NF-?B and MAPKs are involved in resistin-caused ADAMTS-5 induction in human chondrocytes

Basit Öğe Kaydı
dc.contributor.authorHatipoğlu, Ömer Faruk
dc.contributor.authorYaykaşlı, Kürşat Oğuz
dc.contributor.authorDoğan, Mustafa
dc.contributor.authorYaykaşlı, Emine
dc.contributor.authorBender, Onur
dc.contributor.authorYaşar, Tuğçe
dc.contributor.authorGündüz, Mehmet
dc.date.accessioned2020-04-30T13:32:52Z
dc.date.available2020-04-30T13:32:52Z
dc.date.issued2015
dc.departmentDÜ, Tıp Fakültesi, Dahili Tıp Bilimleri Bölümüen_US
dc.description.abstractPurpose: Chronic inflammation is an important etiological factor in the development of arthritic diseases. Several factors contribute to aggregation of chronic inflammation, including the presence of excess adipose tissue. Methods: The putative induction mechanisms of ADAMTS-5 by resistin were investigated in normal primary human articular chondrocytes. Expression levels of the ADAMTS-5 gene were determined at several resistin doses and durations. Results: Human chondrocytes were activated and associated with upregulated ADAMTS-5 gene expression after exposure to resistin (also known as adipose tissue-specific secretary factor, ADSF). Release of ADAMTS-5 leads to joint cartilage degradation, a key event in the development of arthritic diseases rheumatoid arthritis (RA) and osteoarthritis (OA). Activation of chondrocytes was associated with upregulated NF-?B protein levels in a time-dependent fashion. Co-incubation of human chondrocytes with JNK and p38 inhibitors lead to abrogated levels of NF-?B, indicating that these MAPKs are important in the activation of chondrocytes after stimulation with resistin. Similarly, ADAMTS-5 expression levels were abrogated when co-incubated with p38, NF-?B, JNK, MEK and PI3K inhibitors. Our results demonstrate that resistin, released from adipose tissue, may be involved in the development of RA and OA in obese patients through degradation of joint cartilage via ADAMTS-5 released from activated chondrocytes. © 2015 CIM.en_US
dc.identifier.endpageE254en_US
dc.identifier.issn0147958X
dc.identifier.issue4en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpageE248en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12684/467
dc.identifier.volume38en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherThe Canadian Society for Clinical Investigationen_US
dc.relation.ispartofClinical and Investigative Medicineen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.titleNF-?B and MAPKs are involved in resistin-caused ADAMTS-5 induction in human chondrocytesen_US
dc.typeArticleen_US

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim Yok
İsim:
0467.pdf
Boyut:
891.42 KB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin / Full Text
İndir

Koleksiyon

Scopus İndeksli Yayınlar Koleksiyonu
Makale Koleksiyonu