Investigation of Cytotoxic, Antimetastatic and Apoptotic Activities of Jerusalem Artichoke (Helianthus tuberosus L.) Extracts: Comparison with MCF-7 and MCF-12A Cells

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dc.contributor.authorBeyazyu, Fadime
dc.contributor.authorArslan, Emine
dc.contributor.authorKoygun, Gozde
dc.date.accessioned2024-08-23T16:07:21Z
dc.date.available2024-08-23T16:07:21Z
dc.date.issued2024en_US
dc.departmentDüzce Üniversitesien_US
dc.description.abstractJerusalem artichoke (Helianthus tuberosus L.) is an important medicinal plant due to its composition of several bioactive substances, high antioxidant activity, rich inulin content and produces functional food ingredients. Complementary alternative medicine therapies become more popular for cancer treatments because of higher efficiency, lower cost and minimum side effects. This study reported anti -cancer efficiency of both shell and tuber extract of Jerusalem artichoke (JA) on different breast cell lines with the purpose of discovering new alternative medicine therapies. Cytotoxic effects of JA were evaluated by XTT method by using different concentrations on different incubation times (24h, 48h and 72h). Invasion, adhesion and apoptotic studies were performed with IC50 values for 48h and 72h. JA extracts do not show cytotoxic activity on healthy human breast cell, while they induce tumoral cell death with dose and timedependent manner. The most effective doses of tuber and shell extracts were 108.8 mu M and 134.2 mu M at 48h and 20 mu M ve 5 mu M at 72h, respectively. It demonstrated robust anti -metastatic activity on MCF-7 cell lines because it inhibited adhesion, and invasion, significantly. TMRE and AnnexinV/7AAD staining were used for determine to mitochondrial membrane potential and nuclear morphology, respectively The results were found consistent with each other. Overall, this paper which first in literature demonstrates the anti -cancer efficacy of the JA both tuber and shell extract which can be proposed as a potent candidate for the treatment of breast cancer.en_US
dc.description.sponsorshipSelcuk University Scientific Research Project (BAP)en_US
dc.description.sponsorshipThis research was supported by Selcuk University Scientific Research Project (BAP) . This study was presented as an oral presentation 1st International Plant Biology Congress in 2018 (10-12May) and published as an abstract in the congrees book. F. B and E.A. conceived the original idea and build E.A. supervised the project. The experiments were performed by F.B and G.K. and I would like to thank Mert SUDAGIDAN for helping us with the devices for the experiments. This study was produced from the master thesis .en_US
dc.identifier.endpage303en_US
dc.identifier.issn2619-9149
dc.identifier.issue2en_US
dc.identifier.startpage293en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12684/14615
dc.identifier.volume27en_US
dc.identifier.wosWOS:001196996600019en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherKahramanmaras Sutcu Imam Univ Rektorluguen_US
dc.relation.ispartofKsu Tarim Ve Doga Dergisi-Ksu Journal of Agriculture and Natureen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAdhesionen_US
dc.subjectApoptosisen_US
dc.subjectCytotoxicityen_US
dc.subjectInvasionen_US
dc.subjectMCF-7 cellsen_US
dc.subjectBreast-Canceren_US
dc.subjectMigrationen_US
dc.subjectInvasionen_US
dc.subjectPlanten_US
dc.subjectConstituentsen_US
dc.subjectAdhesionen_US
dc.subjectBindingen_US
dc.titleInvestigation of Cytotoxic, Antimetastatic and Apoptotic Activities of Jerusalem Artichoke (Helianthus tuberosus L.) Extracts: Comparison with MCF-7 and MCF-12A Cellsen_US
dc.typeArticleen_US

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