Induction of Autophagic Cell Death by Thymoquinone in Docetaxel Resistant Prostate Cancer Cells

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dc.contributor.authorOguz, Ferdi
dc.contributor.authorİlhan, Suleyman
dc.date.accessioned2023-04-10T20:20:56Z
dc.date.available2023-04-10T20:20:56Z
dc.date.issued2021
dc.departmentRektörlük, Rektörlüğe Bağlı Birimler, Düzce Üniversitesi Dergilerien_US
dc.description.abstractAim: Acquired docetaxel (DOC) resistance of prostate cancer (PCa) is still a clinical problem. In addition to failure in chemotherapy treatment, it causes tumor recurrence. Therefore, novel and more effective compounds are needed in DOC-resistant PCa treatment. This study aimed to investigate the possible cytotoxic and cell death-inducing activities of thymoquinone (TQ), one of the main active components of Nigella sativa L., on DOC-resistant prostate cancer cells. Material and Methods: DOC-resistant PC3 cells (DOC-R/PC3) were developed by the continuous culture with increment concentrations of DOC (1-10 nM) until they improved their growth and division abilities. The cell viability was determined by MTT assay. The MuseTM Annexin V & Dead Cell kit was performed to detect apoptotic cell death. Autophagic vacuoles were observed by staining autophagic vacuoles. The levels of LC3I, LC3II and Beclin-1 proteins were investigated via western blot analysis. Results: TQ inhibited the viability of DOC-R/PC3 cells in a dose- and time-dependent manner (p=0.014). The IC50 value of TQ for DOC-R/PC3 cells was calculated as 60 µM at 72 h. Treatment of TQ did not induce apoptotic cell death in DOC-resistant prostate cancer cells but induced the formation of autophagic vacuoles. Moreover, Beclin-1 and LC3-II protein levels were increased in TQ-treated DOC-R/PC3 cells, however, LC3-I levels were decreased in DOC-R/PC3 cells. Conclusion: All these results show that TQ may become a new therapeutic target for DOC-resistant prostate cancer in the future.en_US
dc.identifier.doi10.18678/dtfd.925238
dc.identifier.endpage191en_US
dc.identifier.issn1307-671X
dc.identifier.issue2en_US
dc.identifier.startpage187en_US
dc.identifier.trdizinid498453en_US
dc.identifier.urihttp://doi.org/10.18678/dtfd.925238
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/498453
dc.identifier.urihttps://hdl.handle.net/20.500.12684/11480
dc.identifier.volume23en_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.relation.ispartofDüzce Tıp Fakültesi Dergisi
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectLC3en_US
dc.subjectautophagyen_US
dc.subjectBeclin-1en_US
dc.subjectresistanceen_US
dc.subjectprostate canceren_US
dc.subjectthymoquinoneen_US
dc.titleInduction of Autophagic Cell Death by Thymoquinone in Docetaxel Resistant Prostate Cancer Cellsen_US
dc.typeArticleen_US

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