Determination of Cellulase Enzyme Produced by Bacillus cereus DU-1 Isolated from Soil, and Its Effects on Cotton Fiber

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dc.authoridugras, serpil/0000-0002-1867-5781en_US
dc.authorscopusid54397548800en_US
dc.authorscopusid59193132000en_US
dc.authorscopusid57193210810en_US
dc.authorwosidugras, serpil/KSM-3256-2024en_US
dc.contributor.authorUgras, Serpil
dc.contributor.authorBicen, Hatice Elif Isik
dc.contributor.authorEmire, Zuhal
dc.date.accessioned2024-08-23T16:03:55Z
dc.date.available2024-08-23T16:03:55Z
dc.date.issued2024en_US
dc.departmentDüzce Üniversitesien_US
dc.description.abstractA Cellulase enzyme is the enzyme synthesized by some bacterial and fungal species and breaks down the ss-1,4 glycoside bonds of cellulose. Cellulase enzyme has a wide range of uses such as food, paper, textile industry, animal feed and detergent production. In this study, which was designed to add a new cellulase enzyme with such a wide industrial use, cellulotic bacterium isolation, identification, partial purification, characterization and application on denim fabric of the cellulase enzyme synthesized by the identified bacterium was performed. Firstly, bacterium that can produce cellulase enzyme was isolated from soil samples containing hazelnut wastes. The enzyme-producing isolate was identified as Bacillus cereus strain DU -1 by morphological, biochemical and molecular techniques. The optimum conditions for enzyme production by B. cereus DU -1 was determined as pH 7.0, at 37 degrees C and 24h. However, the activity of the enzyme (5.16 U/mL) was found to be optimum at 50 degrees C and pH 6.0. The Vmax value of the enzyme was 3.18 U/mL and the Km value was 0.0019 mM. The enzyme was partially purified by ammonium sulfate precipitation. The molecular size of the partially purified enzyme was determined as approximately 40 kDa by SDS-PAGE and Zymogram analysis. Finally, enzyme was applied on the denim fabric and when the fabrics were viewed under the electron microscope, it was seen that they were suitable for textile use.en_US
dc.description.sponsorship[DU.BAP-2021.05.01.1150]en_US
dc.description.sponsorshipThis study was supported by the Scientific Research Project numbered DU.BAP-2021.05.01.1150.en_US
dc.identifier.doi10.1590/1678-4324-2024230391
dc.identifier.issn1516-8913
dc.identifier.issn1678-4324
dc.identifier.scopus2-s2.0-85196966605en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1590/1678-4324-2024230391
dc.identifier.urihttps://hdl.handle.net/20.500.12684/13983
dc.identifier.volume67en_US
dc.identifier.wosWOS:001246249200001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherInst Tecnologia Paranaen_US
dc.relation.ispartofBrazilian Archives of Biology And Technologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBacillus cereusen_US
dc.subjectCellulaseen_US
dc.subjectCharacterizationen_US
dc.subjectEnzyme kineticsen_US
dc.subjectPurificationen_US
dc.subjectAlkaline Cellulaseen_US
dc.subjectCarboxymethyl Cellulaseen_US
dc.subjectPurificationen_US
dc.subjectStrainen_US
dc.subjectEndoglucanaseen_US
dc.subjectHydrolysisen_US
dc.titleDetermination of Cellulase Enzyme Produced by Bacillus cereus DU-1 Isolated from Soil, and Its Effects on Cotton Fiberen_US
dc.typeArticleen_US

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