Investigation of the relationship between fluconazole susceptibility, proteinase activity and ERG11-SAP2 Expression in Candida albicans strains isolated from clinical samples

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dc.contributor.authorEge, Nagihan
dc.contributor.authorOksuz, S.
dc.contributor.authorAkbaş, Emel
dc.contributor.authorÇalişkan, Emel
dc.contributor.authorSungur, Mehmet Ali
dc.date.accessioned2025-10-11T20:45:30Z
dc.date.available2025-10-11T20:45:30Z
dc.date.issued2024
dc.departmentDüzce Üniversitesien_US
dc.description.abstractBackground and Purpose: Candida albicans is currently recognised as an opportunistic pathogen that can cause many invasive infections. Resistance mechanisms and fungal virulence factors play an important role in the effectiveness of treatment. The aim of this study was to investigate the relationship between fluconazole resistance, proteinase activity and ERG11 (sterol 14-demethylase)- SAP2 (secreted aspartic protease 2) gene expression levels in C.albicans strains. Materials and Methods: Candida albicans strains isolated from patient samples sent to Medical Microbiology laboratory of Düzce University from various clinics were included in the study. Fluconazole susceptibilities of the isolates were determined by broth microdilution method. The increase in fluconazole MIC values at 48 hours and proteinase activities of the isolates were analysed. ERG11 and SAP2 gene expression levels were measured by real time qPCR. Results: Fluconazole resistance rate was found to be 3.14% in 127 C. albicans strains. A moderate positive correlation was found between ERG11 and SAP2 values (p=0.029, r:0.655, p<0.001). There was no correlation between SAP2/ERG11 expression levels and fluconazole resistance. Proteinase positivity was detected in 81.1%, of 127 strains and no statistically significant correlation was found between proteinase activities and SAP2/ERG11 expression levels. While there was a statistically significant relationship between ERG11 expression levels and 48th hour MIC elevation, there was no statistically significant relationship between SAP2 levels and 48th hour MIC elevation. Conclusion: In addition to the moderate positive correlation between ERG11 and SAP2 values, a significant correlation was found between ERG11 expression and fluconazole tolerance. © 2025 Elsevier B.V., All rights reserved.en_US
dc.identifier.doi10.22034/cmm.2024.345311.1585
dc.identifier.issn2423-3420
dc.identifier.issn2423-3439
dc.identifier.scopus2-s2.0-105007532857en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.urihttps://doi.org/10.22034/cmm.2024.345311.1585
dc.identifier.urihttps://hdl.handle.net/20.500.12684/21361
dc.identifier.volume10en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherMazandaran University of Medical Sciencesen_US
dc.relation.ispartofCurrent Medical Mycologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmzKA_Scopus_20250911
dc.subjectCandida Albicansen_US
dc.subjectFluconazoleen_US
dc.subjectProteinaseen_US
dc.subjectToleranceen_US
dc.subjectAspartic Proteinaseen_US
dc.subjectFluconazoleen_US
dc.subjectProteinaseen_US
dc.subjectSecreted Aspartic Proteinaseen_US
dc.subjectSterol 14alpha Demethylaseen_US
dc.subjectFluconazoleen_US
dc.subjectProteinaseen_US
dc.subjectSecreted Aspartic Protease 2en_US
dc.subjectSecreted Aspartic Proteinaseen_US
dc.subjectSterol 14alpha Demethylaseen_US
dc.subjectUnclassified Drugen_US
dc.subjectAdulten_US
dc.subjectAntifungal Resistanceen_US
dc.subjectAntifungal Susceptibilityen_US
dc.subjectArticleen_US
dc.subjectBroth Dilutionen_US
dc.subjectCandida Albicansen_US
dc.subjectControlled Studyen_US
dc.subjectEnzyme Activityen_US
dc.subjectFemaleen_US
dc.subjectFungal Strainen_US
dc.subjectFungus Isolationen_US
dc.subjectGene Expression Levelen_US
dc.subjectHumanen_US
dc.subjectMaleen_US
dc.subjectMinimum Inhibitory Concentrationen_US
dc.subjectNonhumanen_US
dc.subjectReal Time Polymerase Chain Reactionen_US
dc.subjectReal Time Reverse Transcription Polymerase Chain Reactionen_US
dc.subjectStrain Identificationen_US
dc.titleInvestigation of the relationship between fluconazole susceptibility, proteinase activity and ERG11-SAP2 Expression in Candida albicans strains isolated from clinical samplesen_US
dc.typeArticleen_US

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