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Öğe Antileukemic effects of piperlongumine and alpha lipoic acid combination on Jurkat, MEC1 and NB4 cells in vitro(Medknow Publications & Media Pvt Ltd, 2016) Alpay, Merve; Dikmen, Begüm Yurdakök; Kısmalı, Görkem; Sel, TevhideAim of Study: This research indicated to evaluate the effects of piperlongumine (PL), a biologically active alkaloid, and alpha lipoic acid (ALA), a naturally occurring cofactor existed in multienzyme complexes regulating metabolism on leukemia cells. Excessive production of reactive oxygen species (ROS) can lead to oxidative stress, a state that has been observed in several hematopoietic malignancies, including acute and chronic myeloid leukemias. The importance of the association between oxidative stress and malignancy is not currently clear; however, there is evidence that tumor.derived ROS may promote cell survival, migration and metastasis, proliferation and even drug.resistance depending on the origin of the cancer. Increased oxidative stress in leukemic cells may represent a potential therapeutic target, although there are differing opinions on whether therapeutic strategies should aim to antagonize or further promote oxidative stress in leukemic cells. Materials and Methods: The effects of PL alone (5, 15, 30 M) and in combination (30 M) with ALA (200 M) on Jurkat, NB4 and MEC1 leukemia cell lines were investigated through MTT, caspase-3 and cyclooxygenase-2 (COX-2) activities. Results: Inhibition of COX-2 and the induction of caspase.3 cleavage in Nb4 (acute promyelocytic leukemia) cells were found to be significant following PL application and synergistic effects with combination of ALA (inhibition of COX-2 as 23.74% and 3.55-fold increase of caspase-3). Conclusion: PL and ALA may have a potential value as a therapeutic agent for patients with acute promyelocytic leukemia.Öğe Antimicrobial, Antiproliferative Effects and Docking Studies of Methoxy Group Enriched Coumarin-Chalcone Hybrids(John Wiley and Sons Inc, 2023) Badreddin Musatat, Ahmad; Kılıçcıoğlu, İlker; Kurman, Yener; Dülger, Görkem; Alpay, Merve; Yağcı, Ravza; Atahan, AlparslanMethoxy group enriched eight coumarin-chalcone hybrid derivatives were synthesized. Antimicrobial/ antiproliferative activities were tested against eight human pathogenic microorganisms and four cancer cell lines (AGS, HepG2, MCF-7 and PC-3), respectively. Antimicrobial results showed that most of the compounds were almost more active than used standard antibiotics. Cytotoxicity results showed that 2,3,4-trimethoxyphenyl and thiophene containing structures have promising antiproliferative effects against AGS gastric cell lines with ?5 ?g/ml IC50 values. At the same time, 2,4-dimethoxyphenyl bearing derivative exhibited the lowest IC50 values against HepG2 (?10 ?g/ml) and PC-3 (?5 ?g/ml) cell lines. Particularly, the cell viabilities of MCF-7 cell lines were remarkably inhibited by all the compounds with lower IC50 values. Therefore, molecular docking studies between hybrid ligands and quinone reductase-2 enzyme (regulates in MCF-7 cancer cells) were performed. The results demonstrated that all the derivatives can smoothly interact with interested enzyme in agreement with the experimental results. Finally, ADME parameters were studied to reveal drug-likeness potentials of the coumarin-chalcone hybrids. © 2023 Wiley-VHCA AG, Zurich, Switzerland.Öğe Antioxidant therapy impresses in oxidative stress-induced kidney cells(Comenius Univ, 2017) Alpay, Merve; Kısmalı, Görkem; Meral, Öğünç; Sel, Tevhide; Özmerdivenli, Recep; Pasin, ÖzgeINTRODUCTION: Renal cell cancer (RCC) is not a single entity, but consists of different types of tumors derived from various parts of the nephron (epithelium or renal tubules). It is known that cancer initiation and progression is related to the balance between oxidants and antioxidants directly. Interestingly, advanced stages of cancer such as metastasis, angiogenesis are associated with cell oxidative capacity. Familiar antioxidative substances such as carotenes and vitamin C inhibit oxidation of other molecules during carcinogenesis. They can define the distinction between cancer and normal cells, destroying cancer cells while stabilizing healthy cells. METHODS: apoptotic activities of kidney cells were measured with caspase Elisa kits. DNA laddering test was used to show DNA damage in H2O2 condition. RESULTS: For tumor mechanism, they act as pro-oxidants, producing hydrogen peroxide that attacks the cancer, whereas, in normal conditions they act as protective antioxidants. CONCLUSIONS: The unlike reaction of specific antioxidants should be known at different cell stages. The aim of this study was to assess the antioxidative roles of alpha lipoic acid on kidney cancers during oxidative stress induction (Tab. 1, Fig. 7, Ref 27). Text in PDF www.elis.sk.Öğe Capsaicin inhibits cell proliferation by cytochrome c release in gastric cancer cells(Sage Publications Ltd, 2014) Meral, Öğünç; Alpay, Merve; Kısmalı, Görkem; Kosova, Funda; Çakır, Dilek Ülker; Pekcan, Mert; Sel, TevhideCapsaicin (trans-8-methyl-N-vanillyl-6-nonenamide) is the principal pungent component in hot peppers. The role of capsaicin in carcinogenesis is quite controversial. Although some investigators suspect that capsaicin is a carcinogen, co-carcinogen, or tumor promoter, others have reported that it has chemopreventive and chemotherapeutic effects. The present study aimed to evaluate the cytotoxicity and chemosensitizing activities of capsaicin alone and on 5-flourouracil (5-FU)-treated gastric cancer cells. In this study, the gastric cancer cell line HGC-27 was used and capsaicin used as a chemosensitizer and 5-flourouracil (5-FU) was used as chemotherapeutic. Cytotoxicity and chemosensitizing activities were analyzed with MTT assay; supernatant levels of LDH and glucose were detected as biochemical markers of cell viability; cytochrome c and AIF were evaluated with western blot; and additionally, wound-healing assays were employed. Results suggested that capsaicin had significant anticancer abilities; such capsaicin were capable of causing multifold decreases in the half maximal inhibitory concentration IC50 value of 5-FU. The continuing controversy surrounding consumption or topical application of capsaicin clearly suggests that more well-controlled epidemiologic studies are needed to evaluate the safety and efficacy of capsaicin use. In summary, the present study demonstrated that capsaicin has the potential to be used for treating gastric carcinoma with 5-FU in vitro.Öğe Comparison of serum B12, folate and homocysteine concentrations in children with autism spectrum disorder or attention deficit hyperactivity disorder and healthy controls(Dove Medical Press Ltd, 2019) Yektaş, Çiğdem; Alpay, Merve; Tufan, Ali EvrenObjective: We aimed to investigate the serum concentrations of vitamin B12, folate and homocysteine in children diagnosed with attention deficit hyperactivity disorder (ADHD) or autism spectrum disorder (ASD) and healthy controls. Materials and methods: Serum vitamin B12, folate and homocysteine concentrations were measured in 118 children (48 children diagnosed with ADHD, 35 children diagnosed with ASD and 35 healthy controls). Symptom severity in the ADHD and ASD groups was evaluated by the Childhood Autism Rating Scale and Turgay-DSM-1V-Based Screening and Assessment Scale for Disruptive Behavior Disorders. Multivariate analysis of covariance was used to evaluate the effects of diagnosis and gender on biochemical parameters. Results: The ADHD and ASD groups and the healthy controls differed significantly regarding vitamin B12 and homocysteine concentrations, but not folate levels. Patients with ASD had the lowest vitamin B12 and the highest homocysteine levels. Vitamin B12 levels correlated negatively with hyperactivity and/orimpulsivity and oppositionality symptoms in children with ADHD. There were no relationships between psychometric evaluations and laboratory measurements in children with ASD. Gender did not affect vitamin concentrations. Conclusion: Previous studies found that vitamin B12 was reduced while homocysteine was elevated among patients with ADHD and ASDs. Our results also support those reported previously. Oppositionality and hyperactivity and/orimpulsivity may be related to vitamin B12 and homocysteine levels in children with ADHD. Further studies are required to define the role of these parameters and effects on the etiology and clinical manifestations of ASD and ADHD.Öğe Comparison of Type I Diabetes Frequency in Children with Cesarean and Normal Vaginal Delivery(2020) Şahin, İbrahim Ethem; Hacıoğlu, Ceyhan; Alpay, Merve; Kılıçaslan, ÖnderAim: The effect of type 1 diabetes mellitus over the world is rising day after day. There arecontroversial results that may be related to cesarean delivery that has become widespread inrecent years and the risk of type 1 diabetes mellitus. In this study, we aimed to investigate thefrequency of type 1 diabetes mellitus in children born by cesarean delivery or normal vaginaldelivery, considering that there may be an association between mode of birth way and diabetesmellitus.Material and Methods: The study was organized with 368 children who were diagnosed astype 1 diabetes mellitus between 8-16 ages that applied to the diabetes outpatient clinic in2019. The patients were grouped according to the mode of delivery. Descriptive data such asage, gender, HBA1c, and BMI were evaluated retrospectively.Results: The children with type 1 diabetes mellitus, cesarean delivery show 33.2% more thannormal vaginal delivery (p<0.001). While 9.0% of children with cesarean delivery hadmother's diabetes mellitus, this rate was 5.7% in children with normal vaginal delivery.Similarly, 5.3% of children with cesarean delivery had diabetes mellitus in their father, whilethis rate was 8.9% in children with normal vaginal delivery.Conclusion: According to the results of this study, cesarean delivery may have a significanteffect on the risk for type 1 diabetes mellitus in childhood either adolescence. Even if parentswith diabetes were not included, it was found that cesarean delivery have meaningful relationby diabetes mellitus stimulation. Regarding this, further studies are needed.Öğe COVID-19 Hastalarında TNF-? Düşüşünü Etkileyen Faktörler(2024) Gülhan, Muhammet; Alpay, Merve; Yıldız, Şule; Yaşlı, Nevra Ezgi; Acat, Murat; Kahraman, Gözde; Arbak, PeriAmaç: Tümör nekrozis faktör-alfa (TNF-?) hücrenin hayatta kalması, farklılaşması ve çoğalması da dahil çeşitli işlevlere sahiptir. TNF-?, COVID-19 enfeksiyonu sırasında ortaya çıkan sitokin fırtınası sırasında hücre ölümünü etkileyebilir. Bu çalışmada COVID-19 hastalarında TNF-? düşüşüne etki eden faktörleri araştırmak amaçlandı. Gereç ve Yöntemler: Çalışmaya COVID-19 enfeksiyonu olan toplam 44 kişi dahil edildi. Kan sayımı, biyokimyasal incelemeler ve D-dimer incelemeleri yapıldı. Hastaların ek hastalıkları ve başlangıç semptomları kaydedildi. TNF-?, CRP ve ferritin değerleri yatışının 1. ve 7. günlerinde iki kez ölçüldü. Bu değerlerdeki değişim komorbiditelere, başlangıç semptomlarına ve steroid kullanımına göre incelendi. Bulgular: Toplam 44 hastanın %56,8'i erkek (25/44) ve %43,2'si kadındı (19/44). TNF-? düşüşü diyabetik olmayan hastalarda istatistiksel olarak anlamlı bulunurken, diyabetik hastalarda TNF-? düşüşü anlamsız bulundu. CRP düşüşü hem diyabetik hem de diyabetik olmayan hastalarda istatistiksel olarak anlamlı bulunurken, ferritin düşüşü hem diyabetik hem de diyabetik olmayan hastalarda anlamsız bulundu. Akciğer hastalığı olmayanlarda TNF-? ve CRP istatistiksel olarak anlamlı düzeyde düşerken, akciğer hastalığı olanlarda hem TNF-? hem de CRP düşüşlerinin anlamlı olmadığı görüldü. TNF-? ve CRP'deki azalma steroid ile tedavi edilmeyenlerde anlamlı değilken, TNF-? ve CRP'deki azalma steroid tedavisi alanlarda istatistiksel olarak anlamlıydı. Sonuç: Steroidler TNF-? düzeyini düşürürler. Akciğer hastalığı ve diyabeti olanlarda TNF-? düşüşü anlamlı değildir.Öğe COVID-19 Olası/Kesin Tanılı Hastalarda Serbest Radikal Ve Antioksidan Enzim Düzeylerinin Değerlendirilmesi(2023) Davran, Fatih; Hacıoğlu, Ceyhan; Alpay, Merve; Yıldız Gülhan, Pınar; Öztürk, C. Elif; Ince, Nevin; Unlu, Elif NisaAmaç: Koronavirüs hastalığı 2019 (COVID-19), küresel halk sağlığını tehdit eden ve bir pandemiye yol açan karmaşık bir hastalık olmasına rağmen, hastalığın patogenezinde yer alan pro-oksidan/oksidan dengesizliğinin katkıları yeterince araştırılmamıştır. Bu nedenle, bu çalışmanın amacı, COVID-19 hastalarında sigara kullanımının oksidatif stres ve antioksidan savunma sistemi biyobelirteçleri üzerindeki etkilerini değerlendirmektir. Gereç ve Yöntemler: Çalışmaya katılan kişiler 4 gruba ayrıldı: (Kontrol grubu) COVID-19 tanısı olmayan ve sigara kullanmayan 45 sağlıklı birey, (Grup 2) COVID-19 tanısı olmayan ve sigara kullanan 45 birey, (Grup 3) COVID-19 tanılı ve sigara kullanmayan 45 hasta, (Grup 4) COVID-19 tanılı ve sigara kullanan 45 hasta. Katılımcıların serumlarındaki D-dimer, nitrik oksit (NO), glutatyon redüktaz (GR), süperoksit dismutaz (SOD), katalaz (CAT), malondialdehid (MDA), total antioksidan seviyeleri (TAS) ve total oksidan seviyeleri (TOS) seviyelerini ticari kitler kullanarak ELİSA yöntemine göre analiz edildi. Bulgular: COVID-19 tanısı alan kişilerdeki D-dimer seviyelerinde anlamlı bir artış söz konusudur (pÖğe Decreased Oxidative Stress Markers in Patients with Active and Generalized Vitiligo(Duzce Univ, Fac Medicine, 2022) Ozcan, Yunus; Karagun, Ebru; Alpay, MerveObjective: Oxidative stress has been shown to play a role in the pathophysiology of several diseases, making it a popular yet contentious research area. There is some evidence that selective melanocyte destruction may have developed in vitiligo patients as a result of elevated oxidative stress. The purpose of this study is to investigate the impact of oxidative stress on lipid, protein, and nucleic acid metabolism in vitiligo patients. Methods: We used ELISA method to measure serum oxidative stress markers in patients with generalized vitiligo who had newly formed lesions in the previous three months but had not been treated, as well as healthy controls. Malondialdehyde (MDA), 2,4-dinitrophenyl hydrazone (DNPH), 8-hydroxy-2'-deoxyguanosine (8-OHdG), and uncoupling protein 2 (UCP2) levels were measured to assess the influence of reactive oxygen derivatives on lipid, protein, nucleic acid metabolism, and mitochondria, respectively. Results: The study included 84 participants, including 64 active generalized vitiligo patients and 20 healthy controls with similar age and gender distribution. In the serum of vitiligo patients, we detected significantly lower levels of MDA (ng/mL, mean +/- SD=12 +/- 19; 33.4 +/- 35.9), DNPH (ng/mL, mean +/- SD=2 +/- 3.1; 6 +/- 7.4), 8-OHdG (ng/mL, mean +/- SD=11.7 +/- 17.9; 32.7 +/- 37) and UCP2 (ng/mL, mean +/- SD=8.7 +/- 13.7; 21.5 +/- 28.4. Conclusions: Although there is significant evidence that oxidative stress plays a role in the pathophysiology of vitiligo, the studies should be interpreted cautiously due to the heterogeneity in the methodology, complexity of the oxidative stress pathways, and potential publication bias. Large-scale studies using a standardized methodology are required to determine how significant oxidative stress is in the core pathophysiology of vitiligo and which pathways it primarily affects.Öğe The effect of hypoxic and normoxic cerebral oximetry levels and oxidative stress on cognitive functions after carotid endarterectomy(Lippincott Williams & Wilkins, 2021) Yorulmaz, İlknur Suidiye; Karaca, Okay Guven; Alpay, Merve; Bal, Melih[Bastract Not Available]Öğe The effect of piracetam and hyperbaric oxygen application on return of nerve damage in rats with a model of sciatic nerve damage(Lippincott Williams & Wilkins, 2021) Yorulmaz, İlknur Suidiye; Canoğlu, Umut Caner; Gamsızkan, Mehmet; Iskender, Abdulkadir; Alpay, Merve; Sungur, Mehmet Ali[Bastract Not Available]Öğe Effect of various irrigation solutions on cytokine expression of human gingival fibroblast: in vitro study(2019) Aydın, Zeliha Uğur; Akpınar, Kerem Engin; Hepokur, Ceylan; Alpay, Merve; Altunbaş, DemetAim: The aim of this study is to investigate the release of IL-1,TNF- ? and VEGF following administration of NaOCl, propolisand chitosan solutions on human gingival fibroblasts.Materials and Methods: This study was conducted on humanfibroblast by cell culture and evaluation of the direct effectof various solutions on the cultured cells. The release ofpro-inflammatory interleukin (IL), tumor necrosis factor (TNF)? and vascular endothelial growth factor (VEGF) on fibroblastwas analyzed after administration of irrigation solutions. Logconcentrations of NaOCl, Propolis and Chitosan effects oncells were measured by colorimetric method.Results: IL-1 and TNF-? secretion levels decreased duringpropolis and chitosan applications, which are natural products.It was also found that the propolis increased VEGF secretionmore than the other materials.Conclusions: The results of this study suggest that propolisand chitosan may contribute to the recovery of periapical tissuesvia anti-inflammatory cytokines level secreted during theinflammatory process. It is important to search for the biologicaleffect of the materials in contact with the direct or indirectcause of the surrounding tissues during the endodontic treatment.Öğe The effects of subconjunctival bevacizumab, ranibizumab, and aflibercept on corneal neovascularization(Sage Publications Ltd, 2022) Eski, Mehmet Tahir; Teberik, Kuddusi; Oltulu, Pembe; Ankaralı, Handan; Kaya, Murat; Alpay, MervePurpose To investigate the effects of subconjunctival bevacizumab, ranibizumab, and aflibercept in an experimental corneal neovascularization model. Materials and methods The eyes of 24 rats were chemically cauterized and randomly divided into four groups: bevacizumab group (0.05 mL/1.25 mg bevacizumab), ranibizumab group (0.05 mL/0.5 mg ranibizumab), aflibercept group (0.05 mL/1.25 mg aflibercept), and control group (0.05 mL saline solution). Plasma vascular endothelial growth factor (VEGF) levels were among the major measurement outcomes to assess corneal neovascularization. The collected plasmas were analyzed using the SIGMA RAB0511 Rat VEGF Elisa kit. The PCR technique and VEGF amplification procedures were used for molecular analysis. Each cornea was removed and histologically examined on day 21. Corneal images were evaluated by image analyzer software. Results In the post-injection period, the number of major corneal arteries decreased significantly in the injection groups when compared to the control group (p = 0.037), but no statistically significant differences were noted among the injection groups (p > 0.05). The aflibercept group had the lowest area of neovascularization. Immunohistochemical staining revealed substantially lower VEGF percentages in neovascularized arteries of the injection groups than the control group (p = 0.015). In TUNEL staining, the mean TUNEL value (number/1hpf) was substantially greater in the control group than in the injection groups, but the mean TUNEL values were similar between the injection groups (p = 0.019, p > 0.05, respectively). No statistically significant differences were observed between the groups in terms of corneal surface area with increased cellularity, edema, and inflammation (p = 0.263). The mean plasma VEGF concentration in the control group was statistically greater than those in the injection groups (p = 0.001). Conclusion Subconjunctival bevacizumab, ranibizumab, and aflibercept crossed the blood and seemed to be effective in inhibiting corneal neovascularization without causing epitheliopathy in an experimental rat model compared to the controls. However, no significant results were noted between these three anti-VEGF molecules.Öğe The Effects of Vitamin E on Antioxidant Enzyme Activity in HepG2 Cells(Kafkas Univ, Veteriner Fakultesi Dergisi, 2016) Balkan, Burcu Menekşe; Kısmalı, Görkem; Alpay, Merve; Sayıner, Serkan; Turan, Deniz; Balkan, Ali Burak; Sel, TevhideIt is aimed to investigate the effect of vitamin E, powerful antioxidant (alpha-tocopherol succinate) on antioxidant enzyme activities in hepatocellular carcinoma (HepG2) cells. The hepatocellular carcinoma cell line HepG2 was used and the cells were cultured in the absence (control) or presence of different dose of vitamin E (50 mM, 50 mu M and 10 mu M vitamin E) for 24 h. The effect of vitamin E (alpha-tocopherol succinate) on catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx) enzyme activities in hepatocarcinoma cells were measured by spectrophotometry. A significant decrease in GPx activity was detected in 50 mM vitamin E treated HepG2 cells. However a significant decrease occurred in 10 mu M and 50 mu M vitamin E applied HepG2 cells. SOD activity in study groups were lower than in control cells. In addition to this, the decrease in SOD activity in 50 mM vitamin E applied cells was significant. CAT enzyme activity in 50 mu m vitamin E applied HepG2 cells was higher and, in 10 mu M and 50 mM vitamin E applied HepG2 cells were lower than in control group. It was determined that vitamin E has a dose-dependent effect on antioxidant enzyme activity in HepG2 cells.Öğe The Effects of Vitamin E on Antioxidant Enzyme Activity in HepG2 Cells [1](2016) Balkan, Burcu Menekşe; Kısmalı, Görkem; Alpay, Merve; Sayıner, Serkan; Turan, Deniz; Balkan, Ali Burak; Sel, TevhideGüçlü bir antioksidan olan vitamin E'nin (alfa-tokoferolsüksinat) HepG2 hücrelerinde antioksidan enzim aktiviteleri üzerine etkisinin araştırılması amaçlanmıştır. Çalışma materyali olarak HepG2 hücre hattı kullanılmıştır. Vitamin E uygulanan hücreler çalışma grubunu vitamin E uygulaması yapılmayan hücreler kontrol grubunu oluşturmuştur. Çalışma grubu hücrelerine 10 µM, 50 µM, 50 mM dozlarda vitamin E uygulaması yapılarak, 24 saat sonunda HepG2 hücrelerinde antioksidan enzimlerden katalaz (CAT), süperoksitdismutaz (SOD) ve glutasyonperoksidaz (GPx) aktiviteleri spektrofotometrik olarak ölçülmüştür. 50 mM vitamin E uygulanan HepG2 hücrelerinde GPx enzim aktivitesinde anlamlı bir artış saptanmıştır. Ancak, 10 µM ve 50 µM vitamin E uygulanan HepG2 hücrelerinde ise anlamlı bir azalma meydana gelmiştir. Vitamin E uygulaması yapılan hücrelerdeki SOD aktivitesi vitamin E uygulaması yapılmayan kontrol grubuna göre daha düşük ölçülürken, 50 µM vitamin E uygulanan HepG2 hücrelerinde SOD aktivitesinde anlamlı azalma tespit edilmiştir. 50 µM vitamin E uygulanan HepG2 hücrelerindeki CAT enzim aktivitesi kontrol grubuna göre daha yüksek bulunurken, 10 ?M ve 50 mM vitamin E uygulanan hücrelerdeki CAT enzim aktivitesi kontrol grubuna göre daha düşük tespit edilmiştir. Vitamin E'nin HepG2 hücrelerinde antioksidan enzim aktiviteleri üzerinde doz-bağımlı etkisinin olduğu belirlenmiştirÖğe Evaluating antimicrobial and antioxidant capacity of endemic Phlomis russeliana from Turkey and its antiproliferative effect on Human Caco-2 Cell Lines(Acad Brasileira De Ciencias, 2019) Alpay, Merve; Dülger, Görkem; Şahin, İbrahim E.; Dülger, BaşaranIn this study, the antimicrobial, antioxidant and antitumor activity of ethanol extracts obtained from Phlomis russeliana (Sims.) Lag. ex Benth. (Lamiaceae) were evaluated. Disc diffusion and microdilution methods were used to test the extracts for antimicrobial activity against seven bacteria strains (Bacillus cereus ATCC 7064, Bacillus subtilis ATCC 6633, Staphylococcus aureus ATCC 6538P, Escherichia coli ATCC 10538, Proteus vulgaris ATCC 6899, Salmonella typhimurium CCM 5445 and Pseudomonas aeruginosa ATCC 27853) and four yeast strains (Kluyveromyces fragilis ATCC 8608, Rhodotorula rubra ATCC 70403, Debaryomyces hansenii DSM 70238 and Candida albicans ATCC 10239). Notably, they were more effective against the yeast strains than the bacterial strains. Of the yeast cultures, D. hanseii was among the most susceptible, having an inhibition zone of 16.2 mm with minimum inhibitory concentrations (MICs) and minimum fungicidal concentrations (MFCs) of 64(128)mu g/ml, respectively. For cytotoxic determination, Caco-2 cells were cultured as per ATCC protocol, and were treated with log concentrations (5-80 mg/ml) of P. russeliana. The potency of cell growth inhibition for each extract was expressed as an IC50 value. Moreover, oxidant capacity was evaluated via TOC assay. This product induced antiproliferative activity of 31.33% at 40 mg/nil and 20.96% at 80 mg/ml, without toxic effects on cells, although the oxidant capacity was decreased to 27.06 +/- 0.7 nm in the 80 mg/nilapplied group compared to 47.9 +/- 1.8 mn in the untreated one. Advanced pharmacological studies are needed to further evaluate P. russeliana for distinctive features.Öğe From the Laboratory to the Clinic: Molecular Treatment of Heart Failure(Duzce Univ, Fac Medicine, 2024) Alagoz, Mehmet; Alpay, MerveCoronary and cardiovascular diseases are the leading cause of death today, with heart failure being among the primary culprits. Heart failure can occur as a result of many diseases, so research in this area is important in terms of clinical outcomes and treatment. Histopathology of heart failure includes cardiac hypertrophy, inflammation, angiogenesis, and apoptosis pathways. The issue of elucidating the pathology of heart failure is still an area of active research. In advanced heart failure, the typical management strategy is medical treatment, mechanical ventricular support devices, and heart transplantation. Heart failure, which occurs with modifiable and non -modifiable risk factors, can be controlled with both nonpharmacological and pharmacological treatment applications. It is especially important to focus on new treatment methods and introduce them to the clinic. Although they are all not yet used in clinics, many studies have yielded promising results with molecular treatment options for heart failure prevention. Studies in animals have shown that heart failure stops proceeding when angiogenesis is induced. Promising results have also been achieved with stem cell therapy, but these may not be implementable for years. It is expected that studies following phases 1 and 2, of the studies which had positive results in the treatment of heart failure, will be conducted and applied in the daily treatment practice.Öğe GSM-like radiofrequency exposure induces apoptosis via caspase-dependent pathway in infant rabbits(Comenius Univ, 2016) Meral, Öğünç; Özgür, Elçin; Kısmalı, Görkem; Güler, Gülnur; Alpay, Merve; Sel, Tevhide; Seyhan, NesrinBACKGROUND: There have been several Radio Frequency (RF) field researches on various populations and groups of different ages in recent years. However, the most important group for research has been declared as the pregnant women and their babies. OBJECTIVE: The aim of the study was to analyse the effect on apoptotic factors of RF fields on newborn rabbit liver tissues. MATERIALS AND METHODS: Cytochrome c and AIF (Apoptosis Inducing Factor) levels were measured by western blot and caspase 1, 3 and 9 activities were measured by colorimetric method. RESULTS: Cytochrome c and AIF levels were not altered, but all caspase activities were increased in female infant rabbits that exposed to 1800 MHz GSM-like RF signals when they reached 1 month of age and caspase 1 and caspase 3 levels were decreased in male infant rabbits that exposed to 1800 MHz GSM-like RF signals between 15th and 22nd days of the gestational period. Results showed that 1800 MHz GSM-like RF exposure might lead to apoptosis in infant rabbit's liver tissues. CONCLUSION: According to the results, we suggest that postnatal RF exposure causes caspase dependent apoptosis in female infant rabbits liver tissues (Tab. 1, Fig. 2, Ref. 27). Text in PDF www.elis.sk.Öğe Heightened Serum Mitochondrial Biomarkers; FGF21 and NOS in Pediatric Anemia and a Negative Correlation between GDF15 and Serum Ferritin(Mdpi, 2024) Cakmak, Hatice Mine; Alpay, Merve; Mahdizadeh, Cansu; Ozalp, Seray Cevikel; Turay, Sevim; Ozde, Sukriye; Kocabay, KenanObjective: Mitochondrial dysfunction is closely linked to chronic disorders. This study aims to explore the correlation between pediatric anemia and mitochondrial markers, specifically fibroblast growth factor 21 (FGF21), growth/differentiation factor 15 (GDF-15), and nitric oxide synthase (eNOS). Method: This study included 66 children, with 34 diagnosed with anemia and 32 in the healthy control group. Statistically significant biomarkers were determined through cutoff levels. Results: Among the participants, 34 children were classified as anemic, while 32 were categorized as healthy. The study revealed that FGF21 levels >= 0.745 pg/mL and eNOS levels >= 1.265 mu g/mL predicted anemia. Hemoglobin levels exhibited a negative correlation with FGF21 (r = -0.381; p = 0.002) and eNOS levels (r = -0.462; p < 0.001). Furthermore, a significant negative correlation was observed between GDF-15 and ferritin (r = -0.311; p = 0.019), while eNOS levels correlated positively with folate (r = 0.313; p = 0.019). Conclusions: Anemia induced elevated mitochondrial biomarkers; FGF21 and eNOS levels. The findings suggest that the long-term ramifications of anemia in childhood may be associated with mitochondrial dysfunction.Öğe In Vitro Effects of Phthalate Mixtures on Colorectal Adenocarcinoma Cell Lines(Begell House Inc, 2015) Dikmen, Begüm Yurdakok; Alpay, Merve; Kısmalı, Görkem; Filazi, Ayhan; Kuzukıran, Özgür; Şireli, Ufuk TanselAmong endocrine-disrupting chemicals, phthalates are an important concern because of their widespread exposure in humans and environmental contamination. Even though the use of some phthalates has been restricted for toys, some plastics, and food contact materials, exposure to the mixture of these contaminants at very low concentrations in various matrices are still being reported. In the current research, the effects of the mixture of some phthalates were studied. Di-n-butyl phthalate (DBP), n-butyl benzyl phthalate (BBP), di-2-ethylhexyl phthalate (DEHP), diisononyl phthalate (DiNP), di-n-octyl phthalate (DNOP), and diisodecyl phthalate (DiDP) were tested on two colorectal adenocarcinoma cell lines; DLD-1 and HT29 were studied as described before. Cells were treated with increasing log concentrations (0.33 ppt to 33.33 ppb) of the phthalate mixture, cell viability/proliferation was measured by MTT and staining with neutral red and crystal violet; lactate dehydrogenase (LDH) activity was measured following 24-h exposure. Cell viability/proliferation increased from phthalate treatment at concentrations less than 33.33 ppt. The phthalate mixture induced increases in HT29 proliferation of 10.94% at 33.33 ppt and 60.87% at 3.33 ppt, whereas this proliferation relation at lower concentrations was not found for DLD1 cells. The present study demonstrates preliminary information regarding the low dose induction of proliferation of the cancer cells by phthalate mixtures. Because non-monotonic dose responses are still being debated, further studies are required to re-evaluate the reference doses defined by governments for phthalates.
