The effect of magnetic field strength on shoot regeneration and Agrobacterium tumefaciens-mediated gene transfer in flax (Linum usitatissimum L.)

Basit Öğe Kaydı
dc.contributor.authorAycan, Murat
dc.contributor.authorBeyaz, Ramazan
dc.contributor.authorBahadır, Anzel
dc.contributor.authorYıldız, Mustafa
dc.date.accessioned2020-04-30T23:33:01Z
dc.date.available2020-04-30T23:33:01Z
dc.date.issued2019
dc.departmentDÜ, Tıp Fakültesi, Dahili Tıp Bilimleri Bölümüen_US
dc.descriptionWOS: 000460753400004en_US
dc.description.abstractThis study was conducted to determine the effects of magnetic field (MF) strength on shoot regeneration and Agrobacterium tumefaciens-mediated gene transfer in flax (Linum usitatissimum L.). Seeds of flax cv. Madaras were exposed to different MF strengths (0 - control, 75, 150, and 300 millitesla (mT)) for 24 h by using an electromagnetic generator system fabricated in laboratory conditions. After sterilization, seeds were germinated on MS (Murashige and Skoog) medium in Magenta vessels. Hypocotyl explants excised from 7-days-old seedlings were used for regeneration. GV2260 strain of Agrobacterium tumefaciens was used in transformation studies. Inoculated hypocotyls were cultured on MS medium containing 1 mg/l BAP (6-benzylaminopurine) and 0.02 mg/l NAA (naphthaleneacetic acid) for 2 days by co-cultivation. Then, they were transferred to MS medium containing the same growth regulators, 100 mg/l kanamycin and 500 mg/l Duocid for selection. The presence of the nptII gene was verified by PCR (polymerase chain reaction) analysis in putative transgenic plants. The highest results with respect to shoot regeneration and transformation frequency were obtained from treatments of 75 mT MF strength.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [113O280]en_US
dc.description.sponsorshipThis investigation was supported by Scientific and Technological Research Council of Turkey (TUBITAK); Grant No. 113O280 to Prof. Dr. M. YILDIZen_US
dc.identifier.doi10.17221/195/2017-CJGPBen_US
dc.identifier.endpage27en_US
dc.identifier.issn1212-1975
dc.identifier.issn1805-9325
dc.identifier.issue1en_US
dc.identifier.startpage20en_US
dc.identifier.urihttps://doi.org/10.17221/195/2017-CJGPB
dc.identifier.urihttps://hdl.handle.net/20.500.12684/4876
dc.identifier.volume55en_US
dc.identifier.wosWOS:000460753400004en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherCzech Academy Agricultural Sciencesen_US
dc.relation.ispartofCzech Journal Of Genetics And Plant Breedingen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAgrobacterium tumefaciensen_US
dc.subjectLinum usitatissimumen_US
dc.subjectmagnetic fielden_US
dc.subjectregeneration efficiencyen_US
dc.subjecttransformationen_US
dc.titleThe effect of magnetic field strength on shoot regeneration and Agrobacterium tumefaciens-mediated gene transfer in flax (Linum usitatissimum L.)en_US
dc.typeArticleen_US

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim
İsim:
4876.pdf
Boyut:
843.06 KB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin / Full Text
İndir

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Makale Koleksiyonu