The effect of etanercept on Spinal epidural Fibrosis in a Postlaminectomy Rat Model
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Dosyalar
Tarih
2014
Dergi Başlığı
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Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
AMAÇ: Posterior spinal cerrahi sonrası duramatere yapışık epidural fibrozis formasyonu vücudun cerrahiye karşı vermiş olduğu normal bir reaksiyondur. Aşırı epidural fibrozis postlaminektomi sendromuna yol açan önemli nedenlerden biridir. Etanersept tümör nekrozis faktör alfayı inhibe ederek fibroblast migrasyonunu engeller ve sonuç olarak fibrozis gelişmesini önler. Bu çalışmanın amacı; topikal uygulanan etanerseptin rat laminektomi modelinde gelişen epidural fibrozis üzerine etkisini araştırmaktır. YÖNTEM ve GEREÇLER: 24 Wistar rat rastgele ve eşit olarak üç gruba (Kontrol, spongostan ve etanersept) ayrıldı. Bütün ratlara L3-L5 laminektomi yapıldı. Spongostana emdirilmiş salin (0.1mg/kg) ve etanersept (300 µg/kg) direkt duramatere uygulandı ve duramater üzerinde bırakıldı. 4 hafta sonra ratların T10-L5 arası omurgaları enblok olarak çıkartılarak, epidural fibrozis ve araknoidal tutulum histopatolojik olarak incelendi ve derecelendirildi. BULGULAR: Kontrol grubu ile karşılaştırıldığında topikal uygulanan etanersept grubunda epidural fibrozisin istatistiksel olarak anlamlı derecede azaldığı görüldü (p0.05). SONUÇ: Çalışmamız; topikal uygulanan etanerseptin laminektomi yapılmış ratlarda gelişen epidural fibrozisin azaltılmasında etkili olduğunu göstermiştir.
AIM: The formation of epidural fibrosis adjacent to the dura mater after posterior spinal surgery is a normal reaction of the body to surgery. Extensive epidural fibrosis is one of the important causes of postlaminectomy syndrome. Etanercept inhibits tumor necrosis factor-alpha and decreases fibroblast migration. Thus, etanercept prevents the formation of fibrosis. The aim of this study was to investigate the effects of topical application of etanercept on epidural fibrosis after laminectomy in a rat model. MATERIAL and METHODS: Twenty-four Wistar rats were equally and randomly divided into three groups (control, spongostan and etanercept). Laminectomy was performed between L3 and L5 in all the rats. Spongostan soaked with saline (0.1 mg/kg) and etanercept (300 µg/kg) was directly exposed to and left on the dura mater. Four weeks later, the vertebral columns of the rats were removed en bloc between T10 and L5, and epidural fibrosis and arachnoidal involvement were evaluated and graded histopathologically. RESULTS: Our data revealed that epidural fibrosis was reduced significantly in the rats treated with etanercept, compared to the control groups (p<0.05). CONCLUSION: Our study demonstrated that topical application of etanercept can be effective in reducing epidural fibrosis in rats after laminectomy.
AIM: The formation of epidural fibrosis adjacent to the dura mater after posterior spinal surgery is a normal reaction of the body to surgery. Extensive epidural fibrosis is one of the important causes of postlaminectomy syndrome. Etanercept inhibits tumor necrosis factor-alpha and decreases fibroblast migration. Thus, etanercept prevents the formation of fibrosis. The aim of this study was to investigate the effects of topical application of etanercept on epidural fibrosis after laminectomy in a rat model. MATERIAL and METHODS: Twenty-four Wistar rats were equally and randomly divided into three groups (control, spongostan and etanercept). Laminectomy was performed between L3 and L5 in all the rats. Spongostan soaked with saline (0.1 mg/kg) and etanercept (300 µg/kg) was directly exposed to and left on the dura mater. Four weeks later, the vertebral columns of the rats were removed en bloc between T10 and L5, and epidural fibrosis and arachnoidal involvement were evaluated and graded histopathologically. RESULTS: Our data revealed that epidural fibrosis was reduced significantly in the rats treated with etanercept, compared to the control groups (p<0.05). CONCLUSION: Our study demonstrated that topical application of etanercept can be effective in reducing epidural fibrosis in rats after laminectomy.
Açıklama
Anahtar Kelimeler
Cerrahi
Kaynak
Turkish Neurosurgery
WoS Q Değeri
Scopus Q Değeri
Cilt
24
Sayı
4
