Effects of radiofrequency exposure on in vitro blood-brain barrier permeability in the presence of magnetic nanoparticles

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dc.authoridÇakmak, Soner/0000-0003-2245-8322
dc.authoridŞentürk, Fatih/0000-0002-2436-3362
dc.authoridŞentürk, Fatih/0000-0002-2436-3362
dc.authorwosidÇakmak, Soner/I-4478-2015
dc.authorwosidŞentürk, Fatih/AEI-3613-2022
dc.authorwosidŞentürk, Fatih/AHD-0721-2022
dc.authorwosidŞentürk, Fatih/HKE-0978-2023
dc.contributor.authorŞentürk, Fatih
dc.contributor.authorÇakmak, Soner
dc.contributor.authorKoçum, İsmail Cengiz
dc.contributor.authorGümüşderelioğlu, Menemse
dc.contributor.authorÖztürk, Göknur Güler
dc.date.accessioned2023-07-26T11:54:00Z
dc.date.available2023-07-26T11:54:00Z
dc.date.issued2022
dc.departmentDÜ, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Biyofizik Ana Bilim Dalıen_US
dc.description.abstractThe blood-brain barrier (BBB) remains a major obstacle for the delivery of drugs in the treatment of many neurological diseases. In this study, we aimed to investigate the effects of radiofrequency electromagnetic fields (RF-EMFs) on the permeability of an in vitro BBB model under RF exposure alone, or in the presence of nanoparticles (NPs). For this purpose, an in vitro BBB model was established by seeding human umbilical vein endothelial cells (HUVECs) and human glioblastoma cell line (T98G) on the apical and basolateral sides of the transwell membrane, respectively. The integrity of the BBB model was confirmed by measuring transendothelial electrical resistance (TEER), and a fluorescein isothiocyanate (FITC)-dextran permeability assay was performed when the resistance reached 120 U cm(2). After the RFfield exposure (13.56 MHz, 80 W, 10 min), we found that FITC-dextran transported across the in vitro BBB was increased 10-fold compared to FITC-dextran transported without an RF-field. This notable phenomenon, which can be called the burst permeability RF effect (BP-RF), has been proposed for the first time in the literature. Subsequently, the effect of the RF-field on BBB permeability was also investigated in the presence of superparamagnetic iron oxide nanoparticles (SPIONs) and magnetic poly(lactic-co-glycolic acid)-polyethylene glycol (PLGA-b-PEG) nanoparticles (m-PNPs). It was found that the amount of both transported NPs on the basolateral sides increased after exposure to the RF-field. As a result, the RFfield can be applied simultaneously during treatment with clinical agents or nanocarriers, improving the permeability of the BBB, which may contribute to therapeutic efficacy of many drugs that are used in neurological diseases. (c) 2022 Elsevier Inc. All rights reserved.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [SBAG-119S137]en_US
dc.description.sponsorshipThis study was supported by the Scientific and Technological Research Council of Turkey (TUBITAK), Grant no: SBAG-119S137. We are sincerely grateful for the assistance of the Cell and Tissue Engineering Research Group at Hacettepe University, and Dr. Anil Sera Cakmak for the cell culture studies. We are also thankful to Dr. Guldeniz Selmanoglu, Dr. Elif Karacaoglu and Deniz Tulumcuoglu for their support in TEER measurements. We are sincerely grateful to Dr. Cagatay Karaaslan and Dilara Karaguzel for their assistance in fluorescence plate reader measurements.en_US
dc.identifier.doi10.1016/j.bbrc.2022.01.112
dc.identifier.endpage97en_US
dc.identifier.issn0006-291X
dc.identifier.issn1090-2104
dc.identifier.pmid35134610en_US
dc.identifier.startpage91en_US
dc.identifier.urihttps://doi.org/10.1016/j.bbrc.2022.01.112
dc.identifier.urihttps://hdl.handle.net/20.500.12684/12690
dc.identifier.volume597en_US
dc.identifier.wosWOS:000755174800001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorŞentürk, Fatih
dc.language.isoenen_US
dc.publisherAcademic Press Inc Elsevier Scienceen_US
dc.relation.ispartofBiochemical and Biophysical Research Communicationsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmz$2023V1Guncelleme$en_US
dc.subjectBlood-Brain Barrier Permeability; Rf-Field; Fitc-Dextran; Magnetic Nanoparticlesen_US
dc.subjectIron-Oxide Nanoparticlesen_US
dc.titleEffects of radiofrequency exposure on in vitro blood-brain barrier permeability in the presence of magnetic nanoparticlesen_US
dc.typeArticleen_US

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