Sıçanlarda akut ve kronik uygulanan harmalinin penisilin ile oluşturulmuş epileptiform aktivite üzerine etkisi
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Dosyalar
Tarih
2019
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Düzce Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Bu çalışmada harmalinin kısa ve uzun süreli olarak uygulanmasının sıçanlarda penisilin G ile oluşturulan deneysel epilepsi modeli üzerindeki etkisini elektrofizyolojik olarak araştırıldı. Çalışmada 84 yetişkin erkek Wistar sıçan kullanıldı. Sıçanlar iki gruba ayrıldıktan sonra her bir grup sham, kontrol (penisilin), sadece harmalin ve 10, 50 ve 100 mg/kg dozlarda harmalin grupları olarak 6 farklı alt gruba ayrıldı. Kayıtlardan elde edilen elektrokortikografik veriler PowerLab Chart v.8 yazılım programı ile analiz edildi. Aynı zamanda ilk epileptiform aktivitenin başlama latensi, epileptiform aktivitenin diken dalga sıklığı ve diken dalga genliği istatistiksel olarak analiz edildi. Ayrıca harmalinin antioksidan mekanizması üzerindeki etkilerini tespit etmek amacıyla süperoksit dismutaz (SOD), katalaz (CAT), glutatyon peroksidaz (GPx) ve glutatyon redüktaz (GR) miktarları serumda ELISA metodu ile belirlendi. İstatistiksel analizler one way Anova testi ile incelendi. Her iki grupta doza bağlı olarak nöbete başlama zamanını kontrol grubuna göre istatistiksel olarak artmıştır (p<0.05). Kısa süreli harmalin uygulanan grupta penislin G ile oluşturulan epilepsili ratlarda antiepileptik etki kontrol ve diğer gruplara göre istatistiksel olarak anlamlı bulundu (p<0.05). Ayrıca harmalinin uzun süreli uygulamasının antioksidan kapasitesi, kontrol ve diğer gruplara göre istatistiksel olarak anlamlı bulundu (p<0.05). Sunulan çalışmada monoaminoksidaz inhibitörü olan harmalinin ratlarda penisilinle oluşturulmuş deneysel epilepsi modelinde uygulanarak antiepileptik olduğu ve gelecekte antiepileptik olarak kullanılabileceği öngörülmektedir.
In this study, the effect of short and long term administration of harmaline on penicillin G induced experimental epilepsy model in rats was investigated electrophysiologically. 84 adult male Wistar rats were used in the study. After the rats were divided into two groups, each group was divided into 6 different subgroups as sham, control (penicillin), only harmaline and 10, 50 and 100 mg / kg doses. Electrocorticographic data obtained from the records were analyzed with PowerLab Chart v.8 software program. At the same time, latency of onset of first epileptiform activity, spike wave frequency and spike wave amplitude of epileptiform activity were analyzed statistically. In addition, the amount of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione reductase (GR) were determined by ELISA method in order to determine the effects of harmaline on antioxidant mechanism. Statistical analysis was performed by one way Anova test. In both groups, the time to onset of seizure was increased statistically compared to the control group (p <0.05). Antiepileptic effect was found to be statistically significant in penicillin G induced epileptiform activity in rats with the short term harmaline group compared to the control and other groups (p <0.05). In addition, antioxidant capacity of long term administration of harmaline was statistically significant compared to control and other groups (p <0.05). In the present study, it is suggested that the harmaline, a monoaminoxidase inhibitor, is antiepileptic in the experimental epilepsy model created with penicillin G in rats and may be used as antiepileptic in the future.
In this study, the effect of short and long term administration of harmaline on penicillin G induced experimental epilepsy model in rats was investigated electrophysiologically. 84 adult male Wistar rats were used in the study. After the rats were divided into two groups, each group was divided into 6 different subgroups as sham, control (penicillin), only harmaline and 10, 50 and 100 mg / kg doses. Electrocorticographic data obtained from the records were analyzed with PowerLab Chart v.8 software program. At the same time, latency of onset of first epileptiform activity, spike wave frequency and spike wave amplitude of epileptiform activity were analyzed statistically. In addition, the amount of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione reductase (GR) were determined by ELISA method in order to determine the effects of harmaline on antioxidant mechanism. Statistical analysis was performed by one way Anova test. In both groups, the time to onset of seizure was increased statistically compared to the control group (p <0.05). Antiepileptic effect was found to be statistically significant in penicillin G induced epileptiform activity in rats with the short term harmaline group compared to the control and other groups (p <0.05). In addition, antioxidant capacity of long term administration of harmaline was statistically significant compared to control and other groups (p <0.05). In the present study, it is suggested that the harmaline, a monoaminoxidase inhibitor, is antiepileptic in the experimental epilepsy model created with penicillin G in rats and may be used as antiepileptic in the future.
Açıklama
YÖK Tez No: 621657
Anahtar Kelimeler
Fizyoloji, Physiology
