Novel polymeric microspheres: Synthesis, enzyme immobilization, antimutagenic activity, and antimicrobial evaluation against pathogenic microorganisms

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dc.contributor.authorNartop, Dilek
dc.contributor.authorDemirel, Birtane
dc.contributor.authorGüleç, Murat
dc.contributor.authorÖzkan, Elvan Hasanoğlu
dc.contributor.authorYetim, Nurdan Kurnaz
dc.contributor.authorSarı, Nurşen
dc.contributor.authorAğar, Güleray
dc.date.accessioned2020-04-30T13:32:54Z
dc.date.available2020-04-30T13:32:54Z
dc.date.issued2020
dc.departmentDÜ, Teknoloji Fakültesi, Polimer Mühendisliği Bölümüen_US
dc.descriptionPubMed ID: 31851403en_US
dc.description.abstractNew polymeric microspheres containing azomethine (1a-1c and 2a-2c) were synthesized by condensation to compare the enzymatic properties of the enzyme glucose oxidase (GOx) and to investigate antimutagenic and antimicrobial activities. The polymeric microspheres were characterized by elemental analysis, infrared spectra (FT-IR), proton nuclear magnetic resonance spectra, thermal gravimetric analysis, and scanning electron microscopy analysis. The catalytic activity of the glucose oxidase enzyme follows Michaelis-Menten kinetics. Influence of temperature, reusability, and storage capacity of the free and immobilized glucose oxidase enzyme were investigated. It is determined that immobilized enzymes exhibit good storage stability and reusability. After immobilization of GOx in polymeric supports, the thermal stability of the enzyme increased and the maximum reaction rate (Vmax) decreased. The activity of the immobilized enzymes was preserved even after 5 months. The antibacterial and antifungal activity of the polymeric microspheres were evaluated by well-diffusion method against some selected pathogenic microorganisms. The antimutagenic properties of all compounds were also examined against sodium azide in human lymphocyte cells by micronuclei and sister chromatid exchange tests. © 2019 Wiley Periodicals, Inc.en_US
dc.identifier.doi10.1002/jbt.22432en_US
dc.identifier.issn1095-6670
dc.identifier.issue2en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://dx.doi.org/10.1002/jbt.22432
dc.identifier.urihttps://hdl.handle.net/20.500.12684/476
dc.identifier.volume34en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherJohn Wiley and Sons Inc.en_US
dc.relation.ispartofJournal of Biochemical and Molecular Toxicologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectantimicrobial property; antimutagenic effect; glucose oxidase; Polymeric microsphere; Pt4+-azomethineen_US
dc.titleNovel polymeric microspheres: Synthesis, enzyme immobilization, antimutagenic activity, and antimicrobial evaluation against pathogenic microorganismsen_US
dc.typeArticleen_US

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