Investigation of interleukin-4 (IL-4) and leukotriene C4 synthase (LTC4S) genes in patients with asthma by next-generation sequencing analysis

Basit Öğe Kaydı
dc.contributor.authorEngin, Muhammet Mesut Nezir
dc.contributor.authorKilicaslan, Onder
dc.contributor.authorEroz, Recep
dc.contributor.authorOzdemir, Oner
dc.contributor.authorTemizkan, Ramazan Cahit
dc.contributor.authorKocabay, Kenan
dc.date.accessioned2025-10-11T20:47:38Z
dc.date.available2025-10-11T20:47:38Z
dc.date.issued2024
dc.departmentDüzce Üniversitesien_US
dc.description.abstractIntroduction: Asthma is the most common childhood chronic respiratory disease. There are many studies on asthma genetics today, but asthma genetics is still not clearly understood. As far as we know, there is no study in the literature evaluating the IL-4 or leukotriene C4 synthase (LTC4S) gene with the next generation sequencing method. Aim: In this study, we aimed to reveal the changes in IL-4 and LTC4S genes by next-generation sequencing analysis in patients followed up with the diagnosis of asthma. Material and methods: This is a prospective study conducted on 50 patients who were diagnosed with asthma and followed up by a specialist physician according to GINA criteria at an outpatient clinic of Pediatrics in a University Hospital. DNA isolation was performed, and IL-4 and LTC4S genes were scanned from the isolated DNA samples using the next generation sequencing method. In addition, demographic, clinical and laboratory data of the patients were recorded. Results: A total of 50 patients, 32 (64%) male and 18 (36%) female, were included in the study. Variation was detected in 31 (62%) of 50 patients included in our study. The variants c.-33C>T in intron 1, c.361-9C>A in intron 3, c.360+18 C>A in intron 3 and c.23G >A in exon 4 of the IL-4 gene were detected in the asthmatic case group. The variants c.59-10 C>A in intron 1, c.-33 C>T in intron 1, c.230-12 T>C in intron 3, c.361-9 C>A in intron 3 and c.312-16 T>C in intron 5 were identified in the LTC4S gene. Conclusions: In our study, we found 9 different genetic changes that may contribute to the pathogenesis of the disease in the presence of available data in patients with asthma. The genetic changes detected in IL-4 and LTC4S genes in our study will contribute to studies on the diagnosis, prognosis and treatment of asthma.en_US
dc.description.sponsorshipScientific Research Projects Unit of the Duezce University [2018.04.03.891]en_US
dc.description.sponsorshipFunding The research was funded by the Scientific Research Pro-jects Unit of the Duzce University, with the project num-bered 2018.04.03.891.en_US
dc.identifier.doi10.5114/pja.2024.145210
dc.identifier.endpage285en_US
dc.identifier.issn2353-3854
dc.identifier.issue4en_US
dc.identifier.startpage277en_US
dc.identifier.urihttps://doi.org/10.5114/pja.2024.145210
dc.identifier.urihttps://hdl.handle.net/20.500.12684/21489
dc.identifier.volume11en_US
dc.identifier.wosWOS:001410126000001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherTermedia Publishing House Ltden_US
dc.relation.ispartofAlergologia Polska-Polish Journal of Allergologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmzKA_WOS_20250911
dc.subjectasthmaen_US
dc.subjectchilden_US
dc.subjectIL-4 geneen_US
dc.subjectLTC4S geneen_US
dc.subjectnext generation sequencing analysisen_US
dc.titleInvestigation of interleukin-4 (IL-4) and leukotriene C4 synthase (LTC4S) genes in patients with asthma by next-generation sequencing analysisen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu