Herniaria glabra L. Bitkisinin Biyolojik Aktivitesinin Belirlenmesi
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Date
2021
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Osman SAĞDIÇ
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info:eu-repo/semantics/openAccess
Abstract
Bu çalışmada, Herniaria glabra L. bitkisinin antibakteriyal, antioksidan ve fenolik bileşik tayinini kapsayan biyolojik karakteristiklerinin aydınlatılması hedeflenmiştir. Bu amaç doğrultusunda ilk olarak sırasıyla, aseton, etanol ve hekzan çözücüleri kullanılarak bitki ekstraktları elde edilmiştir. Elde edilen bitki ekstraklarının Enterobacter cloaceae ATCC 13047, Enterococcus faecalis ATCC 29212, Salmonella typhimirium ATCC 14028, Staphylococcus epidermidis ATCC 12228, Proteus vulgaris ATCC 29905, Yersinia pseudotuberculosis ATCC 911, Staphylococcus aureus ATCC 25923, Pseudomanas aeruginosa ATCC 27853, Klebsiella pneumoniae ATCC 13883, Bacillus subtilis ATCC 6633, Escherichia coli ATCC 35218 ve Listeria monocytogenes ATCC 7644 bakterilerine karşı agar kuyu difüzyon metodu ile antibakteriyal aktiviteleri test edilmiştir. Antioksidan aktivite analizi DPPH (2,2-Difenil-1-pikrilhidrazil) serbest radikal giderme yöntemi ile tanımlanmış ve toplam fenolik bileşen analizi Folin-Ciocalteu metoduna göre gerçekleştirilmiştir. Bu çalışmaların sonucunda, en yüksek verimle (% 2.4476) elde edilen aseton ekstraktının en yüksek fenolik içeriğe (6.25 ± 0,0012 µg/ml) sahip olduğu belirlenmiştir. Yine aseton ekstraktının DPPH radikal süpürücü aktivitesinin (IC50; 20.5610 µg/ml) etanol ve hekzan ekstratlarından yüksek olduğu gözlenmiştir. Etanol, hekzan ve aseton ekstraklarının K. pneumoniae bakterisine karşı yüksek inhibisyon aktivitesine sahip olduğu ancak diğer indikatör bakterilere karşı inhibisyon aktivitesinin olmadığı belirlenmiştir.
In this study, it was aimed to elucidate the biological characteristics of Herniaria glabra L. plant, including determination of antibacterial, antioxidant and phenolic compounds. For this purpose, firstly, plant extracts were obtained by using acetone, ethanol and hexane solvents, respectively. The obtained extracts were tested for antibacterial activity with agar well diffusion assay against Enterobacter cloaceae ATCC 13047, Enterococcus faecalis ATCC 29212, Salmonella typhimirium ATCC 14028, Staphylococcus epidermidis ATCC 12228, Proteus vulgaris ATCC 29905, Yersinia pseudotuberculosis ATCC 911, Staphylococcus aureus ATCC 25923, Pseudomanas aeruginosa ATCC 27853, Klebsiella pneumoniae ATCC 13883, Bacillus subtilis ATCC 6633, Escherichia coli ATCC 35218 and Listeria monocytogenes ATCC 7644 strains. The antioxidant activities of extracts were determined by DPPH (2,2-Diphenyl-1-picrilhydrazil) free radical removal method and total phenolic component analysis was conducted according to Folin-Ciocalteu method. As a result of these studies, it was determined that the acetone extract obtained with the highest yield (2.4476%) had the highest phenolic content (6.25 ± 0.0012 µg/ml). It was also observed that DPPH radical scavenging activity (IC50; 20.5610 µg/ml) of acetone extract was higher than that of ethanol and hexane extracts. It was determined that ethanol, hexane and acetone extracts had high inhibitory activity against K. pneumoniae bacteria, but no inhibition activity against other indicator bacteria.
In this study, it was aimed to elucidate the biological characteristics of Herniaria glabra L. plant, including determination of antibacterial, antioxidant and phenolic compounds. For this purpose, firstly, plant extracts were obtained by using acetone, ethanol and hexane solvents, respectively. The obtained extracts were tested for antibacterial activity with agar well diffusion assay against Enterobacter cloaceae ATCC 13047, Enterococcus faecalis ATCC 29212, Salmonella typhimirium ATCC 14028, Staphylococcus epidermidis ATCC 12228, Proteus vulgaris ATCC 29905, Yersinia pseudotuberculosis ATCC 911, Staphylococcus aureus ATCC 25923, Pseudomanas aeruginosa ATCC 27853, Klebsiella pneumoniae ATCC 13883, Bacillus subtilis ATCC 6633, Escherichia coli ATCC 35218 and Listeria monocytogenes ATCC 7644 strains. The antioxidant activities of extracts were determined by DPPH (2,2-Diphenyl-1-picrilhydrazil) free radical removal method and total phenolic component analysis was conducted according to Folin-Ciocalteu method. As a result of these studies, it was determined that the acetone extract obtained with the highest yield (2.4476%) had the highest phenolic content (6.25 ± 0.0012 µg/ml). It was also observed that DPPH radical scavenging activity (IC50; 20.5610 µg/ml) of acetone extract was higher than that of ethanol and hexane extracts. It was determined that ethanol, hexane and acetone extracts had high inhibitory activity against K. pneumoniae bacteria, but no inhibition activity against other indicator bacteria.
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Keywords
Antibakteriyal aktivite|Antioksidan aktivite|Ekstraksiyon|Fenolik bileşen|Herniaria glabra.|Antibacterial activity|Antioxidant activity|Extraction|Herniaria glabra|Phenolic component.
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Avrupa Bilim ve Teknoloji Dergisi
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27
