Determination of Antioxidant and Anticancer Activities of C. Sativa Leaf Extracts on MCF7 Human Breast Cancer Cell Line

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dc.contributor.authorBeyazyüz, Fadime
dc.contributor.authorMutlu, Elif Gülbahçe
dc.contributor.authorAlpa, Serife
dc.contributor.authorErbayram, Fatma Zehra
dc.contributor.authorTürkoğlu, Fatma Nur
dc.contributor.authorKulaç, Şemsettin
dc.date.accessioned2025-03-24T19:48:33Z
dc.date.available2025-03-24T19:48:33Z
dc.date.issued2023
dc.departmentDüzce Üniversitesi
dc.description.abstractAim: In this study, it was aimed to determine the total phenolic content, flavonoid content, and antioxidant activity of Castanea sativa Mill. (chestnut) leaf extract and their anti-proliferative effect on MCF 7 cell line. Materials and Methods: It determined antioxidant capacities by using DPPH assay, total phenolic content by using Folin-Ciocaltaeu method and Total flavonoid content by aluminum chloride colorimetric method in the ethanolic extracts of Chesnut leaf. In addition, the anti-proliferative effect of chestnut leaf extract was determined by XTT method in MCF7 breast cell line. The leaf extract used was applied at different concentrations for 24 hours, 48 hours and 72 hours and the results were evaluated with the Graphpad Prism software program. Results: In this study, it was revealed that the total phenolic contents of ethanolic extracts of chestnut leaf 58,22 mg GAE/g. Total flavonoid content was 64,62 mg QE/g. The DPPH activity of the leaf extract of chestnut was 80.06%. Moreover, findings showed that Chestnut leaf extract had cytotoxic effect against breast cancer cells depends on concentration and time. The 24h, 48h and 72h most effective IC50 dose were 100.1 µL ,193 µL 15.23 µL, respectively. Conclusion: Chestnut has potential antiproliferative and antioxidant activities in conjunction with its high phenolic and flavonoid content on the MCF-7 cell line. However, more studies are needed.
dc.identifier.doi10.37990/medr.1276955
dc.identifier.endpage7
dc.identifier.issn2687-4555
dc.identifier.issue3
dc.identifier.startpage472
dc.identifier.urihttps://doi.org/10.37990/medr.1276955
dc.identifier.urihttps://hdl.handle.net/20.500.12684/19050
dc.identifier.volume5
dc.language.isoen
dc.publisherTıbbi Kayıtlar Derneği
dc.relation.ispartofMedical Records
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_DergiPark_20250324
dc.subjectAntioxidant properties|anticancer|chestnut|C. sativa|MCF-7.
dc.titleDetermination of Antioxidant and Anticancer Activities of C. Sativa Leaf Extracts on MCF7 Human Breast Cancer Cell Line
dc.typeArticle

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