Identification of the Gram Positive Bacterial Sepsis Agent with Rapid Genotype Test

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dc.contributor.authorErdogan, Ziya
dc.contributor.authorOzturk, Cihadiye Elif
dc.contributor.authorCangur, Sengul
dc.contributor.authorCaliskan, Emel
dc.contributor.authorOksuz, Sukru
dc.contributor.authorKaramurat, Zeynep Dilara
dc.date.accessioned2021-12-01T18:47:26Z
dc.date.available2021-12-01T18:47:26Z
dc.date.issued2021
dc.department[Belirlenecek]en_US
dc.description.abstractObjective: An irreversible process begins when a systemic infection causes sepsis. Therefore, rapid identification of the agent bacteria in sepsis and its antibiotic resistance is crucially important. In this study, it was aimed to investigate the efficiency of rapid genotype test in detecting sepsis agent Gram positive bacteria and important antibiotic resistance. Methods: 2132 blood culture samples sent to the laboratory were examined with an automatic blood culture system (BACTEC, BD, USA) between 2018-2019. Blood culture bottles sent to the laboratory were Growing bacteria was identificated by VITEK (bioMerieux, France) automated bacteria identification / antibiotic susceptibility system. In addition, bacterial species and mecA, vanA, vanB, vanC1, vanC2 / C3 genes in blood cultures with Gram positive bacterial growth were also determined by the Genotype (R) BC Gram-positive (Hain Lifesience, Germany) test. Results: 72 patients with gram-positive bacteria growth in two or more blood culture bottles were included in the study. In 44 of the samples (61%) the same bacterial species were detected with conventional method (bacteria culture) and BC Gram positive test. In 28 of the samples (39%) differences were detected between results of methods regarding bacterial species name or vancomycin/methicillin resistance rate. Although single agent was isolated with culture method in all of the samples, multiple agents were detected in eight samples with rapid genotype test. Also, it was found that in mecA positive samples, ciprofloxacin resistance was higher than mecA negative ones. Conclusions: In the study, it was observed that BC Gram positive test could correctly identify sepsis agent bacteria and their resistance genes within 4-5 hours.en_US
dc.description.sponsorshipDuzce University Scientific Research Projects CoordinationDuzce University [017.04.01.618]en_US
dc.description.sponsorshipThis research was supported by Duzce University Scientific Research Projects Coordination; with the project title Identification and resistance determination in blood cultures with a growth of gram positive bacteria with genotyping, number 017.04.01.618.en_US
dc.identifier.doi10.18521/ktd.834769
dc.identifier.endpage518en_US
dc.identifier.issn1309-3878
dc.identifier.issue3en_US
dc.identifier.startpage511en_US
dc.identifier.urihttps://doi.org/10.18521/ktd.834769
dc.identifier.urihttps://hdl.handle.net/20.500.12684/10271
dc.identifier.volume13en_US
dc.identifier.wosWOS:000709040200004en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherDuzce Univ, Fac Medicineen_US
dc.relation.ispartofKonuralp Tip Dergisien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectDrug Resistanceen_US
dc.subjectPCRen_US
dc.subjectRapid testen_US
dc.subjectSepsisen_US
dc.subjectStaphylococcien_US
dc.subjectResistant Staphylococcus-Aureusen_US
dc.subjectBlood Culture Bottlesen_US
dc.subjectTime Pcr Assayen_US
dc.subjectVan Genesen_US
dc.subjectMecaen_US
dc.subjectInfectionsen_US
dc.subjectPathogensen_US
dc.titleIdentification of the Gram Positive Bacterial Sepsis Agent with Rapid Genotype Testen_US
dc.typeArticleen_US

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