May argyrophilic nucleolar organizer regions be used as a biomarker for the detection of the degree of ischemic damage instead of tunel in testicular torsion?

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dc.authorscopusid56671407500
dc.authorscopusid34868115100
dc.authorscopusid57198376648
dc.contributor.authorKabaklıoğlu, M.
dc.contributor.authorEroz, R.
dc.contributor.authorKaya, M.
dc.date.accessioned2021-12-01T18:38:52Z
dc.date.available2021-12-01T18:38:52Z
dc.date.issued2021
dc.department[Belirlenecek]en_US
dc.description.abstractBackground and Objectives: It is of great importance to obtain information about the severity of ischemic damage and duration of testicular torsion for an effective treatment strategy. Nucleolar-organizing regions (NORs) are sites of the ribosomal genes composed of ribosomal DNA and proteins. Post-silver staining NORs are termed “AgNOR”. Since AgNORs clearly reveals the self-renewal potential of cells damaged in ischemic events, we performed the current study. Materials and Methods: The study was carried out in four groups as control, sham, early, and late T/D. In the surgical groups, testes were corrected after a 4-h ischemia period. Testicular tissue samples were taken on the third day after detorsion in group 1, 2, 3, and on the tenth day after detorsion in group 4. TUNEL and silver stainings were applied to all samples. Results: The differences were significant among the groups for both mean AgNOR number and total AgNOR area/total nuclear area (TAA/TNA). Moreover, the differences between control and early torsion-detorsion (T/D), between control and late T/D, between sham and early T/D, between sham and late T/D, and between early T/D and late were statistically significant for AgNOR amount. Furthermore, statistically significant differences among the groups for an average number of apoptotic cells per tubule and the percentage of apoptotic tubule values were detected. Discussion: The apoptotic index gives the ratio of cells that are damaged and will die in a programmed way and cells that remain intact, rather than show the viability of the returning testicle. However, by measuring cells that regenerate with AgNOR, we can show not only those that survive but also cells that can repair themselves. Conclusion: AgNOR proteins are usable for the early observation of ischemic injury levels. The amount of AgNOR protein can enlighten us about the extent of testicular damage after T/D treatment. It may also help the physician in the development of effective treatment strategies for cases. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.en_US
dc.identifier.doi10.3390/medicina57111177
dc.identifier.issn1010660X
dc.identifier.issue11en_US
dc.identifier.pmid34833395en_US
dc.identifier.scopus2-s2.0-85118681907en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.3390/medicina57111177
dc.identifier.urihttps://hdl.handle.net/20.500.12684/9884
dc.identifier.volume57en_US
dc.identifier.wosWOS:000758106100001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.relation.ispartofMedicina (Lithuania)en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAgNORen_US
dc.subjectIschemiaen_US
dc.subjectNucleolar organizer regionsen_US
dc.subjectRDNAen_US
dc.subjectTestisen_US
dc.titleMay argyrophilic nucleolar organizer regions be used as a biomarker for the detection of the degree of ischemic damage instead of tunel in testicular torsion?en_US
dc.typeArticleen_US

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