Farklı uzunluktaki incebarsak rezeksiyonu ile kısa barsak sendromu oluşturulan sıçanlarda enteral glutaminin adaptasyona etkisi
Küçük Resim Yok
Tarih
2006
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Amaç: Bu çalışmada farklı uzunlukta incebarsak rezeksiyonu yapılarak kısa barsak sendromu (KBS) oluşturulan sıçanlarda adaptasyon sürecinde glutaminin etkileri araştırıldı. Gereç ve Yöntem: Çalışmamızda 35 adet sıçan kullanıldı. Grup 1'deki (sham) sıçanlara sadece laparatomi uygulandı. Grup 2 ve grup 3 'teki sıçanlara %65 incebarsak rezeksiyonu, grup 4 ve grup 5'teki sıçanlara %85 incebarsak rezeksiyonu yapılarak KBS oluşturuldu. Grup 3 ve grup 5'teki sıçanlara 2,5 g/kg/gün glutamin oral olarak 21 gün süresince verildi. Sıçanların kilo takibi yapıldı ve incebarsak biyopsileri alındı. Bulgular: KBS oluşturulan sıçanların tümünde 1-6. günden itibaren belirgin olarak ishal ve kilo kaybı gelişti (p 0.05). Grup 3'teki sıçanlar ile grup 2 ve grup 4'teki sıçanlar arasında 18.günden sonra kilo artışı anlamlı idi (p 0.05). Villus yüksekliği, kript derinliği, mukozal kalınlık ve mitoz sayısında grup 3'teki değerler ile diğer grupların değerleri arasında belirgin fark vardı (p 0.05). Sonuç: Daha az incebarsak rezeksiyonu uyguladığımız grup 3 sıçanlarda geriye kalan barsağın ve glutamin tedavisinin olumlu etkilerinin sonucu olarak daha iyi adaptasyon geliştiği inancındayız.
Aim: The purpose of the study was to determine the effect of glutamine on adaptation process in short bowel syndrome (SBS) induced rats through small intestinal resection of varied length. Material and Method: A total of 35 rats were used in this study. Laparotomy alone was performed in group 1 rats (sham). Short bowel syndrome was induced by performing small intestinal resection of 65% in groups 2 and 3, and 85% in groups 4 and 5. Glutamine was orally administered at a dose of 2.5 g/kg/day in groups 3 and 5 for 21 days. The rats were followed-up in terms of weight, and small intestinal biopsies were taken at the end of the study. Results: Significant diarrhea and weight loss developed in SBS induced rats between days 1-6 (p< 0.05). Weight gain after 18th day of glutamine administration was significantly higher in group 3 compared to groups 2 and 4. In terms of villus height, crypt depth, mucosal thickness and mitosis count, there was a significant difference between group 3 and other groups in which resection alone was performed (groups 2 and 4). Conclusions: In group 3 rats undergone less small bowel resection, we think that intestinal adaptation develops better in the remaining bowel due to the effect of glutamine.
Aim: The purpose of the study was to determine the effect of glutamine on adaptation process in short bowel syndrome (SBS) induced rats through small intestinal resection of varied length. Material and Method: A total of 35 rats were used in this study. Laparotomy alone was performed in group 1 rats (sham). Short bowel syndrome was induced by performing small intestinal resection of 65% in groups 2 and 3, and 85% in groups 4 and 5. Glutamine was orally administered at a dose of 2.5 g/kg/day in groups 3 and 5 for 21 days. The rats were followed-up in terms of weight, and small intestinal biopsies were taken at the end of the study. Results: Significant diarrhea and weight loss developed in SBS induced rats between days 1-6 (p< 0.05). Weight gain after 18th day of glutamine administration was significantly higher in group 3 compared to groups 2 and 4. In terms of villus height, crypt depth, mucosal thickness and mitosis count, there was a significant difference between group 3 and other groups in which resection alone was performed (groups 2 and 4). Conclusions: In group 3 rats undergone less small bowel resection, we think that intestinal adaptation develops better in the remaining bowel due to the effect of glutamine.
Açıklama
Anahtar Kelimeler
Cerrahi
Kaynak
Çocuk Cerrahisi Dergisi
WoS Q Değeri
Scopus Q Değeri
Cilt
20
Sayı
2
