Lupeol'un erkek sıçanlarda akut egzersize bağlı oksidatif stres üzerine koruyucu etkileri
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Date
2023
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Düzce Üniversitesi
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info:eu-repo/semantics/openAccess
Abstract
Akut egzersiz, özellikle yüksek şiddette yapıldığı zaman oksijen tüketimini artırması sebebiyle serbest radikal üretiminde artışa yol açmaktadır. Lupan tip triterpen yapısındaki, Lupeol antioksidan antikanser ve antiinflamatuvar etkilerinin yanısıra, serbest radikalleri inhibe edici özelliği ile bilinmektedir. Bu çalışmanın amacı, lupeol'un erkek sıçanlarda akut egzersize bağlı oksidatif stres üzerine koruyucu etkilerinin belirlenmesidir. Araştırmamızda 6 aylık, 200±250 g. ağırlığında 24 adet Spraque Dawley cinsi sıçanlar kullanılmıştır. Deney hayvanları rastgele yöntem ile seçilerek kontrol, egzersiz, lupeol ve egzersiz + lupeol olarak dört gruba ayrılmıştır. Araştırma sonrası deney hayvanları sakrifiye (servikal dislokasyon) edildikten sonra alınan kan örneklerinde; enzimatik antioksidanlar, (SOD),(CAT), (MDA); nonenzimatik grup, (GPx), koenzim Q10 ve a-lipoik asit enzimlerinin düzeyleri ve serum örneklerindeki oksidatif stres düzeyleri ELISA yöntemiyle ölçülmüştür. Analiz sonuçları istatistik programı ile grupların tüm analizleri yapılmış anlamlılık düzeyi p<0,05 olarak kabul edilmiştir. Sonuç olarak; Lupeol'un vücudun üretmiş olduğu antioksidan seviyesine olumlu yönde katkıda bulunduğu ve oksidanları baskılamada yardımcı olduğu tespit edilmiş olup, yüksek şiddetli egzersiz sonucu ortaya çıkan oksidatif stres kaynaklı serbest radikallerin lupeol tarafından azaltıldığı gözlemlenmiştir. Buna bağlı olarak oksidatif strese maruz kalan deney hayvanlarında egzersizde lupeol kullanımı, stresten oluşan serbest radikalleri inhibe ettiği, çalışmanın hipotezinin destekler nitelikte olduğu tespit edilmiştir. Anahtar Sözcükler: Lupeol, Sıçan, Egzersiz, Oksidatif Stres, Serbest Radikal
Acute exercise, especially when done at high intensity, leads to an increase in free radical production due to increased oxygen consumption. Lupeol is known for its antioxidant and sterol properties, as well as its free radical inhibitory properties in anticancer and anti-inflammatory conditions. The aim of our study was to determine the protective effects of lupeol on acute exercise-induced oxidative stress in male rats. In our study, 6 months old, 200±250 g. Weight of 24 spraque dawley rats were used. Experimental animals were randomly selected and divided into four groups. Blood samples taken after the animals were sacrificed (cervical dislocation) after acute exercise; enzymatic antioxidants, (SOD), (CAT), (MDA); nonenzymatic group, (GPx), coenzyme Q10 and a-lipoic acid enzymes were examined and oxidative stress levels were measured from the serum of blood samples taken by ELISA method. All analyzes of the groups were made with the statistical program of the analysis results, and the significance level was accepted as p<0.05. As a result; It has been determined that lupeol contributes positively to the antioxidant level produced by the body and helps in suppressing oxidants, and it has been observed that free radicals caused by oxidative stress, which occur as a result of high-intensity exercise, are reduced by lupeol. Accordingly, it was determined that the use of lupeol in exercise in experimental animals exposed to oxidative stress inhibited free radicals caused by stress, which supports the hypothesis of the study. Keywords: Lupeol, Rat, Exercise, Oxidative Stress, Free Radical
Acute exercise, especially when done at high intensity, leads to an increase in free radical production due to increased oxygen consumption. Lupeol is known for its antioxidant and sterol properties, as well as its free radical inhibitory properties in anticancer and anti-inflammatory conditions. The aim of our study was to determine the protective effects of lupeol on acute exercise-induced oxidative stress in male rats. In our study, 6 months old, 200±250 g. Weight of 24 spraque dawley rats were used. Experimental animals were randomly selected and divided into four groups. Blood samples taken after the animals were sacrificed (cervical dislocation) after acute exercise; enzymatic antioxidants, (SOD), (CAT), (MDA); nonenzymatic group, (GPx), coenzyme Q10 and a-lipoic acid enzymes were examined and oxidative stress levels were measured from the serum of blood samples taken by ELISA method. All analyzes of the groups were made with the statistical program of the analysis results, and the significance level was accepted as p<0.05. As a result; It has been determined that lupeol contributes positively to the antioxidant level produced by the body and helps in suppressing oxidants, and it has been observed that free radicals caused by oxidative stress, which occur as a result of high-intensity exercise, are reduced by lupeol. Accordingly, it was determined that the use of lupeol in exercise in experimental animals exposed to oxidative stress inhibited free radicals caused by stress, which supports the hypothesis of the study. Keywords: Lupeol, Rat, Exercise, Oxidative Stress, Free Radical
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Sağlık Eğitimi, Health Education, Spor
