GRGDS-conjugated and curcumin-loaded magnetic polymeric nanoparticles for the hyperthermia treatment of glioblastoma cells

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dc.authoridSenturk, Fatih/0000-0002-2436-3362
dc.authoridCakmak, Soner/0000-0003-2245-8322
dc.authorwosidSenturk, Fatih/AAR-9444-2021
dc.authorwosidCakmak, Soner/I-4478-2015
dc.contributor.authorSenturk, Fatih
dc.contributor.authorCakmak, Soner
dc.contributor.authorKocum, Ismail Cengiz
dc.contributor.authorGumusderelioglu, Menemse
dc.contributor.authorOzturk, Goknur Guler
dc.date.accessioned2021-12-01T18:50:09Z
dc.date.available2021-12-01T18:50:09Z
dc.date.issued2021
dc.department[Belirlenecek]en_US
dc.description.abstractThermally responsive and ligand-mediated drug delivery systems have the potential to improve the treatment of brain tumors, especially, most lethal one, glioblastoma multiform (GBM). Magnetic nanoparticle-mediated hyperthermia becomes one of the most promising alternative therapy for GBM treatment in cases where localized heating and targeted delivery of a therapeutic drug can be achieved on the tumor site. In this study, it is aimed to increase the therapeutic efficiency of multi-functionalized nanoparticles (NPs) in combination with radiofrequency hyperthermia (RF-HT) on GBM cells. For this purpose, firstly, a low-cost and portable home-built RFHT system suitable for in vitro/in vivo studies was successfully implemented and tested at 13.56 MHz frequency with power up to approximately 400 W. Subsequently, the highly monodispersed superparamagnetic iron oxide nanoparticles (SPIONs), which could interact with the RF magnetic field, were synthesized with the mean particle size of 5.6 +/- 0.9 nm. The obtained SPIONs were coated with poly (lactic-co-glycolic acid)-poly (ethylene glycol) di-block copolymer (PLGA-b-PEG). Most of the SPIONs were uniformly distributed in such a well-defined spherical-shaped polymeric NP. Moreover, curcumin (Cur), a potential agent for GBM treatment, was loaded into the magnetic polymeric nanoparticles (m-PNPs) with a loading capacity of 8% (w/w, Cur/NPs) and a mean diameter of Cur-loaded m-PNPs (Cur-m-PNPs) was 142 +/- 70 nm. To increase cellular uptake and targeting ability of NPs, glycine-arginine-glycine-aspartic acid-serine (GRGDS) peptide was immobilized on the Cur-m-PNPs and the amount of GRGDS was detected as 37 mu g/mg NPs. In vitro cytotoxicity studies revealed that the presence of GRGDS on Cur-m-PNPs (GRGDS-Cur-m-PNPs) improved the cytotoxic efficiency of Cur-m-PNPs by 6-fold in GBM-cells for all incubation times (24, 48 and 72 h). Furthermore, NPs with RF treatment exhibited higher antitumor activity than that of NPs without RF on GBM cells. This result may be attributed to the thermal (SPIONs) or non thermal (cellular membrane) effects or both of them on cells. Overall, this study showed that RF-HT in combination with GRGDS-Cur-m-PNPs could provide a feasible approach to improve GBM treatment.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [SBAG-118S027, SBAG119S137]en_US
dc.description.sponsorshipThis study was supported by the Scientific and Technological Research Council of Turkey (TUBITAK) , Grant no: SBAG-118S027 and SBAG119S137. We are sincerely grateful for the assistance of the Cell and Tissue Engineering Research Group at Hacettepe University. We are also thankful to Dr. Anl Sera Cakmak for the support of cell culture studies.en_US
dc.identifier.doi10.1016/j.colsurfa.2021.126648
dc.identifier.issn0927-7757
dc.identifier.issn1873-4359
dc.identifier.urihttps://doi.org/10.1016/j.colsurfa.2021.126648
dc.identifier.urihttps://hdl.handle.net/20.500.12684/10834
dc.identifier.volume622en_US
dc.identifier.wosWOS:000657418600004en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofColloids And Surfaces A-Physicochemical And Engineering Aspectsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectGlioblastomaen_US
dc.subjectRF hyperthermiaen_US
dc.subjectGRGDSen_US
dc.subjectCurcuminen_US
dc.subjectMagnetic nanoparticlesen_US
dc.subjectTumor-Treating Fieldsen_US
dc.subjectIron-Oxide Nanoparticlesen_US
dc.subjectPlga Nanoparticlesen_US
dc.subjectDrug-Deliveryen_US
dc.subjectIn-Vitroen_US
dc.subjectCanceren_US
dc.subjectTherapyen_US
dc.subjectTemozolomideen_US
dc.subjectEfficacyen_US
dc.subjectMechanismen_US
dc.titleGRGDS-conjugated and curcumin-loaded magnetic polymeric nanoparticles for the hyperthermia treatment of glioblastoma cellsen_US
dc.typeArticleen_US

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