oil-in-water mycoinsecticide

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dc.contributor.authorSönmez, Emine
dc.contributor.authorUzunoğlu, Hülya
dc.contributor.authorEski, Ardahan
dc.contributor.authorDemirbag, Zihni
dc.contributor.authorDemir, İsmail
dc.date.accessioned2023-07-26T11:53:51Z
dc.date.available2023-07-26T11:53:51Z
dc.date.issued2022
dc.departmentDÜ, Rektörlük, Arıcılık Araştırma Geliştirme ve Uygulama Merkezien_US
dc.description.abstractNine Metarhizium anisopliae (Hypocreales: Clavicipitaceae) isolates were evaluated for efficacy against Melolontha melolontha (Coleoptera: Scarabaeidae) larvae, stability in culture, and the superior isolate used to produce an oil-in-water mycoinsecticide. The phenotypic and genotypic characters of four isolates with high virulence were evaluated for their stability after repeating 12 cycles of in vitro subculture. Repeated subculture did not affect the germination of conidia; however, the morphology of some isolates changed significantly. Three isolates lost their virulence, whereas the KTU-2 isolate remained highly pathogenic. Therefore, KTU-2 was selected as the superior isolate for mycoinsecticide production. After the conidia of KTU-2 were produced by solid-state fermentation using cracked rice as the substrate, the conidia were formulated as an oil-in-water emulsion and its efficacy was assessed. The formulation caused 80% mortality on Me. melolontha larvae even at the lowest application rate (1 x 10(5) conidia/mL) in pot experiments, and complete mortality was obtained with the concentration of 1 x 10(7) conidia/mL. Lethal concentrations that kill 50% and 95% of Me. melolontha larvae present were estimated as 9.29 x 10(3) and 2.1 x 10(6) conidia/mL, respectively. Oil-in-water mycoinsecticide could be a potential candidate for the commercial control of Me. melolontha and other white grubs.en_US
dc.description.sponsorshipKaradeniz Technical University [FBA-6915]en_US
dc.description.sponsorshipThe authors thank the Karadeniz Technical University (Project number: FBA-6915) for financial support and Dr. I.T. Riley for advice during preparation of the manuscript.en_US
dc.identifier.doi10.4039/tce.2022.13
dc.identifier.issn0008-347X
dc.identifier.issn1918-3240
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-85131321139en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.urihttps://doi.org/10.4039/tce.2022.13
dc.identifier.urihttps://hdl.handle.net/20.500.12684/12629
dc.identifier.volume154en_US
dc.identifier.wosWOS:000799346300001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorSönmez, Emine
dc.language.isoenen_US
dc.publisherCambridge Univ Pressen_US
dc.relation.ispartofCanadian Entomologisten_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmz$2023V1Guncelleme$en_US
dc.subjectEntomopathogenic Fungus; Relative-Humidity; Pr1 Activity; Shelf-Life; Virulence; Growth; Conidia; Germination; Tolerance; Nutritionen_US
dc.titleStability of Metarhizium anisopliae (Hypocreales: Clavicipitaceae) isolates during repeated in vitro subculture and evaluation of an oil-in-water mycoinsecticideen_US
dc.typeArticleen_US

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