Molecular Characterization of Prunus spinosa L. (Rosaceae) Populations from theWest Black Sea Region in Turkey Using Inter-simple Sequence Repeat Polymerase Chain Reaction

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dc.authoridFiliz, Ertugrul/0000-0001-9636-6389;en_US
dc.authorscopusid58557166200en_US
dc.authorscopusid57041190400en_US
dc.authorscopusid57907179800en_US
dc.authorscopusid57896208300en_US
dc.authorscopusid23984566200en_US
dc.authorwosidFiliz, Ertugrul/I-9440-2016en_US
dc.authorwosidSevindik, Emre/M-2621-2019en_US
dc.contributor.authorGulay, Muhammed
dc.contributor.authorSevindik, Emre
dc.contributor.authorSofyalioglu, Erengul
dc.contributor.authorCayir, Muhammed Ebrar
dc.contributor.authorFiliz, Ertugrul
dc.date.accessioned2024-08-23T16:07:11Z
dc.date.available2024-08-23T16:07:11Z
dc.date.issued2023en_US
dc.departmentDüzce Üniversitesien_US
dc.description.abstractIn this study, molecular characterization of four Prunus spinosa populations was carried out using the inter-simple sequence repeat polymerase chain reaction (ISSR-PCR) marker method. Populations were collected from Bolu, Duzce and Zonguldak provinces in Turkey. After genomic DNA (gDNA) isolation from the populations, PCR amplification was performed using 13 ISSR primers. As a result of the study, 116 polymorphic bands were obtained and the polymorphism rate was determined as 81.89%. These results were based on the genetic distance matrix obtained in the NTSYS-pc package version 2.10. The results showed that the closest genotypes were DY2 and DY4, with a similarity value of 0.2037 between them. DY4 and BM1 are found to have a similarity value of 0.5641, which indicates that they are the two genotypes that differ most from one another. The mean polymorphism information content (PIC) value was determined as 0.3507 and the mean Nei's gene diversity (H) value was determined as 0.4559. In the unweighted pair group method with arithmetic mean (UPGMA) dendrogram, 13 populations were divided into two groups. According to principal component analysis (PCA), the first three eigen values explained 55% of the total variance in the population. As a result, the polymorphism rate was found to be very high with ISSR analysis, and the UPGMA dendrogram was compatible with the geographical distribution. The results of studies on P. spinosa populations using the ISSR analysis can be beneficial for cultivar development and further studies in the future.en_US
dc.identifier.doi10.1007/s10341-023-00939-z
dc.identifier.issn0014-0309
dc.identifier.issn1439-0302
dc.identifier.scopus2-s2.0-85169337239en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.urihttps://doi.org/10.1007/s10341-023-00939-z
dc.identifier.urihttps://hdl.handle.net/20.500.12684/14537
dc.identifier.wosWOS:001065251000001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofErwerbs-Obstbauen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectGenetic diversityen_US
dc.subjectMolecular markeren_US
dc.subjectPhylogeneticsen_US
dc.subjectPlumen_US
dc.subjectTurkeyen_US
dc.subjectGenetic Diversityen_US
dc.subjectPhenolic-Compoundsen_US
dc.subjectRelatednessen_US
dc.subjectPhylogenyen_US
dc.subjectFruitsen_US
dc.subjectRapden_US
dc.titleMolecular Characterization of Prunus spinosa L. (Rosaceae) Populations from theWest Black Sea Region in Turkey Using Inter-simple Sequence Repeat Polymerase Chain Reactionen_US
dc.typeArticleen_US

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