İndirekt immünofloresan yöntemi ile değerlendirilen otoantikor pozitifliklerine SARS-COV-2 pandemi etkisinin araştırılması
Küçük Resim Yok
Tarih
2023
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Düzce Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Çalışmamızın amacı, SARS-CoV-2 enfeksiyonu ile sistemik ve organ spesifik otoimmün hastalıklar arasındaki olası ilişkinin belirlenmesidir. Gereç ve Yöntem: Çalışmamıza, Mart 2018-Mart 2022 tarihleri arasında Düzce Üniversitesi Tıp Fakültesi, Tıbbi Mikrobiyoloji Laboratuvarı'na çeşitli kliniklerdeki hastalardan otoimmün hastalık şüphesi ile gönderilen serum örnekleri dahil edildi. İndirekt immunofloresan antikor parametreleri ve aynı hastalardan bu süreçte laboratuvarımızda COVID-19 PCR testi çalışılan örneklerin sonuçları, hastanemizin otomasyon sisteminden elde edilerek retrospektif olarak değerlendirildi. Bulgular ve sonuç: Çalışmaya, COVID-19 pandemi öncesi iki yıl ve pandemi dönemindeki iki yılda otoimmun hastalık şüphesiyle IIFA testi için mikrobiyoloji laboratuvarına serum örnekleri gönderilen 8325 hastadan herhangi bir otoantikoru pozitif saptanan 2448 hasta dahil edildi. ANA pozitifliği, toplam 2256 hastada saptanmış olup bunlardan 2038'inin tekli (1363'ü benekli patern), 218'inin çoklu patern olduğu görüldü. ANA testi çalışılmış ve COVID-19 PZR pozitifliği saptanmış olan hastaların 41'inde ANA pozitifliği tespit edilmiş olup patern olarak en sık benekli (%74) patern saptandı. Otoantikor pozitifliğinin zamana göre değişimini incelendiğinde ANA, anti-dsDNA, anti-ICA, anti-Gliadin otoantikorlarının pozitiflik oranlarında pandemi dönemini içeren iki yıllık sürede istatistiksel olarak anlamlı artışın olduğu tespit edildi. AMA, ASMA, anti-Endomisyum, ANCA otoantikorlarının pozitiflik oranlarında ise COVID-19 pandemi öncesi ve pandemisini içeren tüm dönemlerde fark olmadığı görüldü. Otoantikor pozitifliğine sahip hastaların, hastanemiz Mikrobiyoloji Laboratuvarı'nda çalışılmış COVID-19 PZR sonuçları incelendiğinde 323 hastanın COVID-19 PCR testinin pozitif, 306 hastanın ise negatif olduğu görüldü. ANAHTAR SÖZCÜKLER: İndirekt immunofloresan antikor yöntemi, otoantikor, otoimmün hastalıklar, SARS-CoV-2, COVID-19
Background: The aim of our study is to determine the possible relationship between SARS-CoV-2 infection and systemic and organ-specific autoimmune diseases. Material and Method: Serum samples sent to Düzce University Faculty of Medicine, Medical Microbiology Laboratory from patients in various clinics with suspicion of autoimmune disease between March 2018 and March 2022 were included in our study. Indirect immunofluorescence antibody parameters and the results of samples from the same patients, for which COVID-19 PCR tests were performed in our laboratory during this period, were obtained from the automation system of our hospital and evaluated retrospectively. Results: Of the 8325 patients whose serum samples were sent to the microbiology laboratory for IIFA testing with suspicion of autoimmune disease in the two years before the COVID-19 pandemic and in the two years during the pandemic period, 2448 patients who were detected positive for any autoantibody were included in the study. ANA positivity was detected in a total of 2256 patients, 2038 of whom had a single pattern (1363 had a spotted pattern) and 218 had a multiple pattern. ANA positivity was detected in 41 of the patients whose ANA test was studied and COVID-19 PCR positivity was detected, and the most common pattern was spotted (74%). When the change in autoantibody positivity over time was examined, it was determined that there was a statistically significant increase in the positivity rates of ANA, anti-dsDNA, anti-ICA, and anti-Gliadin autoantibodies in the two-year period, including the pandemic period. It was observed that there was no difference in the positivity rates of AMA, ASMA, anti-Endomysium, ANCA autoantibodies in all periods before and during the COVID-19 pandemic. When the COVID-19 PCR results of patients with autoantibody positivity, studied in the Microbiology Laboratory of our hospital, were examined, it was seen that the COVID-19 PCR test of 323 patients was positive and 306 patients were negative. KEYWORDS: Indirect immunofluorescent antibody method, autoantibody, autoimmune diseases, SARS-CoV-2, COVID-19
Background: The aim of our study is to determine the possible relationship between SARS-CoV-2 infection and systemic and organ-specific autoimmune diseases. Material and Method: Serum samples sent to Düzce University Faculty of Medicine, Medical Microbiology Laboratory from patients in various clinics with suspicion of autoimmune disease between March 2018 and March 2022 were included in our study. Indirect immunofluorescence antibody parameters and the results of samples from the same patients, for which COVID-19 PCR tests were performed in our laboratory during this period, were obtained from the automation system of our hospital and evaluated retrospectively. Results: Of the 8325 patients whose serum samples were sent to the microbiology laboratory for IIFA testing with suspicion of autoimmune disease in the two years before the COVID-19 pandemic and in the two years during the pandemic period, 2448 patients who were detected positive for any autoantibody were included in the study. ANA positivity was detected in a total of 2256 patients, 2038 of whom had a single pattern (1363 had a spotted pattern) and 218 had a multiple pattern. ANA positivity was detected in 41 of the patients whose ANA test was studied and COVID-19 PCR positivity was detected, and the most common pattern was spotted (74%). When the change in autoantibody positivity over time was examined, it was determined that there was a statistically significant increase in the positivity rates of ANA, anti-dsDNA, anti-ICA, and anti-Gliadin autoantibodies in the two-year period, including the pandemic period. It was observed that there was no difference in the positivity rates of AMA, ASMA, anti-Endomysium, ANCA autoantibodies in all periods before and during the COVID-19 pandemic. When the COVID-19 PCR results of patients with autoantibody positivity, studied in the Microbiology Laboratory of our hospital, were examined, it was seen that the COVID-19 PCR test of 323 patients was positive and 306 patients were negative. KEYWORDS: Indirect immunofluorescent antibody method, autoantibody, autoimmune diseases, SARS-CoV-2, COVID-19
Açıklama
Anahtar Kelimeler
Mikrobiyoloji, Microbiology
