The effects of ATP sensitive potassium channel (KATP) opener and blockers on Bcl-2, Bax, and Cyt-c gene expression levels in epileptic rats

Basit Öğe Kaydı
dc.authorscopusid57967943200
dc.authorscopusid57219390008
dc.contributor.authorKılıç, Ümit
dc.contributor.authorSoytürk, H.
dc.date.accessioned2023-07-26T11:58:59Z
dc.date.available2023-07-26T11:58:59Z
dc.date.issued2022
dc.departmentDÜ, Sağlık Hizmetleri Meslek Yüksekokulu, Tıbbi Hizmetler ve Teknikler Bölümüen_US
dc.description.abstractIn this study, it was aimed to investigate the gene expression levels of Bcl-2, Bax, and cytochrome c (Cyt-c), in the cortex region of pinacidil as a KATP channel opener and glibenclamide as a blocker on penicillin model epilepsy. Male Wistar-Albino rats were used. A total of 4 main groups were formed: Control, Epilepsy, Epilepsy-opener, and Epilepsy-blocker groups, then three-time points were formed subgroups (1st day, 4th, and 8th). 48 rats were used in total. The epileptic focus was created by intracortical administration of penicillin at a dose of 500 IU/2 ?l. Cortex is removed from all animals and cyt c, Bcl-2, and Bax gene expression levels were determined by qPCR. The SPSS 21 program was used for statistics. Bcl-2 and Bax gene expression levels were increased in the cortex regions of rats with epilepsy (p<0.05). Bcl-2, Bax gene expression levels, which increased due to epilepsy with the effect of KATP channels opened with pinacidil, returned to normal levels in the epilepsy opener group (p<0.05). Bcl-2 gene expression level, which was increased as a result of epilepsy due to the effect of KATP channels closed with glibenclamide, was higher than in the control and epilepsy-opener groups (p<0.05). Bcl-2 and Bax gene expression levels are increased in the cortex region due to epilepsy indicates that the apoptotic pathway could be activated. This study also It has been shown that the apoptotic pathway activated by epilepsy can be inactivated by pinacidil. © 2022 Ondokuz Mayis Universitesi. All rights reserved.en_US
dc.description.sponsorship2019.10.01.1408en_US
dc.description.sponsorshipThis study was financed by the Abant İzzet Baysal University Research Foundation (BAP-grant number: 2019.10.01.1408).en_US
dc.identifier.doi10.52142/omujecm.39.3.50
dc.identifier.endpage862en_US
dc.identifier.issn1309-4483
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-85142102884en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage857en_US
dc.identifier.urihttps://doi.org/10.52142/omujecm.39.3.50
dc.identifier.urihttps://hdl.handle.net/20.500.12684/13610
dc.identifier.volume39en_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorKılıç, Ümit
dc.language.isoenen_US
dc.publisherOndokuz Mayis Universitesien_US
dc.relation.ispartofJournal of Experimental and Clinical Medicine (Turkey)en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmz$2023V1Guncelleme$en_US
dc.subjectBaxen_US
dc.subjectBcl-2en_US
dc.subjectCytochrome cen_US
dc.subjectepilepsyen_US
dc.subjectKATP channelsen_US
dc.subjectadenosine triphosphate sensitive potassium channelen_US
dc.subjectcytochrome cen_US
dc.subjectglibenclamideen_US
dc.subjectpenicillin derivativeen_US
dc.subjectpinacidilen_US
dc.subjectprotein Baxen_US
dc.subjectprotein bcl 2en_US
dc.subjectanimal experimenten_US
dc.subjectanimal modelen_US
dc.subjectanimal tissueen_US
dc.subjectArticleen_US
dc.subjectbrain cortexen_US
dc.subjectcontrolled studyen_US
dc.subjectepilepsyen_US
dc.subjectepileptic focusen_US
dc.subjectgene expressionen_US
dc.subjectgene expression levelen_US
dc.subjecthippocampusen_US
dc.subjecthousekeeping geneen_US
dc.subjectmaleen_US
dc.subjectmRNA expression levelen_US
dc.subjectnonhumanen_US
dc.subjectpost hoc analysisen_US
dc.subjectraten_US
dc.subjectreal time polymerase chain reactionen_US
dc.titleThe effects of ATP sensitive potassium channel (KATP) opener and blockers on Bcl-2, Bax, and Cyt-c gene expression levels in epileptic ratsen_US
dc.typeArticleen_US

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim
İsim:
13610.pdf
Boyut:
563.82 KB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin / Full Text
İndir

Koleksiyon

Scopus İndeksli Yayınlar Koleksiyonu