Enzyme immobilization on polymeric microspheres containing Schiff base for detection of organophosphate and carbamate insecticides

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dc.authoridHASANOGLU OZKAN, ELVAN/0000-0001-7338-4015
dc.authorwosidSARI, NURSEN/AAA-8328-2021
dc.authorwosidOZKAN, Elvan HASANOGLU/AAJ-2225-2021
dc.contributor.authorNartop, Dilek
dc.contributor.authorYetim, Nurdan Kurnaz
dc.contributor.authorOzkan, Elvan Hasanoglu
dc.contributor.authorSari, Nursen
dc.date.accessioned2021-12-01T18:49:15Z
dc.date.available2021-12-01T18:49:15Z
dc.date.issued2020
dc.department[Belirlenecek]en_US
dc.description.abstractIn this study, polymeric microsphere including Schiff base TrisPS-pyr and TrisPS-pyr-Pt(II) were obtained by condensation reaction for improve the enzymatic properties of acetylcholinesterase (AChE) and detect harmful insects. Structures of the synthesized compounds were characterized by spectroscopic methods such as FT-IR, UV-Vis, SEM-EDX, GPC. Acetylcholinesterase enzyme was immobilized to these new polymeric microspheres and their properties such as activity, reusability and storage stability were investigated. In addition, the qualitative determination of carbamate [2,3-dihydro-2,2-dimethyl-7-benzofuranol N-methylcarbamate (carbofuran), 4-methylthio-3,5-xylyl methylcarbamate (methiocarb) and S-methyl-N- (methylcarbamoyl oxy) thioacetamide (methomyl)] and organophosphate [diethyl (dimethyl thiophosphoryl) succinate (malathion), O, S-dimethyl phosphoramidothioate acetyl (acephate) and chlorpyrifos methyl] insecticides by the immobilized enzyme was performed. The reason for the selection of these pesticides is because they are widely used to combat pests of agricultural products (hazelnut, corn, sugar beet, apple and tobacco) which are grown in our region. (C) 2019 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipDuzce University Research FundDuzce University [2017.07.06.661]en_US
dc.description.sponsorshipThis work was supported by Duzce University Research Fund (Project number: 2017.07.06.661).en_US
dc.identifier.doi10.1016/j.molstruc.2019.127039
dc.identifier.issn0022-2860
dc.identifier.issn1872-8014
dc.identifier.scopus2-s2.0-85072167208en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1016/j.molstruc.2019.127039
dc.identifier.urihttps://hdl.handle.net/20.500.12684/10690
dc.identifier.volume1200en_US
dc.identifier.wosWOS:000496810900008en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofJournal Of Molecular Structureen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectPolymeric microsphereen_US
dc.subjectTris(2-aminoethyl)amine polymer-bound acetylcholinesteraseen_US
dc.subjectInsecticideen_US
dc.subjectAcetylcholinesteraseen_US
dc.subjectBiosensoren_US
dc.subjectPesticidesen_US
dc.titleEnzyme immobilization on polymeric microspheres containing Schiff base for detection of organophosphate and carbamate insecticidesen_US
dc.typeArticleen_US

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