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Öğe Assessment of markers expressed in human hair follicles according to different skin regions(Wroclaw Medical Univ, 2018) Yay, Arzu; Göktepe, Özge; Bahadır, Anzel; Özdamar, Saim; Öktem, İbrahim Suat; Çoruh, Atilla; Baran, MünevverBackground. Body region-dependent hair follicle (HF) characteristics are concerned with follicular size and distribution, and have been demonstrated to have characteristics for each region of the body. Objectives. The aim of the present study was to investigate the expression patterns of the markers called cytokeratin 15 (K15), cytokeratin 6 (K6) and monoclonal antibody Ki-67, and also apoptosis in HFs, which can be observed in different parts of the human body. Material and methods. In this study, healthy human HFs were taken by biopsy from 5 various donor sites ofthe human body: the scalp,the leg, the abdomen, the back and waist. HF-containing skin specimens taken using cryosection were stained with hematoxylin & eosin (H&E) and K15, K6, Ki-67 and terminal deoxynucleotidyl transferase-mediated digoxigenin-dNTP nick end-labelling (TUNEL) immunofluorescence staining protocol was performed. Results. Different skin legions from the human body were examined histologically. While the HFs of scalp tissue showed anatomically obvious hair layers, some hair sections from other regions, like the leg, the abdomen, back and waist, were not as distinct as in the scalp region. According to our findings, K15 expression was highest in the scalp. In addition, the immunoreactivity (IR) intensity of K15 was significantly decreased in the HFs on the waist and abdominal regions, compared to the scalp and back regions (p < 0.001). However, the IR intensity of K6 in the scalp region was statistically significantly higher than the IR intensity of K6 in the abdomen region (p <0.05). Moreover, we showed intraepithelial apoptosis and proliferation of keratinocytes in the bulge of HF. In the study, Ki-67-positive and TUNEL-positive cell numbers were not statistically significant (p > 0.05). Conclusions. Our findings are important for further investigation of molecular aspects of the human hair follicle stem cells compartments in health and disease, which might be a promising model for comparative studies with different human diseases.Öğe Determination of the Relationship Between Sperm Morphology and DNA Damage in Different Patient Groups(Wiley, 2017) Caner, Burak Cihad; Yay, Arzu; Cantürk, Fazile; Bahadır, Anzel; Sezer, Gülay…Öğe The Effects of Leptin on Rat Brain Development; An Experimental Study(Springer, 2019) Yay, Arzu; Önder, Gözde Özge; Özdamar, Saim; Bahadır, Anzel; Aytekin, Metin; Baran, Münevver…Öğe Protective effects of curcumin and beta-carotene on cisplatin-induced cardiotoxicity: An experimental rat model(Turkish Soc Cardiology, 2018) Bahadır, Anzel; Ceyhan, Ayşe; Gergin, Özlem Öz; Yalçın, Betül; Ülger, Menekşe; Özyazgan, Tuğçe Merve; Yay, ArzuObjective: Cisplatin (CDDP) has been known to be an effective antineoplastic drug; however, it has a cardiotoxic effect. Curcumin (CMN) and beta-carotene (BC) have been suggested to protect biological systems against CDDP-induced damage. The current study was conducted to evaluate the possible protective roles of CMN and BC on CDDP-induced cardiotoxicity in rat cardiac tissues. Methods: A total of 49 adult female Wistar albino rats were equally divided into seven groups as follows: control (no medication), sesame oil (1 mg/kg), CDDP (single dose injection two times as once a week, 5 mg/kg/week), BC (100 mg/kg), CDDP+BC (pretreated BC for 30 min before CDDP injection), CMN (200 mg/kg), and CDDP+CMN (pretreated CMN for 30 min before CDDP injection). These treatments were applied intraperitoneally for CDDP and with gavage for CMN and BC. The oxidative/antioxidant indicators, inflammatory cytokines, and histopathological alterations were examined. Results: These alterations included a marked increase in malondialdehyde (MDA) level, significant decrease in catalase (CAT) and superoxide dismutase (SOD) activities, and significant elevation of tumor necrosis factor (TNF)-alpha, interleukin (IL)-1 beta, interleukin (IL)-6 in the CDDP group compared with the other groups. Histopathologically, CDDP-induced severe myocardial degenerative changes were observed. However, the CDDP-induced disturbances in the above-mentioned parameters significantly improved by treatment with BC and particularly CMN. Conclusion: This study indicated that CDDP treatment markedly caused cardiotoxicity; however, treatment with CMN or BC ameliorated this cardiotoxicity in rats. Furthermore, these findings revealed that treatment with CMN has a higher cardioprotective effect than that with BC against CDDP-induced cardiotoxicity in rat cardiac tissues.
