Sıçanlarda intravenöz uygulanan deli bal toksini grayanotoksin-III'ün doza bağımlı kardiyak etkileri
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Tarih
2017
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Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Deli bal zehirlenmelerin büyük bir kısmı ülkemizde görülmektedir. Deli bal içinde bulunan toksinlerin temel kaynağı Rhododendron türü bitkiler olup, bu bitkilerdeki toksinlerden en fazla grayanotoksin-III bulunur. Deli bal zehirlenmelerinde klinikte bradikardi, atriyoventriküler blok, asistoli, hipotansiyon, salivasyon gibi semptomlar görülür. Bu çalışmada, sıçanlara farklı dozlarda intravenöz (i.v.) yolla verilen grayanotoksinIII'ün doza bağımlı kardiyovasküler etkilerinin değerlendirilmesi amaçlanmıştır.Yöntem: Çalışmada, 42 Wistar albino sıçan kullanılmış ve hayvanlar Kontrol, Grup 1 (5 µg/kg), Grup 2 (10 µg/kg), Grup 3 (20 µg/kg), Grup 4 (50 µg/kg) ve Grup 5 (100 µg/kg) olmak üzere altı gruba ayrılmıştır. Elektrokardiyogram (EKG), tansiyon arteriyel ve solunum sayısı için bazal değerler elde edildikten sonra i.v. grayanotoksin uygulanmasının ardından 60 dk. boyunca bu parametreler kaydedilmiştir.Bulgular: 5 µg/kg grayanotoksin-III verilen grupta, tüm zamanlarda anlamlı bir değişiklik olmazken, 10 ve 20 µg/kg toksin verilen gruplarda 2. ve 5. dk.'dan sonra anlamlı biçimde solunum sayıları ve kalp tepe atım sayıları azalmış, arteryel basınçlarda düşme görülmüştür. Yirmi beşinci dk.'dan sonra ise tüm parametreler bazal değerlere yeniden geri dönmüştür. Elli ve 100 µg/kg grayanotoksin-III verilen gruplarda, 2. dk.'dan itibaren tüm parametrelerde hızla düşme olmuş ve 5-10 dk. içinde sıçanlar ölmüştür. Sonuç: Sıçanlar için i.v. grayanotoksin-III'ün 50 µg/kg ve üzerindeki yüksek dozlarda lethal olabildiği, ölümün kardiyak arrest nedeniyle görüldüğü, 5 µg/kg ve altındaki düşük dozlarda toksik etkilerin görülmediği, i.v. uygulamada toksik etkilerin yaklaşık olarak 25 dk. sürdüğü saptanmıştır.
Objective: The mad honey poisonings are commonly seen in our country. The source of the toxin in mad honey is plants of Rhododendron species. Grayanotoxin-III type toxin is commonly found in these plants. Clinical effects of this toxin are bradycardia, atrioventricular block, asystole, hypotension and symptoms such as salivation. In this study; different doses of grayanotoxin-III were intravenously administered to rats (i.v) and the effect of grayanotoxin on cardiovascular system was evaluated.Methods: This study was performed on 42 Wistar albino rats and the animals were divided into six groups. Control, Group 1 (5 µg/kg), Group 2 (10 µg/kg), Group 3 (20 µg/kg), Group 4 (50 µg/kg) and Group 5 (100 µg/kg) groups were formed. Baseline values for electrocardiography (ECG), arterial blood pressure and respiratory rate were obtained and recorded for 60 minutes after administration of i.v. grayanotoxin-III.Results: Group1 (5 µg/kg) was not significantly changed in all time periods. In 10 and 20 µg/kg groups, respiratory rates, peak heart rates decreased significantly and drop in arterial blood pressures was observed after 2nd and 5th minutes. While 25 minutes after administration of i.v. grayanotoxin-III all parameters returned to baseline values again. In 50 and 100 µg/kg grayanotoxin-III groups all parameters fell sharply from 2 minutes on, and rats died within 5-10 minutes.Conclusion: In conclusion, it was found that the lethal dose of grayanotoxin-III for Kabul tarihi: 07.06.2017 rats is 50 µg/kg. In addition the death was developed due to cardiac arrest. In case of the lower level of 5 µg/kg grayanotoxin-III, any toxic effect was not seen. The effects of toxin apparently lasted nearly 25 minutes.
Objective: The mad honey poisonings are commonly seen in our country. The source of the toxin in mad honey is plants of Rhododendron species. Grayanotoxin-III type toxin is commonly found in these plants. Clinical effects of this toxin are bradycardia, atrioventricular block, asystole, hypotension and symptoms such as salivation. In this study; different doses of grayanotoxin-III were intravenously administered to rats (i.v) and the effect of grayanotoxin on cardiovascular system was evaluated.Methods: This study was performed on 42 Wistar albino rats and the animals were divided into six groups. Control, Group 1 (5 µg/kg), Group 2 (10 µg/kg), Group 3 (20 µg/kg), Group 4 (50 µg/kg) and Group 5 (100 µg/kg) groups were formed. Baseline values for electrocardiography (ECG), arterial blood pressure and respiratory rate were obtained and recorded for 60 minutes after administration of i.v. grayanotoxin-III.Results: Group1 (5 µg/kg) was not significantly changed in all time periods. In 10 and 20 µg/kg groups, respiratory rates, peak heart rates decreased significantly and drop in arterial blood pressures was observed after 2nd and 5th minutes. While 25 minutes after administration of i.v. grayanotoxin-III all parameters returned to baseline values again. In 50 and 100 µg/kg grayanotoxin-III groups all parameters fell sharply from 2 minutes on, and rats died within 5-10 minutes.Conclusion: In conclusion, it was found that the lethal dose of grayanotoxin-III for Kabul tarihi: 07.06.2017 rats is 50 µg/kg. In addition the death was developed due to cardiac arrest. In case of the lower level of 5 µg/kg grayanotoxin-III, any toxic effect was not seen. The effects of toxin apparently lasted nearly 25 minutes.
Açıklama
Anahtar Kelimeler
Pediatri
Kaynak
İzmir Dr. Behçet Uz Çocuk Hastanesi Dergisi
WoS Q Değeri
Scopus Q Değeri
Cilt
7
Sayı
2
