Lamium galeobdolon (L.) L. ve Lamium purpureum L. Etanolik Ekstraktlarının Antimikrobiyal, Anti-quorum sensing ve Antibiyofilm Potansiyelleri
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Date
2024
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Oktay YILDIZ
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info:eu-repo/semantics/openAccess
Abstract
Bakteriler, patojeniteyi kontrol etmek için quorum sensing (QS) adı verilen bir iletişim mekanizması yoluyla hareket eder. Biyofilm oluşumu QS mekanizması tarafından desteklenen bir süreçtir ve antibiyotik direncinde rol oynadığı bilinmektedir. Çalışmada Lamium galeobdolon ve Lamium purpureum bitkilerinden elde edilen etanol ekstraktlarının antimikrobiyal, anti-QS ve antibiyofilm kapasiteleri belirlendi. Bitki ekstraktlarının antimikrobiyal aktivitesi çeşitli hastane izolatlarına karşı kuyu difüzyon deneyi ile araştırılmıştır. Ekstraktlar, tüm bakteri ve mayalara karşı antimikrobiyal etki göstermiştir. L. purpureum'un 100 mg/mL dozunda Candida guilliermondii mayasına karşı 27.5 ± 0.71 mm'lik inhibitör etkisi dikkat çekicidir. Ekstraktların anti-QS potansiyeli, viyolasin pigment inhibisyonuna dayanan kuyu difüzyon deneyi ile değerlendirildi. L. purpureum ekstraktı, Chromobacterium violaceum ATCC 12472 biyosensör suşuna karşı daha yüksek seviyede anti-QS etkisi gösterdi. Biyofilm oluşturan Escherichia coli izolatına karşı antibiyofilm kapasitesi kristal viyole boyama yöntemiyle belirlendi. L. purpureum ekstraktı en yüksek dozda E. colibiyofilmine karşı %89.66 oranında inhibitör etki göstermiştir. Her iki bitkinin de anti-patojenik özelliklere sahip olduğu kanıtlanmıştır. Ancak L. purpureum bitkisinin, L. galeobdolon bitkisine göre anti-patojenik özelliği daha yüksek bir bitki olduğu söylenebilir.
Bacteria act through a communication mechanism called quorum sensing (QS) to control pathogenicity. Biofilm formation is a process supported by the QS mechanism and is known to act a part in antibiotic resistance. In this study, antimicrobial, anti-QS and antibiofilm capacities of ethanol extracts obtained from Lamium galeobdolon (L.) L. and Lamium purpureum L. plants were determined. The antimicrobial activity of plant extracts was evaluated by well diffusion assay against various hospital isolates. The extracts have antimicrobial effects against all bacteria and yeasts. The 27.5 ± 0.71 mm inhibitory effect of L. purpureum against Candida guilliermondii yeast at a dose of 100 mg/mL is remarkable. The anti-QS potential of the extracts was evaluated by a well diffusion assay based upon violacein pigment inhibition. L. purpureum extract showed a higher level of anti-QS effect against Chromobacterium violaceum ATCC 12472 biosensor strain. Antibiofilm capacity against biofilm-forming Escherichia coli isolate was determined by the crystal violet staining method. L. purpureum extract showed an inhibitory effect of 89.66% against E.coli biofilm at the highest dose. Both plants have been shown to have anti-pathogenic properties. However, it can be said that the L.purpureum plant is a more highly anti-pathogenic plant compared to L. galeobdolon.
Bacteria act through a communication mechanism called quorum sensing (QS) to control pathogenicity. Biofilm formation is a process supported by the QS mechanism and is known to act a part in antibiotic resistance. In this study, antimicrobial, anti-QS and antibiofilm capacities of ethanol extracts obtained from Lamium galeobdolon (L.) L. and Lamium purpureum L. plants were determined. The antimicrobial activity of plant extracts was evaluated by well diffusion assay against various hospital isolates. The extracts have antimicrobial effects against all bacteria and yeasts. The 27.5 ± 0.71 mm inhibitory effect of L. purpureum against Candida guilliermondii yeast at a dose of 100 mg/mL is remarkable. The anti-QS potential of the extracts was evaluated by a well diffusion assay based upon violacein pigment inhibition. L. purpureum extract showed a higher level of anti-QS effect against Chromobacterium violaceum ATCC 12472 biosensor strain. Antibiofilm capacity against biofilm-forming Escherichia coli isolate was determined by the crystal violet staining method. L. purpureum extract showed an inhibitory effect of 89.66% against E.coli biofilm at the highest dose. Both plants have been shown to have anti-pathogenic properties. However, it can be said that the L.purpureum plant is a more highly anti-pathogenic plant compared to L. galeobdolon.
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Keywords
Antibiyofilm|Antimikrobiyal|Chromobacterium violaceum ATCC 12472|Lamium galeobdolon|Lamium purpureum|Antibiofilm|Antimicrobial|Chromobacterium violaceum ATCC 12472|Lamium galeobdolon|Lamium purpureum
Journal or Series
Journal of Apitherapy and Nature
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Scopus Q Value
Volume
7
Issue
1
