Characterization of cellulase by Cellvibrio polysaccharolyticus and assessment of its application in the textile industry

Basit Öğe Kaydı
dc.contributor.authorKizmaz, Kerem
dc.contributor.authorEmire, Zuhal
dc.contributor.authorUgras, Serpil
dc.date.accessioned2025-10-11T20:48:23Z
dc.date.available2025-10-11T20:48:23Z
dc.date.issued2025
dc.departmentDüzce Üniversitesien_US
dc.description.abstractThere are many enzymes used in various industries, and the application areas of enzymes are increasing day by day. Among these enzymes, cellulase enzymes, which are of industrial importance, are used in many industrial sectors such as paper pulp, textiles, and food. In this study, bacteria isolated from bovine feces that produce cellulase enzyme were screened. Then, a bacterium identified as a producer, coded as UR4. The isolated bacterium was identified as Cellvibrio polysaccharolyticus (Cp-UR4). Thus, for the first time in the literature, the Cp-UR4 was used for cellulase production in this study. The optimum production conditions of the enzyme were determined to be 37 degrees C, pH 7.0, and 72 h of incubation. The enzyme working conditions were determined to be at 45 degrees C and pH 7.0. The Vmax and Km values for enzyme kinetics were found to be 6.41 U/mL and 12.53 mM, respectively. Ammonium sulfate method was used for partial purification of the enzyme in the final stage. Native-PAGE and zymogram analysis were performed to determine the molecular weight of the partially purified enzyme. It was determined that the cellulase formed aggregates larger than 130 kDa. The enzyme was applied to denim fabric, and the results showed that it performed better in de-fuzzing, bio-polishing, and color removal compared to the reference enzyme. Thus, this study suggests that cellulase obtained for the first time from Cp-UR4, isolated from bovine feces, can be used for bio-polishing, color removal, and de-fuzzing processes in the textile industry.en_US
dc.identifier.doi10.1080/00405000.2025.2518732
dc.identifier.issn0040-5000
dc.identifier.issn1754-2340
dc.identifier.scopus2-s2.0-105008952288en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1080/00405000.2025.2518732
dc.identifier.urihttps://hdl.handle.net/20.500.12684/21891
dc.identifier.wosWOS:001513350200001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Ltden_US
dc.relation.ispartofJournal of the Textile Instituteen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmzKA_WOS_20250911
dc.subjectCellulaseen_US
dc.subjectCellvibrio polysaccharolyticusen_US
dc.subjectDenim fabricen_US
dc.subjectOptimizationen_US
dc.subjectZymogram analysisen_US
dc.titleCharacterization of cellulase by Cellvibrio polysaccharolyticus and assessment of its application in the textile industryen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu