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Öğe Antioxidant, Antimicrobial and Phenolic Component Analysis of Some Edge Medicinal Plants(2023) Ulger, Sultan; Uğraş, SerpilObjective: The plants, which grow wild in the Eastern Anatolia Region and are known as kari (Arum elongatum; Ae) and mende (Chaerophyllum macrospermum; Cm), are used for treatment purposes such as pain reliever and wound healing properties. It is aimed to determine some biological properties of these plants, which are also used medicinally. Material-Method: Firstly, hexane (H), ethanol (E) and acetone (A) extracts of dried plants were prepared and antimicrobial activities of extracts against 13 indicator microorganisms were determined by agar well diffusion method. Antioxidant activities were determined by DPPH and ABTS analyses and the total phenolic component contents were determined by the Folin-Ciocalteu method. Results: The maximum extract yield was obtained in the ethanol extracts of the plants (AeE;10.4011% CmE;2.4898%), the minimum extract yield was obtained in the acetone extracts (AeA;0.8422% CmA;0.3510%). The hexane extract of A. elongatum has no inhibition activity against E. faecalis and L. monocytogenes, but it has inhibition activity against other indicator microorganisms. The other extracts of A. elongatum and all extracts of C. macrospermum were found to have inhibitory activity against all indicator microorganisms. Furthermore, the highest phenolic content was calculated as 54.60±0.486 ?g mL-1 (AeE) and the lowest content as 3.47±0.001 ?g mL-1 (CmA). As a result of the DPPH method the highest IC50 was calculated as 32.4140 ?g mL-1 (CmH) and the lowest IC50 as 12.7876 ?g mL-1 (AeA) and ABTS method the highest IC50 was calculated as 10.3203 ?g mL-1 (CmE), and the lowest IC50 as 0.2744 ?g mL-1 (CmA). Conclusion: These results show that the plant extracts have strong antimicrobial activity and antioxidant activity.Öğe Bakteriyosinlerin Sağlık Alanında Kullanımı(2022) Uğraş, Serpil; Fidan, AyşenurMevcut antibiyotiklere dirençli patojenlerin dramatik bir şekilde artışı, artan salgın hastalıklar, üstesinden gelinemeyen nozokomiyal enfeksiyonlar gibi birçok alanda patojenlerle mücadele için alternatif stratejilere gereksinim duyulmaktadır. Bu bağlamda, yapılan çalışmalar, bakteriyosinlerin patojenlerle mücadele de alternatif bir strateji olabileceğini göstermektedir. Bakteriyosinler sağlık alanında antikanser, antiviral, antibiyofilm ve antimikrobiyal etkileri kanıtlanmış olan önemli bakteriyal kaynaklı peptitlerdir. Patojenlerin inhibisyonunu sağlarken canlıya ve çevreye zararsız oluşları bakteriyosinleri daha da önemli hâle getirmektedir. Günümüzde laktik asit bakterilerinin bakteriyosinleri çalışmalarda odak noktası hâline gelmiş olsa da Bacillus sp., Escherichia coli ve Klebsiella pneumoniae gibi diğer üretici bakterilerin bakteriyosinleri de sağlık alanında oldukça önemli sonuçlar vermekte ve bu alanda çalışmalar hızla sürdürülmektedir. Bakteriyosinlerin, patojenler ile mücadele de yeni stratejilerin geliştirilmesinde kilit rol üstlenebileceği öngörülmektedir. Bu derleme çalışmasında, bakteriyosinlerin önemi vurgulanmakta olup, sağlık alanında bakteriyosinlerin kullanım olanaklarını sunan çalışmalara yer verilmiştir.Öğe Biological Activity Analysis of Hazelnut (Corylus avellana L.) Green Shell and Leaf Extracts(Kahramanmaras Sutcu Imam Univ, 2016) Oğuzkan, Sibel Bayıl; Uğraş, Serpil; Can, Merve; Uzun, Ayşe; Ülger, Sultan; Üzmez, Sebnem; Uğraş, Halil İbrahimCorylus avellana L., a nut cultivar indigenous to Black Sea region of Turkey, is able to relese more than 10.000 tons of nut leaf and 300.000 tons of green nut Shell annually. Objective of this study was to determine biologic activities of nut leaf and green Shell. In this study, to attain their extracts, green nut shell and nut leaf were treated with methanol in room temperature for 72 hours. Antibacterial activity of extracted substances were analyzed via eight different bacterial strains according to agar well diffusion method. The DPPH (1,1-diphenyl-2-pikrilhydrazyl) method was used for determining the antiradical activity. In this study, the DNA productive activity of extracts were determined by the DNA of the pBR322 plasmid at the end of exposing them to H2O2 and UV-C. As a result of the study, it was determined that hazelnut green leaf and shell showed antiradical activity and antibacterial activities to lots of bacteria. Hazelnut green shell demonstrated a protective potential on DNA against to UV-C and H2O2, but this activity was not the case for hazelnut green leaf.Öğe Characterization of a newly identified lipase from a lipase-producing bacterium(Higher Education Press, 2016) Uğraş, Serpil; Üzmez, ŞebnemBackground: Lipases differ from one another with respect to certain properties, and such differences can be very important for various industrial applications. Considering the rapidly developing nature of the relevant industries, there is a need for new lipases with characteristics differing from those of existing enzymes. Methods: In this study, a bacterium was isolated from both the surface mucus layer and gills of rainbow trout (Oncorhynchus mykiss) from Giresun, Turkey. The bacterial species was identified based on its morphological and physiochemical properties, and on its 16S rDNA sequence. The qualitative activity of the bacterial lipase was determined on Rhodamine B and Tween-20 agar plates. The lipase was partially purified from the supernatant of bacterial cultures, and then characterized. Results: The bacterial strain was identified as Acinetobacter sp. strain SU15. The enzyme from Asp-SU15 exhibits maximum activity toward p-nitrophenyl dodecanoate (C12) at 40°C and pH 8.0. The specific activity of the lipase was calculated to be 10.059 U·L–1. The molecular mass of the enzyme was determined to be ~62 kDa via SDS-PAGE. However, native-PAGE indicated that the enzyme forms very large active aggregates, with molecular masses exceeding 250 kDa. The catalytic activity of the enzyme is enhanced in the presence of Co2+, Ca2+, and methanol, but is partially inhibited by Ni2+, ethyl acetate, and butanol. Conclusions: Further research could examine possible industrial applications for the lipase from Asp-SU15. © 2016, Higher Education Press and Springer-Verlag Berlin Heidelberg.Öğe Characterization of a Thermophilic Lipase from Bacillus licheniformis Ht7 Isolated from Hayran Thermal Springs in Giresun(Ars Docendi, 2017) Uğraş, SerpilIn this study five thermotolerant bacteria were isolated from water and soil of the Hayran thermal springs in Giresun. These bacteria were identified as four Bacillus licheniformis (Bl-Ht1, Bl-Ht2, Bl-Ht5, and Bl-Ht7) and a Bacillus subtilis (Bs-Ht9) based on morphological, physiological, and biochemical characteristics, as well as phylogenetic analysis using 16S rDNA sequences. The strain Bl-Ht7 was exhibited the highest lipase activity, and the specific lipase activity was calculated as 15.916 U g(-1). The maximum enzyme activity was exhibited at 90 degrees C and pH 9.0. The molecular weight was determined as approximate 22 kDa and it was confirmed using the zymogram analysis. The lipase activity was enhanced by the application of Ca2+, C (2+), Zn2+ ions, and some organic solvents but no important differences with the other applicatio ns. The optimal production time and suitable carbon source for the production of lipase were found to 24 h, and olive oil, respectively. The findings show that the temperature of the maximum enzyme activity is higher than the other known Bacillus lipases. The enzyme exhibited tolerance to high temperature, organic solvents, and metal ions, so it is attractive for industrial applications.Öğe Determination of antibacterial activity of resveratrol on some pathogenic bacteria(Avrasya Araştırma Geliştirme Bilim ve Teknoloji Merkezi Limited Şirketi, 2024) Mısır, Nihal; Uğraş, SerpilThis study aims to investigate the antibacterial effect of resveratrol, a natural polyphenol, on some pathogenic bacteria. Resveratrol is a compound found in plants such as red grapes and is known for its antioxidant, anti-inflammatory and antimicrobial properties. Due to these properties, it is considered as a potential agent in the prevention and treatment of bacterial infections. In this context, in this study, it was aimed to determine the antibacterial effects and effective dosage of resveratrol on Salmonella typhimurium, Staphylococcus epidermidis, Pseudomanas aeruginosa, Yersinia pseudotuberculosis, Proteus vulgaris, Enterococcus faecalis, Enterobacter cloaceae, Escherichia coli, and Listeria monocytogenes. Antibacterial activity studies were performed by agar well diffusion method. As a result of antibacterial activity studies, inhibition zones with a diameter of 14.0 ± 1.0 mm for S. epidermidis, 15.0 ± 0.5 mm for E. faecalis, 15.0 ± 0.5 mm for E. cloaceae and 16.0 ± 0.5 mm for L. monocytogenes were measured at 500 µg\mL dosage of resveratrol. However, resveratrol was found to have a bacteriostatic effect against S. typhimurium, P. aeruginosa, Y. pseudotuberculosis, P. vulgaris and E. coli. According to these results, it can be said that resveratrol may be effective against some pathogenic bacteria and may contribute to the development of new strategies for its use as an antibiotic adjuvant, although its use in terms of both food safety and public health is foreseen.Öğe DETERMINATION OF THE PRESENCE AND ANTIBIOTIC SUSCEPTIBILITY OF FRANCISELLA TULARENSIS IN SPRING WATERS IN DUZCE, TURKEY(Parlar Scientific Publications (P S P), 2017) Dülger, Görkem; Uğraş, Serpil; Dülger, BaşaranThis study aimed to investigate the presence of Francisella tularensis in the spring waters of Duzce. Water samples were collected at regular monthly intervals from 20 different stations between November 2015 and November 2016. Membrane filtration method was used for water sample filtration. In a culture study, glucose cysteine blood agar selective medium was used for F. tularensis isolation. The Tu14 primer, specific to F. tularensis, was used in PCR for molecular identification. F. tularensis was isolated from 2 of the 240 water samples. Antibiotic susceptibility of F. tularensis was determined by disk diffusion method using doxycycline, erythromycin, streptomycin, ciprofloxacin, chloramphenicol, gentamicin, meropenem, tobramycin, tetracycline, nitrofurantoin and imipenem antibiotics.The highest efficacy was demonstrated by Ciprofloxacin.Öğe Determination of Therapeutic Values of Düzce/Yığılca Honeys by Underlining Overlooked Parameters(2022) Çalışkan, Emel; Uğraş, Serpil; Kekeçoğlu, Meral; Çaprazlı, Tuğçen this study, we aimed to determine the therapeutic value of local Yığılca honeys by examining their physicochemical characteristics, phenolic content, radical scavenging activity, and antimicrobial properties by underlining some overlooked parameters. Honey samples ware divided into two main groups as CH and FH based on melissopalynologycal analysis. Antimicrobial activity of collected honey samples were investigated on important hospital-acquired infections bacteria strains; MRSA (Methicillin-resistant Staphylococcus aureus), MSSA (Methicillin-sensitive Staphylococcus aureus), Escherichia coli, and Acinetobacter baumannii. Although CH’s antimicrobial activities were found slightly higher than the FH’s, there isn’t statistically significant differences between two type honeys. However, the antioxidant activity of CH was found significantly higher than FH. Surprisingly, a higher amount of isomaltose was determined in addition to the total phenolic content in CH compared to FH. There has been found positive correlation between isomaltose amount and zone diameters for MRSA and A. baumannii. We would like to draw attention to isomaltose for its health benefit structure since these parameters may be influence honey’s therapeutic value. We recommend that isomaltose and invertase enzyme should be included in to the honey codex standards suitably depending on the monofloral and multifloral honey’s specific structure, to sensitively standardize and control their quality and therapeutic value. Our data revealed a positive correlation between antioxidant and antimicrobial activity and total phenolic content and higher isomaltose amountÖğe Etkinlik Temelli Bir Öğretim Modeli: Mikrodünyaya Yolculuk-3 Projesi(2021) Hasırcı, H. Merve Eriş; Dursun, Hacer; Uğraş, Serpil; Sipahi, Nisa; Keçeli, Fatma; Fidan, AyşenurBu çalışmada, katılımcı öğrencilerin, mikroorganizmalar konusunda farkındalık kazanması amaçlanmıştır. Bu amaç doğrultusunda, mikroorganizmaların tanımı, mikroorganizmaların fayda ve zararları, zararları durumunda alınması gereken tedbirler ve faydaları durumunda mikrobiyal biyoteknolojik üretim teknikleri konularında teorik ve uygulamalı etkinlik temelli bir öğreti modeli tasarlanmıştır. Çalışma süresince uygulanan bu etkinliklerin, katılımcıların bilimsel düşünme becerilerinin gelişmesine olan etkisi araştırılmıştır. Çalışmada katılımcı olarak hem kentsel hem de kırsal kesimden ilköğretim dönemi dördüncü sınıf gönüllü öğrenciler yer almıştır. Çalışma, iki hafta süresince, 13 erkek 17 kız olmak üzere toplam 30 katılımcı ile yürütülmüştür. Katılımcılara etkinliklerin öncesinde ve sonrasında mikroorganizmalarla ilgili mevcut bilgilerini ölçmeye yönelik 38 sorudan oluşan çoktan seçmeli başarı testi uygulanmıştır. Yapılan ölçme ve değerlendirme analiz sonuçlarına göre katılımcıların mikroorganizmalar hakkındaki bilgilerinin istatiksel olarak anlamlı bir şekilde farklılaştığı görülmüştür. Bu çalışma kapsamında öğrencilerin mikroorganizmalar ile ilgili hazır bulunuşluk düzeyi farklılıklarının, katılımcı okulların yerleşim yerlerinin farklı olmasından kaynaklı olduğu görülmüştür. Yaşanan COVID-19 Pandemi süreci, toplumun mikroorganizmalar konusunda bilinç sahibi olmadığını ve mikroorganizmaların hayatımızda ne denli ciddi sonuçlar doğurabileceğini ortaya çıkarmıştır. Bu bağlamda, pandemi sürecinde yürütülen bu çalışma, toplumdaki bireylerin mikroorganizmalar hakkında doğru bilgiler edinmesine öncülük etmekte ve bu bireylerde farkındalık oluşturmaktadır. Toplum sağlığını tehdit eden durumlarda, yüksek bilinçle hassasiyet gösteren, sürece olumlu katkılar sağlayan bireyleri topluma kazandırması açısından, böylesi çalışmalar büyük önem taşımaktadır.Öğe Evaluation of biological activity of Diplotaenia cachrydifolia Boiss. that medicinal plant(2019) Uğraş, Serpil; Ülger, Sultan; Rasgele, Pınar GöçThe aim of this study is to evaluate the antimicrobial, antioxidant, and antigenotoxic activity of extracts obtained from Diplotaenia cachrydifolia Boiss, which is traditionally used for food and medical treatment by the local people of Hakkâri Province(Turkey). As a result of this study, the extracts of D. cachrydifolia exhibited a broad antimicrobial spectrum and high antimicrobial activity. It was also observed that the extracts had a high antioxidant activity. The DPPH $\;IC_{50}$ values of the extracts were2.5234 µg/mL, 6.0075 µg/mL, and 29.1974 µg/mL for ethanol, acetone, and hexane, respectively. The highest phenol contentwas found to be 55.36±0.035 µg/mL for ethanol. Furthermore, the ethanol extract was found to have a protective effect againstmitomycin C-induced genotoxicity. D. cachrydifolia has a high biological activity; therefore, it is anticipated that future pharmacological studies will shed more light on this plant.Öğe Herniaria glabra L. Bitkisinin Biyolojik Aktivitesinin Belirlenmesi(Osman SAĞDIÇ, 2021) Üzmez, Şebnem; Uğraş, SerpilBu çalışmada, Herniaria glabra L. bitkisinin antibakteriyal, antioksidan ve fenolik bileşik tayinini kapsayan biyolojik karakteristiklerinin aydınlatılması hedeflenmiştir. Bu amaç doğrultusunda ilk olarak sırasıyla, aseton, etanol ve hekzan çözücüleri kullanılarak bitki ekstraktları elde edilmiştir. Elde edilen bitki ekstraklarının Enterobacter cloaceae ATCC 13047, Enterococcus faecalis ATCC 29212, Salmonella typhimirium ATCC 14028, Staphylococcus epidermidis ATCC 12228, Proteus vulgaris ATCC 29905, Yersinia pseudotuberculosis ATCC 911, Staphylococcus aureus ATCC 25923, Pseudomanas aeruginosa ATCC 27853, Klebsiella pneumoniae ATCC 13883, Bacillus subtilis ATCC 6633, Escherichia coli ATCC 35218 ve Listeria monocytogenes ATCC 7644 bakterilerine karşı agar kuyu difüzyon metodu ile antibakteriyal aktiviteleri test edilmiştir. Antioksidan aktivite analizi DPPH (2,2-Difenil-1-pikrilhidrazil) serbest radikal giderme yöntemi ile tanımlanmış ve toplam fenolik bileşen analizi Folin-Ciocalteu metoduna göre gerçekleştirilmiştir. Bu çalışmaların sonucunda, en yüksek verimle (% 2.4476) elde edilen aseton ekstraktının en yüksek fenolik içeriğe (6.25 ± 0,0012 µg/ml) sahip olduğu belirlenmiştir. Yine aseton ekstraktının DPPH radikal süpürücü aktivitesinin (IC50; 20.5610 µg/ml) etanol ve hekzan ekstratlarından yüksek olduğu gözlenmiştir. Etanol, hekzan ve aseton ekstraklarının K. pneumoniae bakterisine karşı yüksek inhibisyon aktivitesine sahip olduğu ancak diğer indikatör bakterilere karşı inhibisyon aktivitesinin olmadığı belirlenmiştir.Öğe İlköğretim Öğrencilerinde Proje Tabanlı Mikroskobik Canlı Bilincinin Oluşturulması(2017) Uğraş, Serpil; Keskin, Hasan Kağan; Sipahi, Nisa; Dursun, HacerBu çalışmada, günlük yaşamın bir parçası olan mikroorganizmalar hakkında genelbilgilerin verilmesi ve özellikle zararlı mikroorganizmalara karşı doğru antibiyotikkullanımının öğretilmesi hedeflenmiştir. Çalışma, hem kırsal hem de kentselkesimden 5. Sınıfta öğrenim gören, 20’si erkek, 18’i kız olmak üzere toplam 38öğrenci ile yürütülmüştür. Katılımcılara, uygulamalar öncesinde ve sonrasındamikroorganizmalarla ilgili mevcut bilgilerini belirlemek amacıyla, 38 sorudan oluşanbaşarı testleri uygulanmıştır. Başarı testi sonucunda elde edilen veriler normaldağılıma sahip olmadığından, farklı gruplara ait verilere Mann Whitney U testi, aynıgruba ait verilere ise Wilcoxon İşaretli Sıralar Testi uygulanmıştır. Analiz sonuçlarınagöre katılımcıların mikroorganizmalar hakkındaki öğrenme çıktıları istatistikselolarak anlamlı bir şekilde farklılaşmıştır. Araştırmada yerleşim yeri değişkenine aitbetimleyici istatistikler sonucunda ise okulun bulunduğu yerleşim yerinin kırsal veyakentsel olmasının öğrencinin mikroorganizmalar konusuna ait hazırbulunuşluk düzeyive öğrenme çıktılarının üzerinde belirleyici bir etkisinin olduğu görülmüştür. Buçalışma sayesinde yapılan etkinliklerle kavramlar öğretilirken aynı zamandakatılımcıların bilime bakış açılarında önemli ölçüde ilerlemeler kaydedilmiştir. Buçalışma konu itibarı ile geleceğimiz ve sağlığımız için son derece önemli bir eğitiminparçası olma niteliğindedir.Öğe Isolation, identification and characterization of probiotic properties of bacterium from the honey stomachs of Yigilca honeybees in Turkey(Entomological Soc Turkey, Ege Univ, 2017) Uğraş, SerpilHoneybees are considered as a key species in nature for their vital role in the maintenance of almost all life on earth. However, the massive death of honeybee stocks worldwide, largely due to colony collapse disorder, is causing international concern. In order to avoid these losses, new approaches must be sought. In previous studies, the probiotic properties of the bacteria found in the bodies of honeybees are thought to have an active role in providing resistance against pathogens. Consequently, in this study, it is aimed to isolate probiotic lactic acid bacteria from honey stomachs of the healthy honeybee, to examine the effect of these bacteria against pathogenic bacteria and to use these bacteria to boost the immune system of bees. For this purpose, between 2015 and 2016, probiotic bacteria were screened from honey bees that provided by DAGEM (Duzce University, Beekeeping Research, Development and Application Center, Yigilca, DUZCE). The inhibitory activity of the obtained bacteria against the bee pathogen Melissococcus plutonius (Truper and de 'Clari, 1998) (Enterococcaceae) was determined by in vitro agar well diffusion. The bacterium with the desired characteristics were identified by biochemical, physiological and 16s rDNA analysis as Lactobacillus kunkeei (Edwards, 1998) (Lactobacillaceae) and its probiotic nature was investigated. With the evaluation of these findings, future preparations of the isolate are expected to support the bee immune system and, as a result, to produce resistant honeybees without resorting to treatment with antibiotics.Öğe Isolation, Identification and Enzyme Characterization of Lipase Producing Bacteria from Mucus Layer of Oncorhynchus mykiss(Hacettepe Üniversitesi, 2016) Uğraş, SerpilIn this study, 13 different bacteria were isolated from the surface mucus layer of Oncorhynchus mykiss ra- inbow trout . These bacteria were identified as Exiguobacterium sp. Om1 , Acinetobacter sp. Om2, Om7 , Microbacterium sp. Om3 , Arthrobacter sp. Om4 , Sphingobacterium sp. Om5 , Stenotrophomonas sp. Om6, 10 and 11 , Pseudomanas sp. Om8 , Corynebacterium sp. Om12 Aeromonas sp. Om14 and Psychrobacter sp. Om15 based on morphological, physiological, and biochemical characteristics, as well as phylogenetic analy- sis using 16S rDNA sequences. We report that isolate Om15 produces a cold-active lipase enzyme. The lipase enzyme was partially purified from the bacterial supernatant and its specific activity was calculated as 64.393 U g–1. Optimal performance of the enzyme occurred at pH 8.0 and 20°C using p-nitrophenyl dodecanoate as a substrate. SDS-PAGE indicated that the lipase enzyme is composed of 2 subunits 58-60 kDa, and that it is possible that the active lipase enzyme is the heterodimer of the subunits, which was confirmed via Native- PAGE. Furthermore, the lipase activity decreased in response to application of Co2+ and Cu2+ ions; however, no significant difference in the lipase activity was observed via application of other ions.Öğe POTENTIAL USE OF FIRSTLY ISOLATED BACTERIA FROM YIGILCA HONEYBEES (APIS MELLIFERA L.) AND PRODUCTS AGAINST THE PATHOGENS(Parlar Scientific Publications (P S P), 2017) Uğraş, Serpil; Dursun, Hacer; Dülger, Görkem; Kekeçoğlu, MeralThe honeybees are vital to the continuity of almost all living things on earth. Unfortunately, honeybees are under the threat of many pathogens as Paenibacillus larvae. These pathogens cause severe loss in the hives resulting in bee colony extinction, which is a cause for international concern because it results in massive deaths of commercial honeybee stocks. Due to the economic and ecological importance of honeybees, it is essential to develop effective, sustainable and environmentally friendly strategies for the control of bee diseases. Among these strategies, the use of environmentally friendly biological control preparations which will be developed from its own microbiota is very important. With the idea that honeybees may be more resistant to diseases if the microbiota are supported, this study aimed to first determine the bacteria found in healthy honeybees and their products. In line with this goal, nineteen bacteria were identified with molecular techniques. These included: in the honey, Staphylococcus warneri (HD5, HD20); in the pollen, Lactobacillus kunkeei (HD6), Fructobacillus fructosus (HD8), S. lentus (HD9), Pantoea vagans (HD10), Bacillus licheniformis (HS6), Pluralibacter pyrinus (HS10) and P. anthophila (HS11); in bee bread, L. kunkeei (HD12), P. agglomerans (HD11), B. cereus (HS1 and HS3) and B. safensis (HS2 and HS4); and in the bees, B. safensis (HD18), Escherichia coli (HD13) and Enterobacter cloacae (HS19, HS20). Furthermore, it was determined that L. kunkeii (HD6 and HD12) and F. fructosus (HD8) had high inhibition activity against the American foulbrood agent P. larvae.Öğe Synthesis of New 3,5-disubstituted-1,2,4-Triazoles and Evaluation of Antibacterial, Antiurease and Antioxidant Activities(Chem Soc Pakistan, 2016) Gümrükçüoğlu, Nurhan; Sökmen, Bahar Bilgin; Şahin, Hüseyin; Uğraş, Serpil; Sağkal, Yasemin; Uğraş, Halil İbrahimAcylhydrazone 2 was synthesized by the condensation of iminoester hydrochloride 1 with acyl hydrazine. The treatment of acylhydrazone with hydrazine hydrate afforded 4-amino-3,5-dialkyl-1,2,4-triazole 3. The treatment of compound 3 with various aromatic aldehydes resulted in the formation of 4-arylidenamino-3, 5-dialkyl-1,2,4-triazoles 4a-c. Sodium borohydride reduction of 4-arylidenamino derivatives afforded 4-alkylamino-3,5-dialkyl- 1,2,4-triazoles 5a-c. The obtained products were identified by FTIR, H-1-NMR, C-13-NMR, Mass spectroscopic and elemental analysis. A series of compounds were evaluated for their, antibacterial, antiurease, antioxidant activities. The results showed that the synthesized new compounds had effective antibacterial, antioxidant, antiurease activities.Öğe Synthesis, Antibacterial, Antiurease, and Antioxidant Activities of Some New 1,2,4-Triazole Schiff Base and Amine Derivatives(Humana Press Inc, 2015) Sökmen, Bahar Bilgin; Gümrükçüoğlu, Nurhan; Uğraş, Serpil; Şahin, Hüseyin; Sağkal, Yasemin; Uğraş, Halil İbrahimThe acylhydrazone compound named ethyl N'-furan-2-carbonylbenzohydrazonate was synthesized by the condensation of ethyl benzimidate hydrochloride with furan-2-carbohydrazide. The treatment of the acylhydrazone with hydrazine hydrate afforded 4-amino-3-furan-2-yl-5-phenyl-1,2,4-triazole. The usage of this compound with various aromatic aldehydes resulted in the formation of 4-arylidenamino-3-furan-2-yl-5-phenyl-1,2,4-triazoles. Sodium borohydride reduction of 4-arylidenamino derivatives afforded 4-alkylamino-3-furan-2-yl-5-phenyl-1,2,4-triazoles. The obtained products were identified by FT-IR, H-1-NMR, C-13-NMR. A series of compounds were evaluated for their antibacterial, antiurease, and antioxidant activities. The results showed that the synthesized new compounds had effective antiurease and antioxidant activities.
