Sodyum azid (NaN3) ile muamele edilen arpa mutantlarının genetik çeşitliliğinin ISSR markörler ile belirlenmesi
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2020
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info:eu-repo/semantics/openAccess
Özet
Sodyum azid (NaN3) özellikle nokta mutasyon çalışmalarında yoğun olarak kullanılan kimyasal bir mutajendir. Buçalışmada, Finola altı sıralı arpa çeşidi, kimyasal bir mutajen olan sodyum azid ile muamele edilerek 14 adetmutant arpa hattı elde edilmiştir. Elde edilen hatlar arasındaki genetik farklılık, 5 adet basit tekrarlı diziler arasıpolimorfizm (Inter Simple Sequence Repeat, ISSR) markörü yardımıyla belirlenmiştir. Elde edilen sonuçlara göre5 ISSR marköründen 24 adet bant elde edilmiş ve bu bantlardan 18 adet polimorfik allel belirlenmiş ve ortalamapolimorfizm oranı %76.33 olarak saptanmıştır. Ortalama allel sayısı 4.8 olup, 6 bant ile UBC-808 en fazla bantüreten primer olurken, en az bant üreten primerler ise 4’er bant ile UBC-820 ve UBC-825 primerleri olmuştur.Kullanılan markörlere ait ortalama polimorfizm bilgi içeriği (PIC) 0.83 olarak hesaplanmış ve PIC değeri 0.78 ile0.86 aralığında değişmiştir. Markör verileri dendrogramda 2 ana grup oluşturmuştur. Birinci kümede Fnl-4, Fnl-8,Fnl-10, Fnl-11 ve Fnl-12 mutantları yer alırken, ikinci kümede ise Finola çeşidi ile birlikte diğer mutant hatlar yeralmaktadır. Temel bileşenler analizi, Finola çeşidi ve mutant hatlardan Fnl-5, Fnl-13 ve Fnl-14 hatlarının birbirinebenzemekle birlikte, bazı alleller bakımından farklı olduklarını ortaya koymuştur.
Sodium azide (NaN3) is widely used as a chemical mutagen in mutation studies, especially to induce point mutations. This study was carried out to determine genetic diversity of six-rowed barley cultivar Finola and 14 mutant lines induced by sodium azide mutagenesis agent and identified by using 5 Inter Simple Sequence Repeat (ISSR) markers. According to the results, 24 bands were produced by five ISSR markers, 18 of them were found polymorphic, and the average polymorphism rate was 76.33%. The average allele number was determined as 4.8, and UBC-808 marker had the highest allele number with 6 alleles, while UBC-820 and UBC-825 markers had the lowest allele numbers with 4 alleles. The average polymorphism information content of the markers used in the study was 0.83, and ranked from 0.78 to 0.86. A dendogram was created using marker data, and according to the dendogram, genotypes were divided into two main groups. Fnl-4, Fnl-8, Fnl-10, Fnl-11 and Fnl-12 mutants existed in the first group, while the others including Finola cultivar were in the second group. Based on the principal component analysis (PCA), Finola cultivar and mutant lines Fnl-5, Fnl-13 and Fnl-14 were evaluated as they are similar to Finola although, differed for some alleles
Sodium azide (NaN3) is widely used as a chemical mutagen in mutation studies, especially to induce point mutations. This study was carried out to determine genetic diversity of six-rowed barley cultivar Finola and 14 mutant lines induced by sodium azide mutagenesis agent and identified by using 5 Inter Simple Sequence Repeat (ISSR) markers. According to the results, 24 bands were produced by five ISSR markers, 18 of them were found polymorphic, and the average polymorphism rate was 76.33%. The average allele number was determined as 4.8, and UBC-808 marker had the highest allele number with 6 alleles, while UBC-820 and UBC-825 markers had the lowest allele numbers with 4 alleles. The average polymorphism information content of the markers used in the study was 0.83, and ranked from 0.78 to 0.86. A dendogram was created using marker data, and according to the dendogram, genotypes were divided into two main groups. Fnl-4, Fnl-8, Fnl-10, Fnl-11 and Fnl-12 mutants existed in the first group, while the others including Finola cultivar were in the second group. Based on the principal component analysis (PCA), Finola cultivar and mutant lines Fnl-5, Fnl-13 and Fnl-14 were evaluated as they are similar to Finola although, differed for some alleles
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