Evaluation and comparison of alpha- and beta-amanitin toxicity on MCF-7 cell line

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dc.contributor.authorKaya, Ertuğrul
dc.contributor.authorBayram, Recep
dc.contributor.authorYaykaşlı, Kürşat Oğuz
dc.contributor.authorYılmaz, İsmail
dc.contributor.authorBayram, Sait
dc.contributor.authorYaykaşlı, Emine
dc.contributor.authorGepdiremen, Ali Akçahan
dc.date.accessioned2020-04-30T14:39:12Z
dc.date.available2020-04-30T14:39:12Z
dc.date.issued2014
dc.departmentDÜ, Tıp Fakültesi, Dahili Tıp Bilimleri Bölümüen_US
dc.description.abstractBackground/aim: Alpha- and beta-amanitins are the main toxins of the poisonous Amanita phalloides mushroom. Although there are many studies available concerning alpha-amanitin, there are limited data about beta-amanitin in the literature. Therefore, this study is aimed at comparing the toxic effects of alpha- and beta-amanitin on the MCF-7 cell line. Materials and methods: The alpha- and beta-amanitins used for this research were purified from Amanita phalloides by preparative high-performance liquid chromatography. The MCF-7 breast cancer cell line was used, and specific concentrations of the toxins (100, 10, 1, 0.1, and 0.01 µg/mL) were applied to the cells. The MTT test was performed to determine the level of toxicity, and the quantity of protein in the cell was measured using the biuret test. Results: The alpha-amanitin showed a higher toxicity at 36 h, while the highest inhibition of protein synthesis by the beta-amanitin was observed at 24 h. Conclusion: It was shown that the beta-amanitin may be responsible for toxicity, like alpha-amanitin, in Amanita phalloides mushroom poisoning. The early inhibition of protein synthesis for beta-amanitin might be useful for future experiments and research.en_US
dc.identifier.endpage732en_US
dc.identifier.issn1300-0144
dc.identifier.issn1303-6165
dc.identifier.issue5en_US
dc.identifier.startpage728en_US
dc.identifier.urihttps://app.trdizin.gov.tr/makale/TWpFek1UVTVPUT09
dc.identifier.urihttps://hdl.handle.net/20.500.12684/1316
dc.identifier.volume44en_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCerrahien_US
dc.titleEvaluation and comparison of alpha- and beta-amanitin toxicity on MCF-7 cell lineen_US
dc.typeArticleen_US

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