Effect of process variables on in vitro characteristics of clindamycin phosphate loaded PLGA nanoparticles in dental bone regeneration and 3D characterization studies using nano-CT

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dc.authoridİlhan, Miray/0000-0003-1749-5129
dc.authoridOrhan, Kaan/0000-0001-6768-0176
dc.authoridKılıçarslan, Müge/0000-0003-3710-7445
dc.authorwosidİlhan, Miray/GQZ-8312-2022
dc.authorwosidKılıçarslan, Müge/AAG-1353-2020
dc.contributor.authorİlhan, Miray
dc.contributor.authorKılıçarslan, Müge
dc.contributor.authorOrhan, Kaan
dc.date.accessioned2023-07-26T11:57:27Z
dc.date.available2023-07-26T11:57:27Z
dc.date.issued2022
dc.departmentDÜ, Eczacılık Fakültesi, Eczacılık Teknolojisi Bölümüen_US
dc.description.abstractBone tissue surrounding the teeth may be lost due to traumatic, pathological, or physiological reasons, which can result in tooth loss. Therefore, studies in the field of dental surgery have focused on preventing bone loss around the teeth as well as bone regeneration. In the present work, clindamycin phosphate loaded polymeric nano -particles were developed as a local drug delivery system to promote alveolar bone regeneration. The effects of PVA concentration, drug/polymer ratio, inner phase content, polymer type, and pH of the external water phase on the characterization of nanoparticles were investigated by determining the encapsulation efficiency, particle size and size distribution, surface morphology, 3D structure, drug release, and release kinetics of formulations. Encapsulation efficiency was highly dependent on PVA concentration and pH of the external water phase. The mean particle size decreased with the decrease in molecular weight of PLGA. Although using clindamycin phosphate, a water soluble drug, sustained release was achieved for up to 3 months for all formulations. This study revealed that nano-CT visualization is critical in explaining the effects of process parameters on the characterization of nanoparticles. With the 3D images obtained through nano-CT, the internal and external structural properties of the nanoparticles were obtained both visually and quantitatively. The findings of nano -CT imaging that simultaneously determine the 3D structure, size, volume and porosity of nanoparticles were promising for further studies unlike SEM. The differences of internal structural properties between the nano -particles prepared using PCL and PLGA, which could not be distinguished using SEM images, were revealed through nano-CT imagery. Thus, thanks to the 2D and 3D images obtained by nano-CT, it was determined that densely capsular nanoparticles were formed using PCL, and nanoparticles in a matrix structure were formed with PLGA. In addition, the use of nano-CT scanning was particularly effective in interpreting the dissolution rate. Imaging the internal structures of the nanoparticles gave interpretable results in the formulation choices made in this study.en_US
dc.description.sponsorshipAnkara University, Coordinator of Scientific Research Projects, Turkey [17A0234001]en_US
dc.description.sponsorshipThis research was supported by Ankara University, Coordinator of Scientific Research Projects, Turkey (Grant number: 17A0234001) .en_US
dc.identifier.doi10.1016/j.jddst.2022.103710
dc.identifier.issn1773-2247
dc.identifier.issn2588-8943
dc.identifier.scopus2-s2.0-85137117692en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1016/j.jddst.2022.103710
dc.identifier.urihttps://hdl.handle.net/20.500.12684/13184
dc.identifier.volume76en_US
dc.identifier.wosWOS:000860350500007en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorİlhan, Miray
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofJournal of Drug Delivery Science and Technologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmz$2023V1Guncelleme$en_US
dc.subjectClindamycin Phosphate; Nanoparticle; Bone Regeneration; Plga; Nano-Ct; Periodontal Drug Deliveryen_US
dc.subjectVivo Evaluation; Delivery; Drug; Release; Microparticles; Microspheres; Scaffolds; Formulation; Pclen_US
dc.titleEffect of process variables on in vitro characteristics of clindamycin phosphate loaded PLGA nanoparticles in dental bone regeneration and 3D characterization studies using nano-CTen_US
dc.typeArticleen_US

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