NF-kappa B and MAPKs are involved in resistin-caused ADAMTS-5 induction in human chondrocytes

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Küçük Resim

Tarih

2015

Dergi Başlığı

Dergi ISSN

Cilt Başlığı

Yayıncı

Canadian Soc Clinical Investigation

Erişim Hakkı

info:eu-repo/semantics/closedAccess

Özet

Purpose: Chronic inflammation is an important etiological factor in the development of arthritic diseases. Several factors contribute to aggregation of chronic inflammation, including the presence of excess adipose tissue. Methods: The putative induction mechanisms of ADAMTS-5 by resistin were investigated in normal primary human articular chondrocytes. Expression levels of the ADAMTS-5 gene were determined at several resistin doses and durations. Results: Human chondrocytes were activated and associated with upregulated ADAMTS-5 gene expression after exposure to resistin (also known as adipose tissue-specific secretary factor, ADSF). Release of ADAMTS-5 leads to joint cartilage degradation, a key event in the development of arthritic diseases rheumatoid arthritis (RA) and osteoarthritis (OA). Activation of chondrocytes was associated with upregulated NF-kappa B protein levels in a time-dependent fashion. Co-incubation of human chondrocytes with JNK and p38 inhibitors lead to abrogated levels of NF-kappa B, indicating that these MAPKs are important in the activation of chondrocytes after stimulation with resistin. Similarly, ADAMTS-5 expression levels were abrogated when co-incubated with p38, NF-kappa B, JNK, MEK and PI3K inhibitors. Our results demonstrate that resistin, released from adipose tissue, may be involved in the development of RA and OA in obese patients through degradation of joint cartilage via ADAMTS-5 released from activated chondrocytes.

Açıklama

Yaykasli, Emine/0000-0001-6471-0106; Yaykasli, Kursat/0000-0001-7550-6370; Bender, Onur/0000-0003-0691-3508
WOS: 000359119100021

Anahtar Kelimeler

Kaynak

Clinical And Investigative Medicine

WoS Q Değeri

N/A

Scopus Q Değeri

Cilt

38

Sayı

4

Künye