İskemik priapizm modelinde hiperbarik oksijen tedavisinin iskemi reperfüzyon hasarına etkileri: Deneysel çalışma
Yükleniyor...
Dosyalar
Tarih
2022
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Düzce Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
ÖZET Amaç: Biz bu çalışmada ratlarda deneysel bir iskemik priapism modeli oluşturarak hiperbarik oksijen tedavisinin (HBO) histopatolojik ve biyokimyasal parametreldeki etkisini gözlemlemeyi ve iskemik priapizm hastalığında bir tedavi alternatifi olarak kullanılıp kullanılamayacağını araştırmayı amaçladık. Gereç ve Yöntem: Bu çalışmada 4 grup olmak üzere toplam 34 adet Wistar-Albino cinsi erkek rat kullanıldı. Grup 1 (n:7) sham grubu olarak belirlendi.Grup 2 (I/R) 'de (n:10) priapizm modeli oluşturularak 4.saatin sonunda konstrüktör bant çıkarıldı ve reperfüzyon aşamasında ratlar 1 hafta boyunca bekletildi. Grup 3 (I/R+HBO) 'te (n:10) yine priapizm modeli oluşturulan ratlara reperfüzyon aşamasında denek hayvanları için özel olarak yapılmış oksijen tankında 7 gün boyunca günde 1 seans HBO tedavisi verildi. Grup 4 (HBO) 'te (n:7) ise model oluşturulmadan sağlıklı ratlara 7 gün HBO tedavisi verildi. Tüm gruplara işlemler sonunda penektomi yapıldı ve abdominal aortadan 3ml kan alındı. Penil doku örneklerinin yarısı histopatolojik olarak incelendi. Diğer yarısı homojenize edilerek biyokimyasal incelemeye alındı. Alınan homojenize doku ve kan örneklerinden katalaz (CAT) , glutatyon peroksidaz (GPX), endotelyal nitrik oksit sentetaz (eNOS) ve hipoksi ile indüklenebilir faktör 1a (HIF1A) çalışıldı. Gruplardan elde edilen veriler uygun istatiksel yöntemlerle karşılaştırıldı. Bulgular: Grup 2 'de polimorf nüveli lokositlerin (PMNL) aktivasyonu ve kronik inflamasyon açısından Grup 1 ve 4'e göre istatistiksel anlamlı yükseklik saptandı. Ayrıca Grup 3 'teki kronik inflamasyon seviyeleri Grup 2 'ye göre istatistiksel anlamlı daha düşük saptandı.(p<0,05) Grup 2 ile Grup 3 arasında hem sistemik kanda hem de penil dokuda eNOS, GPX, CAT ve HIF1A seviyeleri bakımından anlamlı fark saptanmadı. Tüm bulgulara bakıldığında HBO tedavisinin; priapizme bağlı oluşan kavernozal dokudaki iskemi reperfüzyon hasarında ne biyokimayasal ne de histopatolojik olarak hiçbir parametrede hasarı arttırmadığı saptandı. Sonuçlar: HBO tedavisi; kronik inflamasyonu azaltarak priapism hastalığının özellikle kronik sürecindeki etkilerini azaltmada alternatif ya da yardımcı bir tedavi seçeneği olabilir. Ancak bu konuda neredeyse hiç hayvan deneyinin olmamasından dolayı daha fazla denek sayısı ile yapılan daha geniş kapsamlı çalışmalara ihtiyaç vardır. Anahtar Kelimeler: Priapizm, iskemi reperfüzyon hasarı , hiperbarik oksijen
ABSTRACT Objective:We aimed to create an experimental ischemic priapism model in rats, to observe the effect of hyperbaric oxygen therapy (HBO) on histopathological and biochemical parameters and to investigate whether it can be used as a treatment alternative in ischemic priapism disease. Materials and Methods:. In this study, a total of 34 male Wistar-Albino rats were used in 4 groups. Group 1 (n:7) was determined as the sham group. In Group 2 (I/R) (n:10) , a priapism model was created, and the constrictor band was removed at the end of the 4th hour, and the rats were kept for 1 week during the reperfusion phase. In Group 3 (I/R+HBO) (n:10) , rats with a priapism model were given 1 session of HBO treatment per day for 7 days in an oxygen tank specially made for the experimental animals during the reperfusion phase. In Group 4(HBO) (n:7), HBO treatment was given to healthy rats for 7 days without creating a model. Penectomy was performed in all groups at the end of the procedures and 3 ml of blood was taken from the abdominal aorta. Half of the penile tissue samples were examined histopathologically. Catalase (CAT), glutathione peroxidase (GPX), endothelial nitric oxide synthetase (eNOS) and hypoxia-inducible factor 1a (HIF1A) were studied from homogenized tissue and blood samples. The data obtained from the groups were compared with appropriate statistical methods. Results: A statistically significant increase was found in Group 2 in terms of activation of polymorph nuclear leukocytes (PMNL) and chronic inflammation compared to Group 1 and Group 4. In addition, the chronic inflammation levels in Group 3 were found to be statistically significantly lower than those in Group 2. (p<0.05) There was no significant difference between Group 2 and Group 3 in terms of eNOS, GPX, CAT and HIF1A levels in both systemic blood and penile tissue. Considering all the findings, it was determined that HBO treatment did not increase the damage in any parameter, neither biochemically nor histopathologically, in ischemia-reperfusion injury in the cavernosal tissue due to priapism. Conclusions: HBO therapy may be an alternative or adjunctive treatment option to reduce the effects of priapism in the chronic process of priapism by reducing chronic inflammation. Keywords: Priapism,ischemic reperfusion injury,hyperbaric oxygen
ABSTRACT Objective:We aimed to create an experimental ischemic priapism model in rats, to observe the effect of hyperbaric oxygen therapy (HBO) on histopathological and biochemical parameters and to investigate whether it can be used as a treatment alternative in ischemic priapism disease. Materials and Methods:. In this study, a total of 34 male Wistar-Albino rats were used in 4 groups. Group 1 (n:7) was determined as the sham group. In Group 2 (I/R) (n:10) , a priapism model was created, and the constrictor band was removed at the end of the 4th hour, and the rats were kept for 1 week during the reperfusion phase. In Group 3 (I/R+HBO) (n:10) , rats with a priapism model were given 1 session of HBO treatment per day for 7 days in an oxygen tank specially made for the experimental animals during the reperfusion phase. In Group 4(HBO) (n:7), HBO treatment was given to healthy rats for 7 days without creating a model. Penectomy was performed in all groups at the end of the procedures and 3 ml of blood was taken from the abdominal aorta. Half of the penile tissue samples were examined histopathologically. Catalase (CAT), glutathione peroxidase (GPX), endothelial nitric oxide synthetase (eNOS) and hypoxia-inducible factor 1a (HIF1A) were studied from homogenized tissue and blood samples. The data obtained from the groups were compared with appropriate statistical methods. Results: A statistically significant increase was found in Group 2 in terms of activation of polymorph nuclear leukocytes (PMNL) and chronic inflammation compared to Group 1 and Group 4. In addition, the chronic inflammation levels in Group 3 were found to be statistically significantly lower than those in Group 2. (p<0.05) There was no significant difference between Group 2 and Group 3 in terms of eNOS, GPX, CAT and HIF1A levels in both systemic blood and penile tissue. Considering all the findings, it was determined that HBO treatment did not increase the damage in any parameter, neither biochemically nor histopathologically, in ischemia-reperfusion injury in the cavernosal tissue due to priapism. Conclusions: HBO therapy may be an alternative or adjunctive treatment option to reduce the effects of priapism in the chronic process of priapism by reducing chronic inflammation. Keywords: Priapism,ischemic reperfusion injury,hyperbaric oxygen
Açıklama
Anahtar Kelimeler
Priapizm, iskemi reperfüzyon hasarı, hiperbarik oksijen, Priapism,ischemic reperfusion injury,hyperbaric oxygen, Üroloji, Urology
