Yazar "Dülger, Görkem" seçeneğine göre listele
Listeleniyor 1 - 20 / 35
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe Anti-Staphylococcal Activity of Verbascum thapsus L. against Methicillin-Resistant Staphylococcus aureus(Duzce Univ, 2017) Dülger, Görkem; Tütenocaklı, Tülay; Dülger, BaşaranObjective: In this study, it was aimed to investigate the anti-Staphylococcal activity of ethanol extract obtained from leaves of Verbascum thapsus L. (Scrophulariaceae) plant. Methods: The anti-Staphylococcal activity of the extract of ethanol extracted from the leaves of Verbascum thapsus L. (Scrophulariaceae) was investigated against Methicillin-Resistant Staphylococcus aureus (MRSA) bacteria by Agarwell diffusion method and microdilution method. Results: The ethanol extract showed antibacterial activity against Methicillin-resistant Staphylococcus aureus (MRSA) strains (MIC < 1024 mu g/mL). When compared with Methicillin and Gentamicin, the extract was more effective against MRSA strains. Conclusion: The use of Verbascum thapsus L. (Scrophulariaceae) as an anti-Staphylococcal agent has been found to be appropriate in vitro and should be performed in vivo.Öğe Antibacterial activity of Codium fragile on common fish pathogens(Journal of Pure and Applied Microbiology, 2014) Dülger, Görkem; Dülger, BaşaranMethanol, dichloromethane and hexane extracts of Codium fragile (Suringar) Hariot (Chlorophyta) were tested for antibacterial activity against common fish pathogens (Aeromonas hydrophila, Yersinia ruckeri, Streptococcus agalactia and Enterococcus faecalis) by microdilution method. The hexane extract of the alga has shown a strong antibacterial activity as MIC and MBC against Aeromonas hydrophila at 64 (>128) ?g/mL concentrations and Yersinia ruckeri at 128 (128) ?g/mL concentrations, respectively. While methanol extracts showed weak antibacterial activity against all bacterial pathogens, the dichloromethane extracts showed activity against the test microorganisms. The results demonstrate that the hexane extract of the alga has significant antibacterial activity and suggest that it may be useful in the treatment of bacterial fish diseases.Öğe Antibacterial Activity of Stachys sylvatica Against Some Human Eye Pathogens(Natural and Engineering Science, 2022) Dülger, Görkem; Dülger, BaşaranThe ethanol extracts obtained from the leaves of Stachys sylvatica L. (Lamiaceae) were evaluated for their antibacterial activity against some human eye pathogens by disc diffusion method against hospital isolates of Bacillus cereus, Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus capitis, Staphylococcus aureus, Staphylococcus epidermidis, Moraxella nonliquefaciens and Cutibacterium acnes. Besides, Penicillin, Ampicillin and Tobramycin as antibacterial antibiotics were used as a positive reference standard to determine the sensitivity of the isolates. The extracts showed a potential antibacterial effect against Staphylococcus capitis, Moraxella nonliquefaciens and Cutibacterium acnes, with inhibition zones of 22.4 mm, 21.6 mm and 20.2 mm, respectively. The extracts also exhibited a moderate effect against the other bacterial isolates. The plant may be useful in the treatment of eye infections. © 2022, Natural and Engineering Science. All rights reserved.Öğe Antibacterial Activity of Verbascum antinori(Duzce Univ, 2018) Dülger, Başaran; Dülger, GörkemObjective: In current study, biological screening of the methanol extract obtained from Verbascum antinori Boiss. et Heldr. (Scrophulariaceae) was carry out on antibacterial effects. Methods: Antibacterial activity of the extract was evaluated for Staphylococcus aureus ATCC 6538P, Proteus vulgaris ATCC 8427, Klebsiella pneumoniae UC57, Micrococcus luteus CCM 169, Escherichia coli ATCC 11230, Listeria monocytogenes ATCC 15313, Bacillus cereus ATCC 7064 and Pseudomonas aeruginosa ATCC 27853 by disk diffusion method. Results: The extract has potential effect against the Gram-positive bacteria, but no activity was observed against the Gram-negative bacteria used in the present study. Conclusions: On the basis of the mentioned results, V. antinori may be a potential source of antibacterial agent.Öğe Antifungal Activity of Hypericum havvae Against Some Medical Candida Yeast and Cryptococcus Species(Pharmacotherapy Group, 2014) Dülger, Görkem; Dülger, BaşaranPurpose: To investigate the antifungal activities. of the individual as well as the combined hydroalcohol leaf and root extracts of endemic Hypericum havvae A. Guner (Hyperiaceae). Methods: Each dry powdered plant material (20 g) was soaked in 150 ml of aqueous ethanol (50: 50 % v/v) until complete saturation of the plant material. After the extracts were filtered and evaporated, the antifungal activity of the extracts was tested against medical yeast, Candida (C. albicans ATCC 10231, C. tropicalis ATCC 13808, C. guilliermondii ATCC 6260) and Cryptococcus (C. neoformans ATCC 90112 and C. laurentii 34142) species by visual broth microdilution method. Minimum inhibitory concentration (MIC) of the extract was derived from the results. Ketoconazole was used as the reference standard. Results: MIC values ranged from 3.12 to 25.00 mg/ml. The extracts exhibited strong antifungal effect against the yeast cultures but the combination of the plant extracts (leaf + root) possessed stronger antifungal potency against Candida albicans and Cryptococcus laurentii, with the same MIC value of 1.56 mg/ml. Conclusion: Our findings support the use of Hypericum havvae in traditional medicine for the treatment of fungal infections, especially Candidiasis.Öğe Antifungal activity of Salvia verticillata subsp. verticillata against fungal pathogens(2021) Dülger, Görkem; Dülger, BaşaranAim: It was aimed to investigate the antifungal activity of the individual as well as the combine extract (leaf+root) ofSalvia verticillata subsp. verticillata L. from Lamiaceae family which has ethnobotanical use.Material and Methods: The plant samples were collected during to flowering stage in June, Samandere village around,Duzce, Turkey. The collected plants were dried according to herbarium techniques. The powdered plant material wasextraction using ethyl alcohol (96%). Antifungal effects of the extracts was evaluated for yeasts (Candida krusei ATCC20298, C. parapsilosis ATCC 90112, C. guilliermondii ATCC 6260, C. albicans ATCC 10231, C. tropicalis ATCC13808, C. glabrata ATCC 2001, Cryptococcus laurentii ATCC 34142, C. neoformans ATCC 90112, Geotrichumcapitatum ATCC 28576, Debaryomyces hansenii DSM 70238, Rhodotorula rubra DSM 70403, and Kluyveromycesfragilis ATCC 8608) by visual broth microdilution method. Antifungal antibiotic ketoconazole was used in the study tocompare the results.Results: MIC values ranged from 1.56 to 25.00 mg/mL. The extracts have shown potential antifungal effects againstthe yeast cultures. Notably, the combine plant extracts (both leaves and rootstock) possessed higher antifungal potencyagainst C. neoformans, C. laurentii and G. capitatum.Conclusion: Our results demonstrated that the extracts of Salvia verticillata subsp. verticillata are used for thetreatment of fungal infections as traditional medicine.Öğe Antifungal Activity of Soil Streptomyces Isolates Against Cryptococcus neoformans(2021) Dülger, Başaran; Dülger, GörkemIn this study, 26.5% of 128 different isolates of Streptomyces recovered from soils in Duzce province, Turkey showed antifungal activity against Cryptococcus neoformans ATCC 90112. Considering the diameter of the inhibition zone formed on the agar plate, isolates were divided into four sections: section 1 (5-10 mm, slightlyactive); section 2 (11-15 mm, moderately-active), section 3 (16-25 mm, highly-active) and section 4 (26-35 mm, ultra-active). It is determined that 3 isolates in section 4 may be a source of novel antibiotic against Cryptococcosis.Öğe Antimicrobial and Anticancer Activity of Corydalis solida(2022) Dülger, GörkemAim: The present study, it was aimed to evaluate the bioactive properties of Corydalis solida. Material and Methods: In the study, the anticancer activity of ethanolic extracts prepared from C. solida was determined on HCT116 colon cancer, AGS gastric cancer and HepG2 hepatocellular carcinoma cell lines and HUVEC cells, healthy control cell line. Well diffusion method was used to determine the antimicrobial properties of solida. For this purpose, ethanolic extracts were used for antimicrobial activity against four bacterial isolates (Escherichia coli, Bacillus cereus, Staphylococcus aureus and Klebsiella oxytoca) and three yeast strains (Candida albicans, C. tropicalis and C .glabrata). Results: Corydalis solida plant extract produced significant antiproliferative effect in HCT116 (colon cancer), AGS (gastric cancer) and HepG2 (liver cancer) cell lines. This effect was more remarkable in the HepG2 cell line. In addition, negligible cell death in HUVEC cells indicated that the plant was not toxic to healthy cells. Plant extract application also caused significant Caspase-3, 8 and 9 activation in HepG2 and HCT116 cells, consistent with the antiproliferative effect. Antimicrobial studies have shown that the extract made inhibition zone on bacteria. Conclusion: In the study, it was determined that the ethanol extract of Corydalis solida had anticancer effect. In addition, the extract had inhibitory properties on bacteria. The data obtained from the study are qualified to support further pharmacological studies.Öğe The antimicrobial effect of various formulations obtained from Fomes fomentarius against hospital isolates(2019) Gedık, Gulsah; Dülger, Görkem; Asan, Hulya; Özyurt, Anıl; Allı, Hakan; Asan, AhmetThe purpose of this study was to investigate the antimicrobial activity of formulations of F. fomentarius. The antimicrobial efficacy levels of extracts of F. fomentarius prepared from ethanol, water and of various formulations (emulsion, ointment, paste, cream and gel) were determined. For this purpose, the disk diffusion method was used to test the extracts for antimicrobial activity against hospital isolates including Klebsiella pneumoniae, Acinetobacter baumannii, Staphylococcus aureus, vancomycin-resistant enterococci (VRE+), Escherichia coli, Candida krusei, C. albicans, C. tropicalis, C. guilliermondii and C. glabrata. Some standard antibiotics were used for comparison. The ointment formulation (No. 4) showed a good antimicrobial effect on bacteria compared to the antibiotics (9.6 - 16.1 mm.). The extracts were found to be more effective than the antibiotics against Candida species. Formulation No. 4 was considered to be more effective because of the protective nature of its cetyl alcohol and sodium lauryl sulfate (SLS) content . F. fomentarius ethanol extract, F. fomentarius water extract and gel formulation showed similar activity; however, other formulations (emulsion, paste, cream) were generally more effective due to the stabilizing effects of the polymers, oil and alcohols present in their preparations. The findings of this study establish the possibility of discovering new clinically effective antibiotic drugs and could be useful in understanding the relationship between traditional remedies and modern medicines.Öğe Antimicrobial, Antiproliferative Effects and Docking Studies of Methoxy Group Enriched Coumarin-Chalcone Hybrids(John Wiley and Sons Inc, 2023) Badreddin Musatat, Ahmad; Kılıçcıoğlu, İlker; Kurman, Yener; Dülger, Görkem; Alpay, Merve; Yağcı, Ravza; Atahan, AlparslanMethoxy group enriched eight coumarin-chalcone hybrid derivatives were synthesized. Antimicrobial/ antiproliferative activities were tested against eight human pathogenic microorganisms and four cancer cell lines (AGS, HepG2, MCF-7 and PC-3), respectively. Antimicrobial results showed that most of the compounds were almost more active than used standard antibiotics. Cytotoxicity results showed that 2,3,4-trimethoxyphenyl and thiophene containing structures have promising antiproliferative effects against AGS gastric cell lines with ?5 ?g/ml IC50 values. At the same time, 2,4-dimethoxyphenyl bearing derivative exhibited the lowest IC50 values against HepG2 (?10 ?g/ml) and PC-3 (?5 ?g/ml) cell lines. Particularly, the cell viabilities of MCF-7 cell lines were remarkably inhibited by all the compounds with lower IC50 values. Therefore, molecular docking studies between hybrid ligands and quinone reductase-2 enzyme (regulates in MCF-7 cancer cells) were performed. The results demonstrated that all the derivatives can smoothly interact with interested enzyme in agreement with the experimental results. Finally, ADME parameters were studied to reveal drug-likeness potentials of the coumarin-chalcone hybrids. © 2023 Wiley-VHCA AG, Zurich, Switzerland.Öğe Biological evaluation of benzothiazoles obtained by microwave-green synthesis(Acad Brasileira De Ciencias, 2024) Ozdincer, Mesut; Dalmaz, Aslihan; Durmus, Sefa; Dülger, Görkem; Kiliccioglu, IlkerBenzothiazole compounds are known as an important bicyclic ring system with multiple applications. These compounds have a wide range of biological activities, including anticancer, antimicrobial, anti-inflammatory and antiviral activities. In this study, benzothiazole compounds were synthesized and their various biological activities were examined. The synthesized benzothiazoles were evaluated for their antimicrobial properties against various bacterial and fungal strains. The compound 6e is most active ligand in the series against bacteria and fungi as compared to standard antibiotics. Especially, this compound significant effect against Staphylococcus aureus (32.00 +/- 1.73 mm). These compounds exhibited potent anticancer activity against gastrointestinal cancer cells, demonstrating their potential as therapeutic agents. The lowest antiproliferative response after administration of the compounds was observed in HCT116 cells, while the most effective antiproliferative response was observed in AGS cells (> 10 mu g/mL). In all cell lines, 40 and 100 mu g/mL application values of the selected compounds showed significant increases in the expression of caspase-3, 8 and 9. We also utilized a computational docking approach to investigate the interaction of these benzothiazoles with VEGFR-2 kinase. Our docking studies showed that compounds 6a and 6d may be promising therapeutic agents against gastrointestinal system cancers due to their ability to bind to VEGFR-2 kinase.Öğe Castor oil-based graft copolymers: synthesis, characterization antimicrobial activity and antiproliferative effects against breast cancer cell lines(Springer, 2022) Allı, Sema; Dülger, Görkem; Kılıççıoğlu, İlker; Allı, Abdulkadir; Dülger, BaşaranCastor oil was autoxidized for three months to obtain macroperoxide castor oil (PCO). Since this polymeric oil has peroxide groups and hydroxyl groups, free-radical polymerization (FRP), ring-opening polymerization, and one-pot polymerization both free-radical polymerization (FRP) and ring-opening polymerization in one step methods were used to synthesis of various types of the graft copolymers. PCO-g-Polystyrene, PCO-g-Poly(methyl acrylate), and PCO-g-Poly(tert-buytl acrylate) were synthesized using the free-radical polymerization method. PCO-g-Poly(epsilon-caprolactone) graft copolymer was obtained via ring-opening polymerization, and PCO-g-Polystyrene-g-Poly(epsilon-caprolactone) graft copolymer was obtained using one-pot polymerization method. All graft copolymers obtained were characterized by proton nuclear magnetic resonance (H-1 NMR), Fourier-transform infrared, differential scanning calorimetry, thermal gravimetric analysis, and elemental analysis. After the synthesis of castor oil-based copolymers, we determined the antimicrobial effects of them as well as the antiproliferative effects on breast cancer cell lines. Antimicrobial activity was carried out using the disc diffusion method and the dilution method. As a result of antimicrobial activity, the compounds showed moderate antimicrobial effects on microorganisms compared to standard antibiotics. In particular, the compounds have shown more antibacterial activity than antifungal activity. We also observed a prominent antiproliferative effect of synthesized compounds depending on increasing doses (1-25 mu g/mL) and times (24-48 h) in MDA-MB-231 breast cancer cells.Öğe Chemical Composition and In Vitro Antimicrobial, Antioxidant, and Antiproliferative Studies of the Lamium galeobdolon L. (L.) Essential Oil(Maik Nauka/Interperiodica/Springer, 2022) Akkoyunlu, A.; Dülger, GörkemThe current study aimed to evaluate the chemical composition and bioactive properties of Lamium galeobdolon (L.) L. After obtaining an ethanol extract, analysis of its chemical composition was carried out via gas chromatography-mass spectroscopy (GC-MS), which identified 2(3H)-benzoxazolone, palmitic acid, 3,7,11,15-tetramethyl-2-hexadecen-1-ol, eicosane, 9,12-octadecadienoic acid, and tetracosane as major components. In addition, the antioxidant and oxidant capacities of the essential oil were determined using the total antioxidant status (TAS) and the total oxidant status (TOS) kits, and the oxidative stress index (OSI) value was calculated from the ratio of the two. The OSI value of L. galeobdolon (L.) L. was found to be 0.987 arbitrary units (AU). Essential oil was obtained from the plant and the antimicrobial and antiproliferative effects of this oil were investigated. The antiproliferative activity was determined via MTT assay on the B16F10 cell line. The maximum cell death was observed as 88.9% at a concentration of 50 mu g/mL. The antimicrobial activity was determined via the disc diffusion method. Accordingly, no antibacterial activity was found; however, the antifungal effect of the plant was significant.Öğe Chemical Composition of Lamium purpureum L. and Determination of Anticancer Activity of Its Essential Oil on Melanoma(2019) Akkoyunlu, Aysegul; Dülger, GörkemThe current study aimed to evaluate Lamium purpureum L. in terms of its chemical composition and thepotential of its essential oil for anticancer activity. The profiling analysis of its chemical composition was carriedout via GC-MS. For this purpose, the ethanol extract was subjected to GC-MS analysis to determine chemicalcompounds. To obtain essential oil, steam distillation of the whole plant (aerial, leaf, stem) was carried out in aClevenger apparatus. The anticancer activitiy was determined via MTT assay. We observed that the maximumcell death was 14% at 50 µg/mL concentration. As a result of GC-MS analysis, palmitic acid, 7-tetradecenal,octadecadienoic acid, acetic acid, ethyl 9,12,15-octadecatrienoate, hexatriacontane, stearate and benzyl benzoatewere identified as major components. We determined that the essential oil of L. purpureum L. had essentialcompounds which showed cytotoxic activities.Öğe Chemical composition, antioxidant, antimicrobial and anticancer activities of endemic Cephalaria tuteliana(Elsevier, 2024) Kiliccioglu, Ilker; Dülger, Görkem; Senturk, Fatih; Bozyel, Mustafa Eray; Canli, Kerem; Dülger, BaşaranIntroduction: The genus Cephalaria, belonging to the Caprifoliaceae family, is a rich source of secondary metabolites, including mainly saponins which display a variety of biological activities, such as antimicrobial, antioxidant, and anticancerous effects. This study was carried out to observe the biochemical composition and to evaluate the biological activities of Cephalaria tuteliana Kus & Gokturk. Methods: The composition of endemic C. tuteliana was determined through gas chromatography-mass spectrometry (GC-MS) studies. Antimicrobial effect were determined by the disk diffusion and dilution methods for test bacteria and test fungi. The antioxidant potential of the ethanol extract was also investigated. Cell proliferation and apoptosis analysis were investigated by WST-1 and ELISA methods. The migration level of PC-3 prostate cancer cells after plant extract application was examined by wound healing assay. Also, molecular docking experiments were performed to investigate the anticancer activity of the major component found in the C.tuteliana extract. Results: GC-MS analysis showed that the predominant compounds were Phenol, 2,20-methylenebis [6-(1,1dimethyl ethyl)-4-methyl- (23.54 %), 9,12,15-octadecatrien-1-ol, (Z,Z,Z)-(6.06 %) and n-Hexadecanoic acid (3.03 %). The ethanolic extract of C. tuteliana displayed moderate antimicrobial activity against a spectrum of tested microorganisms. Also, it exhibited significant antiproliferative effects on PC-3 human prostate cancer cells while having no harmful effects on healthy control HUVEC cells. Furthermore, enhanced expressions of Caspase-3, 8, and 9 were observed in PC-3 cells after plant extract treatment. Also, cell migration level was notably reduced, especially at a dose of 400 mg/mL in PC-3 cells. The DPPH free radical scavenging activity of ethanolic extracts of C. tuteliana showed higher activity than ascorbic acid. Molecular docking experiments showed potential binding of the major aromatic compound in C.tuteliana extract to human androgen receptor 1E3G protein. Conclusion: These findings highlight the potential of C. tuteliana as a valuable natural resource and pave the way for further in vivo prostate cancer investigations. (c) 2024 SAAB. Published by Elsevier B.V. All rights reserved.Öğe Chemical Compositions and Antimutagenic Effects of Ethanolic Extracts of Stachys Thirkei and Stachys Annua subsp. Annua Using the Ames Assay(Springer, 2021) Rasgele, Pinar Goc; Dülger, GörkemIn this study, antimutagenic effects of ethanolic extracts of Stachys thirkei and Stachys annua subsp. annua belonging to Lamiaceae family that grow in Duzce of Turkey were investigated on Salmonella typhimurium TA98 and TA100 strains without metabolic activation using Salmonella/microsome test system. Three different concentrations (0.025, 0.05 and 0.1 mg/plate) of the ethanolic extracts of S. thirkei and S. annua subsp. were used as active materials in which the major components were 2-ethylhexanol (30.012%) and 2-beta-pinene (26.40%), respectively. The highest concentration (0.1 mg/plate) of S. thirkei had a moderate antimutagenic effect on the Salmonella typhimurium TA98 strain with a 26.79% inhibition rate. It was also established that the extract of S. thirkei at highest concentration (0.1 mg/plate) exhibited strong antimutagenic effect (with 44.03% maximum inhibition) on TA100 strain. Furthermore, it was found that 0.05 mg/plate ethanolic extract showed moderate antimutagenic activity with 25.55% inhibition rate. The highest concentration (0.1 mg/plate) of ethanolic extract of S. annua subsp. showed moderate antimutagenic activity on both Salmonella typhimurium TA98 and TA100 strains with inhibition rates of 20.39 and 22.13%, respectively. No antimutagenic activity on Salmonella typhimurium TA98 and TA100 strains was observed at the lowest doses of both plant species. As a result, it is concluded that S. thirkei and annua subsp. have antimutagenic activity and further studies may be recommended in terms of different parameters.Öğe DETERMINATION OF THE PRESENCE AND ANTIBIOTIC SUSCEPTIBILITY OF FRANCISELLA TULARENSIS IN SPRING WATERS IN DUZCE, TURKEY(Parlar Scientific Publications (P S P), 2017) Dülger, Görkem; Uğraş, Serpil; Dülger, BaşaranThis study aimed to investigate the presence of Francisella tularensis in the spring waters of Duzce. Water samples were collected at regular monthly intervals from 20 different stations between November 2015 and November 2016. Membrane filtration method was used for water sample filtration. In a culture study, glucose cysteine blood agar selective medium was used for F. tularensis isolation. The Tu14 primer, specific to F. tularensis, was used in PCR for molecular identification. F. tularensis was isolated from 2 of the 240 water samples. Antibiotic susceptibility of F. tularensis was determined by disk diffusion method using doxycycline, erythromycin, streptomycin, ciprofloxacin, chloramphenicol, gentamicin, meropenem, tobramycin, tetracycline, nitrofurantoin and imipenem antibiotics.The highest efficacy was demonstrated by Ciprofloxacin.Öğe Evaluating antimicrobial and antioxidant capacity of endemic Phlomis russeliana from Turkey and its antiproliferative effect on Human Caco-2 Cell Lines(Acad Brasileira De Ciencias, 2019) Alpay, Merve; Dülger, Görkem; Şahin, İbrahim E.; Dülger, BaşaranIn this study, the antimicrobial, antioxidant and antitumor activity of ethanol extracts obtained from Phlomis russeliana (Sims.) Lag. ex Benth. (Lamiaceae) were evaluated. Disc diffusion and microdilution methods were used to test the extracts for antimicrobial activity against seven bacteria strains (Bacillus cereus ATCC 7064, Bacillus subtilis ATCC 6633, Staphylococcus aureus ATCC 6538P, Escherichia coli ATCC 10538, Proteus vulgaris ATCC 6899, Salmonella typhimurium CCM 5445 and Pseudomonas aeruginosa ATCC 27853) and four yeast strains (Kluyveromyces fragilis ATCC 8608, Rhodotorula rubra ATCC 70403, Debaryomyces hansenii DSM 70238 and Candida albicans ATCC 10239). Notably, they were more effective against the yeast strains than the bacterial strains. Of the yeast cultures, D. hanseii was among the most susceptible, having an inhibition zone of 16.2 mm with minimum inhibitory concentrations (MICs) and minimum fungicidal concentrations (MFCs) of 64(128)mu g/ml, respectively. For cytotoxic determination, Caco-2 cells were cultured as per ATCC protocol, and were treated with log concentrations (5-80 mg/ml) of P. russeliana. The potency of cell growth inhibition for each extract was expressed as an IC50 value. Moreover, oxidant capacity was evaluated via TOC assay. This product induced antiproliferative activity of 31.33% at 40 mg/nil and 20.96% at 80 mg/ml, without toxic effects on cells, although the oxidant capacity was decreased to 27.06 +/- 0.7 nm in the 80 mg/nilapplied group compared to 47.9 +/- 1.8 mn in the untreated one. Advanced pharmacological studies are needed to further evaluate P. russeliana for distinctive features.Öğe Evaluation of Antimicrobial Activities of Salvia verbenaca(Natural and Engineering Science, 2023) Dülger, Görkem; Dülger, BaşaranThe antimicrobial activity of several extracts and fractions of Salvia verbenaca L. (Lamiaceae) was investigated by disc diffusion and broth microdilution methods against Escherichia coli ATCC 10536, Staphylococcus aureus ATCC 6538P, Klebsiella pneumoniae UC57, Micrococcus luteus La 2971, Micrococcus flavus ATCC 14452, Proteus vulgaris ATCC 8427, Pseudomonas aeruginosa ATCC 27853, Listeria monocytogenes ATCC 19115, Mycobacterium smegmatis CCM 2067, Bacillus cereus ATCC 7064, Bacillus subtilis ATCC 6633, Candida albicans ATCC 10231, Cryptococcus neoformans ATCC 90112, Kluyveromyces fragilis NRRL 2415 and Rhodotorula rubra DSM 70403. The methanol extract, butanol and chloroform fractions have shown potential antimicrobial effects against some bacteria and the yeast cultures tested, with grown inhibition area diameters in the range 10.8 – 22.4 mm, and MIC values between 0.03 and 0.34 µL/mL. The results of the study support the use of the plant in traditional medicine. © 2023, Natural and Engineering Science. All rights reserved.Öğe Exploring the antibiofilm effects on Escherichia coli biofilm associated with colon cancer and anticancer activities on HCT116 cell line of bee products(Taylor & Francis Ltd, 2024) Akkoyunlu, Aysegul; Dülger, GörkemThe association between dysbiotic microbiota biofilm and colon cancer has recently begun to attract attention. In the study, the apitherapeutic effects of bee products (honey, bee venom, royal jelly, pollen, perga and propolis) obtained from the endemic Y & imath;& gbreve;& imath;lca ecotype of Apis mellifera anatoliaca were investigated. Antibiofilm activity were performed by microplate assay using crystal violet staining to measure adherent biofilm biomass of Escherichia coli capable of forming biofilms. Bee venom showed the highest inhibition effect (73.98%) at 50% concentration. Honey, perga and royal jelly reduced biofilm formation by >50% at all concentrations. The antiproliferation effect on the HCT116 colon cancer cell line was investigated with the water-soluble tetrazolium salt-1 assay. After 48 h of honey application at 50% concentration, cell proliferation decreased by 86.51%. The high cytotoxic effects of royal jelly and bee venom are also remarkable. Additionally, apoptotic pathway analysis was performed by ELISA using caspase 3, 8 and 9 enzyme-linked immunosorbent assay kits. All bee products induced a higher expression of caspase 9 compared with caspase 8. Natural products that upregulate caspase proteins are promising therapeutic targets for proliferative diseases.
