Dokosahekzaenoik asit' in (OMEGA-3 çoklu doymamış yağ asidi) ve krill yağı' nın rat modellerinde over rezervi üzerine etkisi
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Dosyalar
Tarih
2019
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Düzce Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Dokosahekzaenoik Asit' in (Omega-3 Çoklu Doymamış Yağ Asidi) ve Krill Yağının Rat Modellerinde Over Rezervi Üzerine Etkisi İnfertilite kontraseptif yöntem kullanmaksızın 1 yıl düzenli ilişkiyle gebelik elde edilememesidir. Reprodüktif çağdaki çiftlerin %15'ini etkilemektedir. İnflamasyon ve oksidatif stres ovulasyon ve implantasyon olaylarında rol alır. Antioksidan sistemin çalışmasında Omega-3 etkin rol oynamaktadır. Krill yağında Omega-3 yağ asitleri, fosfolipitler, kolin, astaksantin ve güçlü antioksidanlar bulunmaktadır. Bu çalışmanın amacı ratlarda Omega-3 ve Krill yağı desteğinin antral folikül sayısı, atreziye giden folikül yüzdesi ve serum AMH düzeyindeki değişim parametreleri eşliğinde overyan rezerve etkisini incelemektir. Bu çalışma 24adet 100±20gram Sprague-Dawley cinsi dişi ratlarda 4 östrus siklusu boyunca gerçekleştirilmiştir. 1.gruba ek hiçbir destek ürünü verilmemiştir. 2.gruba mide gavajıyla Omega-3-LCPUFA(450mg/kg/gün), 3.gruba Krill yağı(500mg/kg/gün) verilmiştir. Çalışmanın başlangıcı ve bitişinde alınan serum örneklerinden AMH düzeyi incelenmiştir. İmmünohistokimyasal analizde primordiyal, primer, preantral, antral ve atrezik folikül ve korpus luteum sayıları sayılmıştır. Çalışma çift kör yöntemiyle, İstatistiksel analizde SPSS for Windows 22.00 istatistik paket programı kullanılarak yapılmış, p<0,05 önem düzeyinde anlamlılık olarak kabul edilmiştir. Kontrol grubundaki primordiyal folikül sayısının Omega-3 grubundan(p=0,034); Krill yağı grubundaki primer folikül sayısının Omega-3 grubundan(p=0,035) daha fazla olduğu saptanmıştır. Gruplar arasında preantral, antral folikül ve toplam folikül sayılarında fark olmadığı görülmüştür. Kontrol grubundaki korpus luteum sayısının Omega-3 grubundan daha fazla olduğu saptanmıştır(p=0,044). Atrezik folikül yüzdesi en yüksek Omega-3 grubunda görülmüştür(p=0,00). Tüm gruplarda deney öncesi/sonrası AMH değerlerindeki değişim istatistiksel olarak anlamsız bulunmuştur(p=0,161).Çalışmada ratlarda Krill yağı ve Omega-3 yağ asidinin folikül rezervini histolojik olarak ve gerekse serum parametreleri açısından olumlu yönde etkilemediği gösterilmiştir. İnfertilitenin en önemli nedenlerinden olan düşük over rezervinin tedavisinde rutin olarak güvenle verilebilecek ajanların keşfedilmesi için kapsamlı ve randomize yeni çalışmalara gereksinim vardır. Anahtar Sözcükler: İnfertilite, over rezervi, Omega-3, Krill yağı, AMH (anti-Müllerian Hormaon), folikül, atrezi.
The Effect of Omega-3 Polyunsaturated Fatty Acid and Krill Oil on The Ovarian Reserve in Rat Model Infertility is the inability to get pregnant with regular intercourse for 1 year without using a contraceptive method. It affects 15% of the couples in reproductive age. Inflammation and oxidative stress are involved in ovulation and implantation. Omega-3 plays an active role in the antioxidant system. Krill oil contains Omega-3 fatty acids, phospholipids, choline, astaxanthin and potent antioxidants.The aim of this study was to investigate the effects of Omega-3 and Krill oil supplementation on antral follicle count in regards to the number of follicles undergoing atresia and the change in serum AMH levels.This study was done with 24 female Sprague-Dawley female rats 100±20 grams during 4 oestrus cycles. No additional supplemantation products were given to Group 1. The second and the third groups were given Omega-3-LCPUFA (450mg/kg/day) and Krill oil (500mg/kg/day) by gastric gavage respectively. Serum AMH levels were examined at the beginning and end of the study, In the immunohistochemical analysis, primordial, primary, preantral, antral and atresic follicles and corpus luteums were counted. The study was carried out by using double blind method, statistical package program.SPSS for Windows 22.00, p <0.05 was considered as the significance level.Number of primordial follicles in the control group was higher compared to the Omega-3 group(p=0.034); and primary follicle count in the Krill oil group was higher than the Omega-3 group(p=0.035). There was no difference between the groups in terms of preantral, antral follicle and total follicle numbers. Number of corpus luteums in the control group was higher than the Omega-3 group(p=0.044). The highest atresic follicle percentages were in the Omega-3 group(p=0.00). The change in AMH values before and after the study was statistically insignificant in all groups(p=0,161).In this study, Krill oil and Omega-3 fatty acid supplemantation in rats were not shown to affect the follicle reserve positively in terms of histological and serum parameters. In order to discover the agents that can be safely administered in the treatment of low ovarian reserve which is one of the most important causes of infertility, new comprehensive and randomized studies are needed.
The Effect of Omega-3 Polyunsaturated Fatty Acid and Krill Oil on The Ovarian Reserve in Rat Model Infertility is the inability to get pregnant with regular intercourse for 1 year without using a contraceptive method. It affects 15% of the couples in reproductive age. Inflammation and oxidative stress are involved in ovulation and implantation. Omega-3 plays an active role in the antioxidant system. Krill oil contains Omega-3 fatty acids, phospholipids, choline, astaxanthin and potent antioxidants.The aim of this study was to investigate the effects of Omega-3 and Krill oil supplementation on antral follicle count in regards to the number of follicles undergoing atresia and the change in serum AMH levels.This study was done with 24 female Sprague-Dawley female rats 100±20 grams during 4 oestrus cycles. No additional supplemantation products were given to Group 1. The second and the third groups were given Omega-3-LCPUFA (450mg/kg/day) and Krill oil (500mg/kg/day) by gastric gavage respectively. Serum AMH levels were examined at the beginning and end of the study, In the immunohistochemical analysis, primordial, primary, preantral, antral and atresic follicles and corpus luteums were counted. The study was carried out by using double blind method, statistical package program.SPSS for Windows 22.00, p <0.05 was considered as the significance level.Number of primordial follicles in the control group was higher compared to the Omega-3 group(p=0.034); and primary follicle count in the Krill oil group was higher than the Omega-3 group(p=0.035). There was no difference between the groups in terms of preantral, antral follicle and total follicle numbers. Number of corpus luteums in the control group was higher than the Omega-3 group(p=0.044). The highest atresic follicle percentages were in the Omega-3 group(p=0.00). The change in AMH values before and after the study was statistically insignificant in all groups(p=0,161).In this study, Krill oil and Omega-3 fatty acid supplemantation in rats were not shown to affect the follicle reserve positively in terms of histological and serum parameters. In order to discover the agents that can be safely administered in the treatment of low ovarian reserve which is one of the most important causes of infertility, new comprehensive and randomized studies are needed.
Açıklama
YÖK Tez No: 538657
Anahtar Kelimeler
Kadın Hastalıkları ve Doğum, Obstetrics and Gynecology, Anti-müllerian hormon, Anti-mullerian hormone, Dokosahekzaenoik asitler, Docosahexaenoic acids, Foliküler atrezi, Follicular atresia, Hayvan deneyleri, Animal experimentation, Kril, Krill, Kısırlık, Infertility, Over, Ovary, Sıçanlar, Rats, Yağ asitleri-doymamış, Fatty acids-unsaturated, Yağ asitleri-omega 3, Fatty acids-omega 3, İnfertilty, ovarian reserve, Omega-3, Krill oil, AMH (Anti-Müllerian Hormone), follicle, atresia
