Effect of montelukast on hepatic and renal ischemia/reperfusion injury
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Tarih
2017
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Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Torakoabdominal aort anevrizması cerrahisinin sebep olabildiği splanknik iskemi, karaciğer ve/veya böbrek hasarının neden olduğu ciddi postoperatif komplikasyonlarla sonuçlanabilir. Bu çalışmada seçici tersinir bir CysLT1 reseptör antagonisti olan montelukastın, aortanın geçici oklüzyonuyla yaratılan böbrek ve karaciğer iskemi-reperfüzyon (I/R) hasarı üzerindeki koruyucu etkisini incelemek için tasarlandı.Gereç ve Yöntemler: Yirmi bir erkek Sprague-Dawley tipi sıçan, rasgele üç gruba (her grupta n7) ayrıldı; G1 (aort oklüzyonu ve montelukast uygulaması yok), G2 (45 dakika aort oklüzyonu; montelukast uygulması yok) ve G3 (45 dakika aort oklüzyonu, 10 mg/kg montelukast uygulaması). Reperfüzyonun 48. saatinde böbrek ve karaciğerden alınan doku örneklerinden histopatolojik değerlendirme ve HSP 70, interlökin-6 ve miyeloperoksidaz (MPO) ile immünohistokimyasal boyama yapıldı.Bulgular: Böbrekten alınan doku örnekleri tübüler hücre dejenerasyonu, sitoplazmik vakuol formasyonu, hemoraji ve tübüler dilatasyon açısındna incelendi ve gruplar arasında istatistiksel olarak anlamlı fark bulundu (P 0.001, P 0.017, P 0.001). G1 ve G3 gruplarında HSP-70 ile boyanmada G2 ye göre anlamlı fark saptandı (P 0.001). G3 karaciğer örneklerinde hepatosit dejenerasyonu G1 ve G2 den anlamlı olarak düşüktü (P 0.027).Sonuçlar: Yapılan çalışmanın bulguları, montelukastın böbrek ve karaciğer üzerinde koruyucu etkileri olduğunu histopatolojik ve immünohistokimyasal olarak göstermiştir. Montelukastın koruyucu etkileri, ilacın antioksidan ve antiinflamatuar etkilerine bağlanabilir
Aim: The thoracoabdominal aortic aneurysm surgery may cause splanchnic ischemia and may result in severe postoperative complications caused by liver and/or kidney injury. This study investigated the effect of temporary occlusion of the aorta on the development of I/R injury of liver and kidney and the protective effect of montelukast, a selective reverse CysLT1 receptor antagosit. Material and Methods: Twenty-one male Sprague-Dawley rats were randomly assigned to three groups (n7 per group) as G1 (no aortic occlusion and montelukast administration), G2 (45 min. aortic occlusion; no montelukast administration) and G3 (45 min. aortic occlusion, 10 mg/kg montelukast administration). At the 48th hour of reperfusion, kidney and liver samples were dissected for histopathological evaluation and immunohistochemical staining for HSP70, interleukin-6 and myeloperoxidase (MPO). Results: Tissue samples taken from the kidney showed significant intergroup differences in terms of tubular cell degeneration, cytoplasmic vacuole formation, hemorrhage and tubular dilatation (P 0.001, P 0.017, P 0.001, P 0.001 respectively). There was a significant difference with regards to HSP-70 stain (P 0.01) in favor of G1 and G3 with respect to G2. Hepatocyte degeneration in the liver samples of G3 was significantly lower than that of G1 and G2 (P 0.027).Conclusion: The findings of the current study demonstrated that montelukast has protective effects on both kidney and liver; and were confirmed histopathologically and immunohistochemically. The protective effects of montelukast can be attributed to its potential of anti-oxidative and anti-inflammatory actions
Aim: The thoracoabdominal aortic aneurysm surgery may cause splanchnic ischemia and may result in severe postoperative complications caused by liver and/or kidney injury. This study investigated the effect of temporary occlusion of the aorta on the development of I/R injury of liver and kidney and the protective effect of montelukast, a selective reverse CysLT1 receptor antagosit. Material and Methods: Twenty-one male Sprague-Dawley rats were randomly assigned to three groups (n7 per group) as G1 (no aortic occlusion and montelukast administration), G2 (45 min. aortic occlusion; no montelukast administration) and G3 (45 min. aortic occlusion, 10 mg/kg montelukast administration). At the 48th hour of reperfusion, kidney and liver samples were dissected for histopathological evaluation and immunohistochemical staining for HSP70, interleukin-6 and myeloperoxidase (MPO). Results: Tissue samples taken from the kidney showed significant intergroup differences in terms of tubular cell degeneration, cytoplasmic vacuole formation, hemorrhage and tubular dilatation (P 0.001, P 0.017, P 0.001, P 0.001 respectively). There was a significant difference with regards to HSP-70 stain (P 0.01) in favor of G1 and G3 with respect to G2. Hepatocyte degeneration in the liver samples of G3 was significantly lower than that of G1 and G2 (P 0.027).Conclusion: The findings of the current study demonstrated that montelukast has protective effects on both kidney and liver; and were confirmed histopathologically and immunohistochemically. The protective effects of montelukast can be attributed to its potential of anti-oxidative and anti-inflammatory actions
Açıklama
Anahtar Kelimeler
Tıbbi Araştırmalar Deneysel, Tıbbi Laboratuar Teknolojisi
Kaynak
Turkish Journal of Clinics and Laboratory
WoS Q Değeri
Scopus Q Değeri
Cilt
8
Sayı
2
