Selenyum Dioksit ve Paklitaksel Kombinasyonunun MDA-MB-231 Meme Kanseri Hücre Hattı Üzerine Etkisinin Değerlendirilmesi
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Dosyalar
Tarih
2023
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Düzce Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Bu çalışmanın amacı, bitkisel kökenli bileşik olan selenyum dioksit (SEL) ile kemoterapötik ilaç olan paklitaksel’in (PTX) kombine uygulamasının MDA-MB-231 meme kanseri hücre hattı üzerine etkisinin belirlenmesidir.Gereç ve Yöntemler: Çalışmamızda öncelikle SEL’in yarı maksimal inhibitör konsantrasyonunun belirlenmesi amacıyla MTT testi yapıldı. Deney grupları SEL1, SEL2, SEL3, PTX, SEL1+PTX, SEL2+PTX ve SEL3+PTX şeklinde oluşturulduktan sonra apoptoz TUNEL metodu, NF-kB ekspresyonu immunofloresan boyama metodu ile hücre canlılığı ise MTT analizi ile gösterildi. Bulgular: SEL’in meme kanseri hücrelerinde hücre canlılığını azalttığı gösterildi. SEL1, SEL2, SEL3, PTX gruplarında kontrole göre apoptozun arttığı görüldü (p0,999, p>0,999, p=0,725).Sonuç: Mevcut çalışmada MDA-MB-231 meme kanseri hücre dizisinde SEL ve PTX’in antagonistik etkiye sahip oldukları belirlenmiştir.
Aim: The aim of this study is to determine the effect of the combined application of the plant-derived compound selenium dioxide and the chemotherapeutic drug paclitaxel (PTX) on the MDA-MB-231 breast cancer cell line.Material and methods: In our study, first of all, the MTT test was performed to determine the semi-maximal inhibitory concentration of selenium dioxide. After the experimental groups were formed as SEL1, SEL2, SEL3, PTX, SEL1+PTX, SEL2+PTX, and SEL3+PTX, apoptosis was demonstrated by the TUNEL method, NF-kB expression was demonstrated by the immunofluorescent staining method, and cell viability was demonstrated by MTT analysis.Results: Selenium dioxide has been shown to decrease cell viability in breast cancer cells. Apoptosis was observed to be increased in SEL1, SEL2, SEL3, and PTX groups compared to control(p
Aim: The aim of this study is to determine the effect of the combined application of the plant-derived compound selenium dioxide and the chemotherapeutic drug paclitaxel (PTX) on the MDA-MB-231 breast cancer cell line.Material and methods: In our study, first of all, the MTT test was performed to determine the semi-maximal inhibitory concentration of selenium dioxide. After the experimental groups were formed as SEL1, SEL2, SEL3, PTX, SEL1+PTX, SEL2+PTX, and SEL3+PTX, apoptosis was demonstrated by the TUNEL method, NF-kB expression was demonstrated by the immunofluorescent staining method, and cell viability was demonstrated by MTT analysis.Results: Selenium dioxide has been shown to decrease cell viability in breast cancer cells. Apoptosis was observed to be increased in SEL1, SEL2, SEL3, and PTX groups compared to control(p
Açıklama
Anahtar Kelimeler
apoptoz, MDA-MB-231, NF-k?, Paklitaksel, Selenyum dioksit, Apoptosis, MDA-MB-231, NF-k?, Paclitaxel, Selenium dioxide
Kaynak
Sağlık Bilimlerinde Değer
WoS Q Değeri
Scopus Q Değeri
Cilt
13
Sayı
2
