Investigation of the effects of pinacidil and glibenclamide administration on HCN1, KCNT1, Kir 6.1, SUR1 gene expressions in hippocampus and cortex regions in epileptic rats

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dc.authorscopusid57967943200
dc.authorscopusid57219390008
dc.contributor.authorKılıç, Ümit
dc.contributor.authorSoytürk, H.
dc.date.accessioned2023-07-26T11:58:59Z
dc.date.available2023-07-26T11:58:59Z
dc.date.issued2022
dc.departmentDÜ, Sağlık Hizmetleri Meslek Yüksekokulu, Tıbbi Hizmetler ve Teknikler Bölümüen_US
dc.description.abstractThe purpose of this study was to look into the effects of pinacidil and glibenclamide on HCN1, KCNT1, Kir 6.1, and SUR1 gene expression in epileptic rats hippocampus and cortex. Male Wistar-Albino rats were used in this study. The drugs pinacidil and glibenclamide were utilized. Control, Epilepsy, Epilepsy-O, and Epilepsy-B were the five groups formed. The epileptic focus was created by intracortical administration of penicillin at a dose of 500 IU/2 ?l. Hippocampus and Cortex are removed from all animals and Kir 6.1, SUR1, HCN1, and KCNT1 gene expression levels were determined by qPCR. The SPSS 21 program was used for statistics. HCN1 gene expression level is equal in the hippocampus and cortex (p<0.05). KCNT1, SUR1, and KIR6.1 are more expressed in the hippocampus than in the cortex (p<0.05). In epilepsy groups, HCN1 gene expression level was found to be higher than KCNT1, SUR1, and KIR6.1 gene expression levels (p<0.05). KIR6.1, SUR1, gene expression levels decreased with the application of pinacidil and glibenclamide (p<0.05). SUR1 and KIR6.1 gene expression levels were considerably lower in the epilepsy pinacidil group compared to the other groups. The gene expression levels in the hippocampus were found to be considerably higher than in the cortex group, according to this study. The fact that HCN1 gene expression levels are significantly greater in both the brain and the hippocampus 24 hours following the commencement of epileptic convulsions suggests that preventive medication may be possible. © 2022 Ondokuz Mayis Universitesi. All rights reserved.en_US
dc.description.sponsorship2019.10.01.1408en_US
dc.description.sponsorshipThis study was financed by the Abant İzzet Baysal University Research Foundation (BAP-grant number: 2019.10.01.1408).en_US
dc.identifier.doi10.52142/omujecm.39.3.52
dc.identifier.endpage873en_US
dc.identifier.issn1309-4483
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-85142021264en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage868en_US
dc.identifier.urihttps://doi.org/10.52142/omujecm.39.3.52
dc.identifier.urihttps://hdl.handle.net/20.500.12684/13611
dc.identifier.volume39en_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorKılıç, Ümit
dc.language.isoenen_US
dc.publisherOndokuz Mayis Universitesien_US
dc.relation.ispartofJournal of Experimental and Clinical Medicine (Turkey)en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmz$2023V1Guncelleme$en_US
dc.subjectepilepsyen_US
dc.subjectgene expressionsen_US
dc.subjectHCN1en_US
dc.subjectKCNT1en_US
dc.subjectKir 6.1en_US
dc.subjectSUR1en_US
dc.subjectglibenclamideen_US
dc.subjecthyperpolarization activated cyclic nucleotide gated channelen_US
dc.subjecthyperpolarization activated cyclic nucleotide gated channel 1en_US
dc.subjectinwardly rectifying potassium channel subunit Kir6.1en_US
dc.subjectkcnt1 proteinen_US
dc.subjectpenicillin derivativeen_US
dc.subjectpinacidilen_US
dc.subjectsodium activated potassium channelen_US
dc.subjectsulfonylurea receptor 1en_US
dc.subjectunclassified drugen_US
dc.subjectanimal experimenten_US
dc.subjectanimal modelen_US
dc.subjectanimal tissueen_US
dc.subjectArticleen_US
dc.subjectbrain cortexen_US
dc.subjectcomparative studyen_US
dc.subjectcontrolled studyen_US
dc.subjectdrug mechanismen_US
dc.subjectepilepsyen_US
dc.subjectepileptic focusen_US
dc.subjectgene expression levelen_US
dc.subjecthippocampusen_US
dc.subjectmaleen_US
dc.subjectnonhumanen_US
dc.subjectraten_US
dc.subjectreal time polymerase chain reactionen_US
dc.titleInvestigation of the effects of pinacidil and glibenclamide administration on HCN1, KCNT1, Kir 6.1, SUR1 gene expressions in hippocampus and cortex regions in epileptic ratsen_US
dc.typeArticleen_US

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