Morphometric and mtDNA Variability Reveals Beekeeping Influences on Duzce Honeybee Populations
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Date
2023
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Kahramanmaras Sutcu Imam Univ Rektorlugu
Access Rights
info:eu-repo/semantics/closedAccess
Abstract
In this study, the variability of Duzce honey bee populations using morphometric, restriction fragment length polymorphism and sequence analysis of five different regions of mitochondrial DNA were studied. 1440 worker honey bee samples were collected from colonies in distant apiaries located eight district of Duzce provinces. Worker bees were dissected and twelve morphometric characters related to length of proboscis, legs and front wings of samples were measured. A mitochondrial DNA fragment containing non-coding CoxI-CoxII, 16srDNA, ND5, Cytb and CoxI regions were amplified and analyzed with nineteen different restriction endonuclease enzymes. Non-coding CoxI-CoxII gene was digested by DraI, XbaI ve HinfI enzymes and revealed two and three different haplotypes with these enzymes respectively. There were no variation in SspI, XhoI, HinfI and DraI restriction pattern of CoxI gene region of mtDNA. 16srDNA with HincII and SspI revealed two different types for each enzymes. Cytb was revealed two types of patterns with DraI, although ND5 gene revealed no variation for SspI, DraI ve HincII enzymes. Some samples, which were shown variation by RFLP were subjected to sequence analysis for each mitochondrial DNA fargment. Consequently genetic analysis revealed a compatible variation with morphometric analysis. Surprisingly, genetic introgression to common and unique haplotypes was determined due to foreign queen bee
Description
Keywords
Honey bee, mtDNA, Morphometry, PCR-RFLP, Sequencing, Apis-Mellifera L., Mitochondrial-Dna Variation, A. M. Caucasica, Phylogenetic-Relationships, Morphological Characters, Bees Hymenoptera, Important Races, Rflp Analysis, Turkish, Anatoliaca
Journal or Series
Ksu Tarim Ve Doga Dergisi-Ksu Journal of Agriculture And Nature
WoS Q Value
Q4
Scopus Q Value
Volume
26
Issue
4
