Therapeutic Treatment with Abdominal Adipose Mesenchymal Cells Does Not Prevent Elastase-Induced Emphysema in Rats

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dc.authorid
dc.contributor.authorGülhan, Pınar Yıldız
dc.contributor.authorEkici, Mehmet Savaş
dc.contributor.authorEkici, Aydanur
dc.contributor.authorNiyaz, Mehmet
dc.contributor.authorGülhan, Muhammet
dc.contributor.authorErcin, Mustafa Emre
dc.contributor.authorAksoy, Nurkan
dc.date.accessioned2021-12-01T18:22:55Z
dc.date.available2021-12-01T18:22:55Z
dc.date.issued2020
dc.department[Belirlenecek]en_US
dc.description.abstractOBJECTIVES: Emphysema and chronic bronchitis have different pathophysiologies but both are significant components of chronic obstructive lung disease (COPD). The levels of Matrix metalloproteinase (MMP)-9 in the bronchoalveloar lavage fluid (BALF) and in serumindicate the presence of emphysema. Intratracheal administration of elastase has been used to create a rat model of emphysema. Adiposetissue-derived mesenchymal stem cells (MSC) have been postulated to prevent or reverse emphysema, however, this has not been examined in the rat model of elastase-induced emphysema.MATERIALS AND METHODS: In this study, 31 Wistar albino rats aged 6–8 weeks and weighing 250–300 g were assessed. On day 1, theanimals were treated intratracheally with 0.5 mL saline (control group, n=10), i.e., 0.5 mL saline solution containing 0.1 IU porcine pancreatic elastase (PPE) (Elastase group, n=12) or PPE plus MSC (Elastase-MSC group, n=9) was adminstered per animal. MSCs suspendedin serum were injected via the caudal vein on day 21. At least 106 cells were injected. All animals were sacrificed on day 42 and theemphysema index (EI) was calculated, along with measuring the BALF and serum MMP-9 concentrations.RESULTS: Porcine pancreatic elastase induced a significant degree of emphysema in the PPE groups as compared to the control group,which was determined by the EI index (p=0.008). This was not reversed by MSC treatment. The EI remained significantly low in comprison with the controls (p=0.001) and measured no different from the Elastase-treated animals. There was no statistically significantdifference between the BALF and serum MMP-9 levels between the control and treatment groups.CONCLUSION: Our findings suggest that therapeutic treatment with adipose tissue-derived MSC in rats has no effect on emphysema oron MMP9 expression, which is a known marker of emphysema.en_US
dc.identifier.doi10.5152/TurkThoracJ.2019.180136
dc.identifier.endpage20en_US
dc.identifier.issn2149-2530
dc.identifier.issue1en_US
dc.identifier.startpage14en_US
dc.identifier.urihttps://doi.org/10.5152/TurkThoracJ.2019.180136
dc.identifier.urihttps://app.trdizin.gov.tr/makale/TXpnME56a3pNdz09
dc.identifier.urihttps://hdl.handle.net/20.500.12684/9597
dc.identifier.volume21en_US
dc.identifier.wosWOS:000514100200003en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.relation.ispartofTurkish Thoracic Journalen_US
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subject[No Keywords]en_US
dc.titleTherapeutic Treatment with Abdominal Adipose Mesenchymal Cells Does Not Prevent Elastase-Induced Emphysema in Ratsen_US
dc.typeArticleen_US

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